Holocaust trauma reconstructed: Individual, familial, and social trauma.

Psychic trauma is characterized by ego damage, regression, and relative helplessness. Following a historic catastrophe, psychic trauma with ego damage is frequently encountered as cumulative trauma. Reconstruction was essential to the understanding of an adult patient initially unaware of his holocaust trauma. When the patient was a child, he and his parents were simultaneously traumatized. Intrapsychic intergenerational psychosocial and neurobiological factors were adverse codeterminants of his cumulative trauma. Isolated affects and fragmented memories required sequential connection in the context of family, identity, and society. Reconstruction importantly contributed to ego reintegration of a lost, lacerated childhood, and to adaptive mastery of trauma and conflict. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Evaluation of a Novel Thiol–Norbornene-Functionalized Gelatin Hydrogel for Bioprinting of Mesenchymal Stem Cells

Introduction: Three-dimensional bioprinting can be considered as an advancement of the classical tissue engineering concept. For bioprinting, cells have to be dispersed in hydrogels. Recently, a novel semi-synthetic thiolene hydrogel system based on norbornene-functionalized gelatin (GelNB) and thiolated gelatin (GelS) was described that resulted in the photoclick hydrogel GelNB/GelS. In this study, we evaluated the printability and biocompatibility of this hydrogel system towards adipose-tissue-derived mesenchymal stem cells (ASCs). Methods: GelNB/GelS was synthesized with three different crosslinking densities (low, medium and high), resulting in different mechanical properties with moduli of elasticity between 206 Pa and 1383 Pa. These hydrogels were tested for their biocompatibility towards ASCs in terms of their viability, proliferation and differentiation. The extrusion-based bioprinting of ASCs in GelNB/GelS-high was performed to manufacture three-dimensional cubic constructs. Results: All three hydrogels supported the viability, proliferation and chondrogenic differentiation of ASCs to a similar extent. The adipogenic differentiation of ASCs was better supported by the softer hydrogel (GelNB/GelS-low), whereas the osteogenic differentiation was more pronounced in the harder hydrogel (GelNB/GelS-high), indicating that the differentiation fate of ASCs can be influenced via the adaption of the mechanical properties of the GelNB/GelS system. After the ex vivo chondrogenic differentiation and subcutaneous implantation of the bioprinted construct into immunocompromised mice, the production of negatively charged sulfated proteoglycans could be observed with only minimal inflammatory signs in the implanted material. Conclusions: Our results indicate that the GelNB/GelS hydrogels are very well suited for the bioprinting of ASCs and may represent attractive hydrogels for subsequent in vivo tissue engineering applications.     

Cranial Base Synchondrosis Lacks PTHrP-Expressing Column-Forming Chondrocytes

The cranial base contains a special type of growth plate termed the synchondrosis, which functions as the growth center of the skull. The synchondrosis is composed of bidirectional opposite-facing layers of resting, proliferating, and hypertrophic chondrocytes, and lacks the secondary ossification center. In long bones, the resting zone of the epiphyseal growth plate houses a population of parathyroid hormone-related protein (PTHrP)-expressing chondrocytes that contribute to the formation of columnar chondrocytes. Whether PTHrP⁺ chondrocytes in the synchondrosis possess similar functions remains undefined. Using Pthrp-mCherry knock-in mice, we found that PTHrP⁺ chondrocytes predominantly occupied the lateral wedge-shaped area of the synchondrosis, unlike those in the femoral growth plate that reside in the resting zone within the epiphysis. In vivo cell-lineage analyses using a tamoxifen-inducible Pthrp-creER line revealed that PTHrP⁺ chondrocytes failed to establish columnar chondrocytes in the synchondrosis. Therefore, PTHrP⁺ chondrocytes in the synchondrosis do not possess column-forming capabilities, unlike those in the resting zone of the long bone growth plate. These findings support the importance of the secondary ossification center within the long bone epiphysis in establishing the stem cell niche for PTHrP⁺ chondrocytes, the absence of which may explain the lack of column-forming capabilities of PTHrP⁺ chondrocytes in the cranial base synchondrosis.     

野间宏笔下的战后日本人--小说《脸上的红月亮》及《崩溃感觉》主人公评析

该文通过分析日本战后派作家野间宏的两部小说即《脸上的红月亮》和《崩溃感觉》中的主人公形象,揭示了上个世纪三四十年代日本发动的侵略战争给日本国民,尤其是给一部分有知识、有一定良知的日本国民造成的不可痊愈的心灵创伤。作家从对人性摧残的层面强烈控诉了战争的罪恶。     

