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81.
Nadire
zenver Onat Kadioglu Yujie Fu Thomas Efferth 《International journal of molecular sciences》2022,23(3)
Pigeon Pea (Cajanus cajan (L.) Millsp.) is a common food crop used in many parts of the world for nutritional purposes. One of its chemical constituents is cajanin stilbene acid (CSA), which exerts anticancer activity in vitro and in vivo. In an effort to identify molecular targets of CSA, we performed a kinome-wide approach based on the measurement of the enzymatic activities of 252 human kinases. The serine-threonine kinase WNK3 (also known as protein kinase lysine-deficient 3) was identified as the most promising target of CSA with the strongest enzymatic activity inhibition in vitro and the highest binding affinity in molecular docking in silico. The lowest binding affinity and the predicted binding constant pKi of CSA (−9.65 kcal/mol and 0.084 µM) were comparable or even better than those of the known WNK3 inhibitor PP-121 (−9.42 kcal/mol and 0.123 µM). The statistically significant association between WNK3 mRNA expression and cellular responsiveness to several clinically established anticancer drugs in a panel of 60 tumor cell lines and the prognostic value of WNK3 mRNA expression in sarcoma biopsies for the survival time of 230 patients can be taken as clues that CSA-based inhibition of WNK3 may improve treatment outcomes of cancer patients and that CSA may serve as a valuable supplement to the currently used combination therapy protocols in oncology. 相似文献
82.
83.
Hua Liu Wendan Chen Yushu Li Lei Sun Yuhong Chai Haixia Chen Haochen Nie Conglin Huang 《International journal of molecular sciences》2022,23(18)
The rapid growth of the global population has resulted in a considerable increase in the demand for food crops. However, traditional crop breeding methods will not be able to satisfy the worldwide demand for food in the future. New gene-editing technologies, the most widely used of which is CRISPR/Cas9, may enable the rapid improvement of crop traits. Specifically, CRISPR/Cas9 genome-editing technology involves the use of a guide RNA and a Cas9 protein that can cleave the genome at specific loci. Due to its simplicity and efficiency, the CRISPR/Cas9 system has rapidly become the most widely used tool for editing animal and plant genomes. It is ideal for modifying the traits of many plants, including food crops, and for creating new germplasm materials. In this review, the development of the CRISPR/Cas9 system, the underlying mechanism, and examples of its use for editing genes in important crops are discussed. Furthermore, certain limitations of the CRISPR/Cas9 system and potential solutions are described. This article will provide researchers with important information regarding the use of CRISPR/Cas9 gene-editing technology for crop improvement, plant breeding, and gene functional analyses. 相似文献
84.
Luis Daniel Sansores-Espaa Samanta Melgar-Rodríguez Rolando Vernal Bertha Arelly Carrillo-vila Víctor Manuel Martínez-Aguilar Jaime Díaz-Zúiga 《International journal of molecular sciences》2022,23(20)
Periodontitis is a chronic non-communicable disease caused by dysbiotic changes that affect the subgingival microbiota. During periodontitis, neutrophils play a central role in the initial recognition of bacteria, and their number increases with the appearance of the first signs of periodontal inflammation. Recent evidence has led to the proposition that neutrophils can also functionally polarize, determining selective activity patterns related to different diseases. Two well-defined neutrophil phenotypes have been described, the pro-inflammatory N1 subset and the suppressor N2 subset. To date, it has not been established whether these different neutrophil subtypes play a role in the pathogenesis of periodontitis. Thus, this scoping review aimed to determine whether there was evidence to suggest that the neutrophils present in periodontal tissues can be associated with certain phenotypes. The research question, population, concept, and context sought to identify original articles, in humans, that detected the presence of neutrophils in the periodontal tissues of people affected by periodontitis. Based on the search strategy, we found 3658 studies. After removing the papers with abstracts not related to the outcome measures and eligibility criteria, 16 articles were included for qualitative analysis. Several studies identified the presence of different neutrophil subsets, specifically, the naive, pro- and para-inflammatory, hyper-reactive and hyper-active, and high- and low-responder phenotypes. The existing evidence demonstrates the presence of pro-inflammatory, hyper-reactive and high-responder neutrophils in periodontal tissues affected with periodontitis. There is no evidence demonstrating the presence of the N1 or N2 phenotypes in periodontal tissues during periodontitis. However, the existence of pro-inflammatory phenotypes, which increase NETosis and degranulation, and increase the production of pro-inflammatory cytokines, could be suggestive of the N1 phenotypes. 相似文献
85.
