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41.
Marta Vallverdú-Prats Ramon Brugada Mireia Alcalde 《International journal of molecular sciences》2022,23(2)
Arrhythmogenic cardiomyopathy is a heritable heart disease associated with desmosomal mutations, especially premature termination codon (PTC) variants. It is known that PTC triggers the nonsense-mediated decay (NMD) mechanism. It is also accepted that PTC in the last exon escapes NMD; however, the mechanisms involving NMD escaping in 5′-PTC, such as reinitiation of translation, are less known. The main objective of the present study is to evaluate the likelihood that desmosomal genes carrying 5′-PTC will trigger reinitiation. HL1 cell lines were edited by CRISPR/Cas9 to generate isogenic clones carrying 5′-PTC for each of the five desmosomal genes. The genomic context of the ATG in-frame in the 5′ region of desmosomal genes was evaluated by in silico predictions. The expression levels of the edited genes were assessed by Western blot and real-time PCR. Our results indicate that the 5′-PTC in PKP2, DSG2 and DSC2 acts as a null allele with no expression, whereas in the DSP and JUP gene, N-truncated protein is expressed. In concordance with this, the genomic context of the 5′-region of DSP and JUP presents an ATG in-frame with an optimal context for the reinitiation of translation. Thus, 5′-PTC triggers NMD in the PKP2, DSG2* and DSC2 genes, whereas it may escape NMD through the reinitiation of the translation in DSP and JUP genes, with no major effects on ACM-related gene expression. 相似文献
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43.
Zhiqi Hao Sai Ma Lunping Liang Ting Feng Mengyuan Xiong Shangshu Lian Jingyan Zhu Yanjun Chen Lijun Meng Min Li 《International journal of molecular sciences》2022,23(7)
Drought and salinity stresses are significant abiotic factors that limit rice yield. Exploring the co-response mechanism to drought and salt stress will be conducive to future rice breeding. A total of 1748 drought and salt co-responsive genes were screened, most of which are enriched in plant hormone signal transduction, protein processing in the endoplasmic reticulum, and the MAPK signaling pathways. We performed gene-coding sequence haplotype (gcHap) network analysis on nine important genes out of the total amount, which showed significant differences between the Xian/indica and Geng/japonica population. These genes were combined with related pathways, resulting in an interesting mechanistic draft called the ‘gcHap-network pathway’. Meanwhile, we collected a lot of drought and salt breeding varieties, especially the introgression lines (ILs) with HHZ as the parent, which contained the above-mentioned nine genes. This might imply that these ILs have the potential to improve the tolerance to drought and salt. In this paper, we focus on the relationship of drought and salt co-response gene gcHaps and their related pathways using a novel angle. The haplotype network will be helpful to explore the desired haplotypes that can be implemented in haplotype-based breeding programs. 相似文献
44.
Davide Loizzo Savio Domenico Pandolfo Devin Rogers Clara Cerrato Nicola Antonio di Meo Riccardo Autorino Vincenzo Mirone Matteo Ferro Camillo Porta Alessandro Stella Cinzia Bizzoca Leonardo Vincenti Marco Spilotros Monica Rutigliano Michele Battaglia Pasquale Ditonno Giuseppe Lucarelli 《International journal of molecular sciences》2022,23(7)
Autophagy is a complex process involved in several cell activities, including tissue growth, differentiation, metabolic modulation, and cancer development. In prostate cancer, autophagy has a pivotal role in the regulation of apoptosis and disease progression. Several molecular pathways are involved, including PI3K/AKT/mTOR. However, depending on the cellular context, autophagy may play either a detrimental or a protective role in prostate cancer. For this purpose, current evidence has investigated how autophagy interacts within these complex interactions. In this article, we discuss novel findings about autophagic machinery in order to better understand the therapeutic response and the chemotherapy resistance of prostate cancer. Autophagic-modulation drugs have been employed in clinical trials to regulate autophagy, aiming to improve the response to chemotherapy or to anti-cancer treatments. Furthermore, the genetic signature of autophagy has been found to have a potential means to stratify prostate cancer aggressiveness. Unfortunately, stronger evidence is needed to better understand this field, and the application of these findings in clinical practice still remains poorly feasible. 相似文献
45.
以四川泡菜蔬菜原料——新鲜灯笼辣椒为对象,分析其表面附生乳酸菌Enterococcus mundtii(5 株)、Enterococcus faecalis(2 株)、Enterococcus hirae(5 株)、Lactococcus lactis(7 株)、Leuconostocmesenteroides(2 株)、Leuconostoc holzapfelii(3 株)和Weissella cibaria(79 株)对青霉素(penicillin,PEN)、红霉素(erythromycin,ERY)、四环素(tetracycline,TET)、链霉素(streptomycin,STR)和氯霉素(chloramphenicol,CHL)的抗生素耐药性和耐药基因分布,为制定合理的食品安全防控措施提供科学依据。研究表明:所有分离菌株均无PEN和ERY耐药性,其他种属部分菌株对TET、STR和CHL表现出单一、二重或三重耐药性。除E. hirae、E. faecalis和L. holzapfelii部分菌株对STR表现出单一耐药性外,所有L. mesenteroide菌株只表现出了STR单一耐药性;STR和TET、STR和CHL二重耐药菌株在E. faecalis、E. hirae、L. lactis和W. cibaria分离菌株中都有发现,但是STR、TET、CHL三重耐药菌株仅在W. cibaria中发现。聚合酶链式反应检测发现:除基因norA、sepA、tet(A)、tet(O)和aac(6’)-aph(2’)未被检出外,其他耐药菌株都有相应1 个或多个耐药基因被检出。多重耐药外排泵基因efrA、tolC、norC、sugE和mdfA较核糖体蛋白质保护和酶修饰基因检出率高,分别达到了49%、41%、48%、41%和47%。虽然辣椒表面附生乳酸菌的抗生素耐药基因在四川泡菜发酵过程中的扩散行为需要进一步研究,但根据食品加工过程安全规范标准,也应关注其表面附生的乳酸菌抗生素耐药性存在的潜在食品安全问题。 相似文献
46.
