结合水平集方法和PL主曲线分析的重叠细胞检测

提出了一种重叠细胞检测的新方法,该方法针对细胞图像处理中因细胞重叠而引起的过分割与欠分割现象这一难点,引入基于Mumford-Shah模型的水平集方法得到细胞的初始轮廓,采用EP（erosion-propagation）和PL主曲线相结合的图像分析方法得到目标的精确轮廓线。使用基于目标形状的图像配准方法和基于目标轮廓的分段特征分类器完成重叠细胞的检测。应用该方法对600张随机抽取的细胞图像中9374个细胞进行检测,实验正确率为91.83%,结果表明该方法能够有效地判断细胞是否重叠和重叠类型,为后续分割工作提供依据。     

悬液细胞图象识别的一种有效新算法

为进一步提高细胞识别的速度和识别率,本文研究一种细胞识别新算法。不同于其它的只对整个细胞形态进行一次图象处理的细胞识别方法,该算法在图象处理过程中,先后以细胞形态为重点和以细胞核形态为重点对原始图象进行两次图象处理,分别得到图象清晰度很高的整个细胞的二值图和细胞核的二值图。然后提取各项特征参数,对细胞图象进行识别。最后应用该算法对多幅细胞图象进行识别,结果表明该算法不但识别速度快,平均每幅图象的时间在3秒左右,而且识别率高达85%以上。     

P2P MMOG中计算任务的分配问题研究

P2P平台上的MMOG设计与C/S结构相比具有更好的可靠性和扩展性,但是需要新的数据存储和事件计算分配方法.本文提出小区的概念,并以小区为计算对象的基本分配单位,设计了节点可以自组织地寻找自己地计算和通信对象的方法.小区模型的方法通过确定节点的职能,对系统中的计算任务进行划分;而合理的计算分配保证了节点计算结果的正确性,以及进行安全的通信和数据发布;每个小区内部建立多个计算节点相互监督的多层计算模式,以确保系统的安全性.最后通过实验分析了该模型的计算和通信代价,指出在提高系统可靠性的同时,降低网络负载.     

基于可连Cell的无线传感器网络拓扑控制算法

在无线传感器网络的拓扑控制(TC)中,基于Cell的TC算法被认为是一类可以节省传感器节点能量并延长网络生命周期的方法,但是其需要较多的骨干网节点并且无法保证连通性.通过分析现今算法的内在局限性,提出了一种1-Con思想:当一个Cell的头节点被加入当前骨干网时,所有其可以连接的Cell使用该节点连入拓扑结构,然后此新骨干网递归地继续扩大.基于此思想,设计了一种基于可连Cell的拓扑控制算(CCTC),并从理论上证明:1)CCTC可以保证其所形成的拓扑结构维持网络连通;2)每一轮用于形成骨干网的工作节点非常少.CCTC的计算复杂度是线性的,空间复杂度和信息交换量都是常数量级.仿真实验同样显示,CCTC可以在提供良好鲁棒性和较少的消息交换的情况下,更有效地节省节点能耗并延长网络生命周期.     

嵌入式GPS／GIS系统设计的几个关键问题及解决方法

提出嵌入式GPS/GIS系统设计中的几个关键技术问题并给出解决方案。对GPS数据质量采用神经元辨识的方法进行控制;对大数据量底图数据采用一种新的地图综合方法建立多级显示机制,节省内存,提高图形显示效率;建立基于la-bel的空间算子实现方式,完成采集图斑的现场变更,减少内业数据处理的工作量。实际应用表明,这些方法能够满足嵌入式GPS/GIS系统应用于土地调查业务的需要,提高了调查效率。     

光寻址电位传感器及其应用

光寻址电位传感器(LAPS)是一种基于半导体光电效应的化学传感器,在稳定性、重复性、灵敏度、准确性和响应速度等方面具有优良的性能。介绍了LAPS的基本原理和测量方法,阐述了LAPS在细胞传感器、图像传感器、金属离子敏感薄膜传感器、气体传感器及生物医学领域中的应用,并对其存在问题和研究方向进行了总结和展望。     

一种新型三维敏感效应电容式SO2气体传感器设计

传统气体传感器只能以简单一维扩散方式对气体响应,扩散机理是建立在表面效应基础上的。提出了一种新型电容式SO2气体传感器。该传感器敏感结构由离散的微型圆柱体单元三维式扩散模型组成,模型上表面和侧面同时接触被测气体,扩大了与被测气体的接触面积。根据气敏薄膜的扩散响应理论,对传感器的响应时间作出分析,并与梳状电容式气体传感器特性相比较,说明新型传感器具有响应时间更短的优点。     

基于击键特征的手机用户身份认证系统

针对目前手机的高普及、高智能和安全性差的问题,统计并研究了不同手机用户使用手机的击键特征。依据数理统计知识发现其符合正态分布,进而设计和实现了基于击键特征的手机用户身份认证系统。经测试表明,该系统能显著提高手机安全性。     

Construction of human monoclonal single-chain Fv antibodies against small-cell lung cancer by phage display libraries derived from cell-immunized SCID mice engrafted with human peripheral blood lymphocytes

In this study, we describe a phage display strategy to obtain human monoclonal single-chain Fv (scFv) antibodies binding target cancer cell surface proteins. By developing a cancer cell immunization protocol for SCID mice engrafted with human peripheral blood lymphocytes in combination with an antibody phage display method, we have isolated phage antibodies binding small-cell lung cancer cell line H889 by subtractive selection. One of the isolated scFv antibodies, 12EAb, recognized the E2 component of pyruvate dehydrogenase complex (PDC-E2) by immunoprecipitation according to MALDI-TOF MS analysis. Furthermore, we have confirmed the plasma membrane localization of PDC-E2 in small-cell lung cancer cells by immunocytochemistry and cell surface protein biotinylation, although PDC-E2 is usually located in the mitochondrial matrix. These results, including unique localization of identified antigens, were obtained by proteomic approaches. The present methods can be applied to generate human monoclonal scFv antibodies against tumor cells and to identify new molecular targets for immunotherapy and markers for diagnosis.     

Isoform-specific expression and characterization of 14-3-3 proteins in human glioma tissues discovered by stable isotope labeling with amino acids in cell culture-based proteomic analysis

Human 14-3-3 proteins have isoform-specific expression and functions in different kinds of normal or tumor cells and tissues. However, the expression profiling of 14-3-3 proteins and isoform-specific biological functions are unclear in human glioma so far. In our study, the expression levels and characterization of 14-3-3 isoforms in human glioma tissues were investigated by a sensitive, accurate stable isotope labeling with amino acids in cell culture-based quantitative proteomic strategy. As a result, except unexpressed 14-3-3σ, the other six isoforms, with different expression levels, were existed in glioma tissues and para-cancerous brain tissues (PBTs). 14-3-3β and η were upregulated, whereas 14-3-3ζ was downregulated in glioma tissues compared with that in PBTs. And the other three isoforms 14-3-3ε, θ, and γ had similar expression levels in human glioma tissues and PBTs. Western blot and immunohistochemistry analysis were both consistent with the quantitative proteomic data. The loss of expression of 14-3-3σ was further discovered due to DNA high methylation in its coding region in glioma by methylation-specific PCR analysis. These results indicated that the four isoforms, including 14-3-3β, η, ζ, and σ, may play important roles in tumorigenesis of human glioma, which is probably used as potential biomarkers for diagnosis and targets for treatment of human gliomas in future.