Dexing Jiang Feng Wang Haizi Zhang Wenwen Gao Xi Tong Chuangen Lv Guoxiang Chen 《International journal of molecular sciences》2021,22(9)
Introduction of C4 photosynthetic traits into C3 crops is an important strategy for improving photosynthetic capacity and productivity. Here, we report the research results of a variant line of sorghum–rice (SR) plant with big panicle and high spikelet density by introducing sorghum genome DNA into rice by spike-stalk injection. The whole-genome resequencing showed that a few sorghum genes could be integrated into the rice genome. Gene expression was confirmed for two C4 photosynthetic enzymes containing pyruvate, orthophosphate dikinase and phosphoenolpyruvate carboxykinase. Exogenous sorghum DNA integration induced a series of key traits associated with the C4 pathway called “proto-Kranz” anatomy, including leaf thickness, bundle sheath number and size, and chloroplast size in bundle sheath cells. Significantly, transgenic plants exhibited enhanced photosynthetic capacity resulting from both photosynthetic CO2-concentrating effect and improved energy balance, which led to an increase in carbohydrate levels and productivity. Furthermore, such rice plant exhibited delayed leaf senescence. In summary, this study provides a proof for the feasibility of inducing the transition from C3 leaf anatomy to proto-Kranz by spike-stalk injection to achieve efficient photosynthesis and increase productivity. 相似文献
86.
C. A. Campbell Y. W. Jame O. O. Akinremi M. L. Cabrera 《Nutrient Cycling in Agroecosystems》1995,42(1-3):61-75
Quantification of N dynamics in the ecosystem has taken on major significance in today's society, for economic and environmental reasons. A major fraction of the available N in soils is derived from the mineralization of organic matter. For decades, scientists have attempted to quantify the rate at which soils mineralize N, but the complexity of the N cycle has made this a major task. Further, agronomists have long sought soil test methods that are practical, yet will provide accurate means of predicting the amounts and rates of release of N from soils. Such tests would allow us to make more precise fertilization decisions. This paper discusses the potentially mineralizable N concept, first promoted by Stanford and colleagues [61, 62, 64], and suggests how it may be incorporated into deterministic models, such as CERES and LEACHM, so as to provide more accurate estimates of N mineralization under field conditions. We also suggest how the potentially mineralizable N concept may be coupled to quick, routine laboratory methods of determining available soil N, such as the hot 2M KCl extracted NH4-N method recently developed by Gianello and Bremner [35], and used together with deterministic N models, such as CERES, for predicting probable fertilizer N requirements. 相似文献
87.
Md. Zahid Hossain 《Nutrient Cycling in Agroecosystems》2001,61(1-2):197-204
Bangladesh is an agricultural country. About 80% of the total population live in rural areas. The contribution of agriculture
to the gross domestic product is 30%. Rice is the major food crop while jute, sugarcane and tea are the main cash crops. Other
important crops are wheat, tobacco, pulses, vegetables and fruits. Overall productivity in Bangladesh is stagnating or declining.