为调查猪源金黄色葡萄球菌(Staphylococcus aurues,S. aureus)对不同动物宿主具有凝血致病作用及是否携带S. aureus凝固酶基因vwb~C、vwb~Sbo4、vwb~Sov2和vwb~Seq1,本文以厦门地区生猪养殖场、屠宰场和终端市场分离的130株S. aureus为研究对象,通过血浆凝固酶实验研究菌株对兔血浆、牛血浆和羊血浆的凝血致病性,采用PCR方法对实验菌株进行vwb~C、vwb~(Sbo4)、vwb~(Sov2)和vwb~(Seq1)基因的扩增。结果表明,兔血浆、牛血浆和羊血浆的阳性凝血率分别为100.00%、28.46%、28.46%。S.aureus凝固酶vwb~C,vwb~(Sbo4)和vwb~(Sov2)基因的检出率分别为100.00%(130/130),26.92%(35/130)和26.93%(38/130),vwb~(Seq1)基因未检出;来自生猪养殖场S.aureus中vwb~(Sbo4)和vwb~(Sov2)基因的检出率以及对牛血浆和羊血浆的阳性凝血率明显高于另外两种来源S. aureus,有显著性差异(p0.05)。研究结果说明猪源S. aureus对不同动物宿主具有凝血致病作用,为猪源S. aureus对不同动物宿主的凝血致病作用和适应性提供研究基础。 相似文献
47.
Anay Priscilla David de Oliveira Mateus Matiuzzi da Costa Daniel Maia Nogueira Francesca Silva Dias 《International Journal of Dairy Technology》2020,73(2):345-356
Twenty isolates from milk and goat cheese were confirmed as Staphylococcus aureus. These isolates were characterised for phenotypic properties related to cell adhesion and for the presence of enterotoxin production, intercellular adhesion and β‐lactam resistance genes. Staphylococcus aureus L47 showed cell adhesion ability and positivity for the sec, sed, icaD, mecA and blaZ genes. Three antimicrobial compounds were tested singly or in pairs for growth control of strain L47: gallic acid (GA), nisin and essential oil (EO) of Croton heliotropiifolius (velame). At 24 h, EO and EO + nisin showed higher inhibitory activity against S. aureus L47 in goat milk. 相似文献
48.
49.
Zhen Zeng Tong Lyu Xin Jia Yue Chen Yingmin Lyu 《International journal of molecular sciences》2022,23(8)
During the growth cycle of lilies, assimilates undergo a process of accumulation, consumption and reaccumulation in bulbs and are transported and allocated between aboveground and underground organs and tissues. The sink–source relationship changes with the allocation of assimilates, affecting the vegetative growth and morphological establishment of lilies. In this study, the carbohydrate contents in different tissues of five critical stages during lily development were measured to observe the assimilates allocation. The results showed bulbs acted as the main source to provide energy before the budding stage (S3); after the flowering stage (S4), bulbs began to accumulate assimilates as a sink organ again. During the period when the plant height was 30cm with leaf-spread (S2), leaves mainly accumulated assimilates from bulbs through the symplastic pathway, while when leaves were fully expanded, it transformed to export carbohydrates. At the S4 stage, flowers became a new active sink with assimilates influx. To further understand the allocation of assimilates, 16 genes related to sugar transport and metabolism (ST genes) were identified and categorized into different subfamilies based on the phylogenetic analysis, and their protein physicochemical properties were also predicted. Tissue-specific analysis showed that most of the genes were highly expressed in stems and petals, and it was mainly the MST (monosaccharide transporter) genes that were obviously expressed in petals during the S4 stage, suggesting that they may be associated with the accumulation of carbohydrates in flowers and thus affect flower development process. LoSWEET14 (the Sugar will eventually be exported transporters) was significantly correlated with starch in scales and with soluble sugar in leaves. Sugar transporters LoHXT6 and LoSUT1 were significantly correlated with soluble sugar and sucrose in leaves, suggesting that these genes may play key roles in the accumulation and transportation of assimilates in lilies. In addition, we analyzed the expression patterns of ST genes under different abiotic stresses, and the results showed that all genes were significantly upregulated. This study lays a solid foundation for further research on molecular mechanism of sink–source change and response to abiotic stresses in lilies. 相似文献
50.