人股骨头软骨与半月板黏弹性分析

为了了解真实人体病态与正常关节软骨组织在力学性能上的差异,做了黏弹性分析实验．对于正常组和病态组的半月板,应力松弛曲线G(t)-1nt的斜率Kt分别是-0．1 17 9和-0．085 6,G(t)的终点值Ge分别是0．525 0和0．571 0．对于正常组和病态组的股骨头软骨,Kr分别是-0．101 4和～0．063 3,Ge分别是0．591 5和0．663 2．对于正常组和病态组的半月板,蠕变曲线J(t)-1nt的斜率Kc分别是0．208 O和0.109 5,J(t)的终点值Je分别是1．953 8和1．493 1．对于正常组和病态组的股骨头软骨,Kc分别是0．167 3和0．120 7,Je分别是1．854 9和1．599 8．病态组标本的应力松弛和蠕变量明显比正常组低,主要原因是胶原纤维变性和蛋白多糖减少．     

硫酸软骨素生产新工艺的研究

制备硫酸软骨素新工艺利用双酶酶解法提取。设计了正交试验，得到最优化反应条件。最优反应条件为温度50℃，pH8．6-8．9，加酶量0．03g／d1，收率达17．84％。     

Earthquakes and children: The role of psychologists with families and communities.

The 2008 Sichuan Province earthquake and 2005 Pakistan earthquake are examples of natural disasters that took an unimaginable toll on children. In such disaster management contexts, family members as well as health care and school personnel are the first-line responders and are natural sources of continued social support as children recover. Although psychologists have increasingly sophisticated understandings of post-disaster reactions and strategies for helping children and adolescents cope with trauma, models for responding to mass catastrophes are limited, particularly in geographically remote communities and in regions in which mental health services are stigmatizing. With children’s well-being subsequent to earthquakes inextricably linked to family and community, psychologists can make important contributions in 3 spheres: (a) coordinating and activating collaborations within children’s existing social contexts to develop post-earthquake interventions; (b) designing prevention and preparedness programs focused on the emotional needs of children in earthquake-prone communities; and (c) conducting research on interventions and recovery with particular attention to developmental stage, sociocultural-economic contexts, and the similarities versus differences across various types of disasters. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

切牙牙体、牙周膜的应力分析

本文应用有限单元法,从生物力学的角度,揭示切牙在外力作用下,牙体、牙周膜内部的应力分布,从而为分析牙齿、牙周膜损伤,引起临床病变,提供理论依据。     

Protective Effects of a Hyaluronan-Binding Peptide (P15-1) on Mesenchymal Stem Cells in an Inflammatory Environment

Mesenchymal stem cells (MSCs) obtained from various sources, including bone marrow, have been proposed as a therapeutic strategy for the improvement of tissue repair/regeneration, including the repair of cartilage defects or lesions. Often the highly inflammatory environment after injury or during diseases, however, greatly diminishes the therapeutic and reparative effectiveness of MSCs. Therefore, the identification of novel factors that can protect MSCs against an inflammatory environment may enhance the effectiveness of these cells in repairing tissues, such as articular cartilage. In this study, we investigated whether a peptide (P15-1) that binds to hyaluronan (HA), a major component of the extracellular matrix of cartilage, protects bone-marrow-derived MSCs (BMSCs) in an inflammatory environment. The results showed that P15-1 reduced the mRNA levels of catabolic and inflammatory markers in interleukin-1beta (IL-1β)-treated human BMSCs. In addition, P15-1 enhanced the attachment of BMSCs to HA-coated tissue culture dishes and stimulated the chondrogenic differentiation of the multipotential murine C3H/10T1/2 MSC line in a micromass culture. In conclusion, our findings suggest that P15-1 may increase the capacity of BMSCs to repair cartilage via the protection of these cells in an inflammatory environment and the stimulation of their attachment to an HA-containing matrix and chondrogenic differentiation.     

Psychophysical studies of repetitive wrist flexion and extension

Abstract

The purpose of this experiment was to investigate the feasibility of using psychophysical methods to determine maximum acceptable forces for various types and frequencies of repetitive wrist motion. Four adjustable work stations were built to simulate repetitive wrist flexion with a power grip, wrist flexion with a pinch grip, and wrist extension with a power grip. The study consisted of two separate experiments. Subjects worked for two days per week during the first experiment, and five days per week during the second experiment. Fifteen women completed the first experiment, working seven hours each day, two days per week, for 20 days. Repetition rates of 2, 5, 10, 15 and 20 motions per minute were used with each flexion and extension task. Maximum acceptable torques were determined for the various motions, grips, and repetition rates without dramatic changes in wrist strength, tactile sensitivity, or number of symptoms. Fourteen different women completed the second experiment, performing a wrist flexion motion (power grip) fifteen times per minute, seven hours per day, five days per week, for 23 days. There were no significant differences in maximum acceptable torque from day to day. However, the average maximum acceptable torque for a five days per week exposure was 36-3% lower than for the same task performed two days per week. Assuming that maximum acceptable torques decrease 36-3% for other repetition rates and motions, tables of maximum acceptable force were developed for female wrist flexion (power grip), female wrist flexion (pinch grip), and female wrist extension (power grip).