The implication of yield stagnation or declining productivity is severe, since these trends have occurred despite rapid growth
in the use of chemical fertilisers. Depletion of soil organic matter is the main cause of low productivity, which is considered
one of the most serious threats to the sustainability of agriculture in Bangladesh. In Bangladesh, most soils have less than
17 g/kg and some soils have less than 10 g/kg organic matter. Farmers realise that there is a problem with soil fertility
related to organic matter depletion. Farmers say that organic matter increases yield, reduces the production cost, improves
crop growth and the economy, increases water-holding capacity and improves the soil structure. They recognise soil with higher
organic matter content by darker brownish to black in colour. Some farmers are using fast-growing trees such as Flemingia macrophyla, Ipilipil (Leucaen leucophala), Glyricidia sepium, Boga Medula (Tephrosia candida), Dhol Kolmi (Ipomoea fistulosa), etc., as living fences, which can be used as fuel, fertiliser and fodder. To increase the soil organic matter, farmers
use green manure crops, compost, quick compost, cow dung, azolla, etc. However, fuel for cooking purposes is limited and cow
dung and crop residues are largely used as fuel. Crop residues are also used as fodder for livestock. Farmers expressed the
wish to learn more about organic fertilizer management. However, sufficient food should be produced to keep pace with population
growth. To alleviate the hunger and poverty is to increase the intensity of agricultural production and maintain favorable
ecological conditions. Therefore, more organic matter should be used in the farmers' fields to sustain the soil fertility
in an intensive farming system.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
88.
Judged by their negative nutrient balances, low soil cover and low productivity, the predominant agro-pastoral farming systems
in the Sudano-Sahelian zone of West Africa are highly unsustainable for crop production intensification. With kaolinite as
the main clay type, the cation exchange capacity of the soils in this region, often less than 1 cmolc kg−1soil, depends heavily on the organic carbon (Corg) content. However, due to low carbon sequestration and to the microbe, termite
and temperature-induced rapid turnover rates of organic material in the present land-use systems, Corg contents of the topsoil
are very low, ranging between 1 and 8 g kg−1 in most soils. For sustainable food production, the availability of phosphorus (P) and nitrogen (N) has to be increased considerably
in combination with an improvement in soil physical properties. Therefore, the adoption of innovative management options that
help to stop or even reverse the decline in Corg typically observed after cultivating bush or rangeland is of utmost importance.
To maintain food production for a rapidly growing population, targeted applications of mineral fertilisers and the effective
recycling of organic amendments as crop residues and manure are essential. Any increase in soil cover has large effects in
reducing topsoil erosion by wind and water and favours the accumulation of wind-blown dust high in bases which in turn improves
P availability. In the future decision support systems, based on GIS, modelling and simulation should be used to combine (i)
available fertiliser response data from on-station and on-farm research, (ii) results on soil productivity restoration with
the application of mineral and organic amendments and (iii) our present understanding of the cause-effect relationships governing
the prevailing soil degradation processes. This will help to predict the effectiveness of regionally differentiated soil fertility
management approaches to maintain or even increase soil Corg levels.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
89.
90.
食源性疾病是全球重要的公共卫生问题,尤其以微生物危害因素中致病菌导致的危害最为严重,在基于风险的食品安全管理体系下,识别优先管控的危害因素极其重要的食物来源是防控食源性疾病的前提和关键。结合微生物分型和统计建模的归因方法是国际通用的以确定不同食物来源对食源性疾病相对贡献的技术方法,目前欧美等国已经建立了频率匹配和群体遗传学两大类基于微生物分型的归因方法模型,并将其结果转化为食品安全风险管控措施制定的科学依据,而我国目前正处于食源性疾病归因方法研究的初步探索阶段。本文系统梳理了目前国际广泛应用的归因方法的方法原理和模型,结合我国食源性疾病归因研究基础和现状,提出“建方法、合数据、搭平台、强交流”四个我国下阶段食源性疾病归因体系建设的工作方向,其中研发本土化微生物分型归因模型方法是解决我国确证管控核心和防控我国食源性疾病面临的挑战,最终实现食品安全和保障人民生命健康。 相似文献