基于RGD多肽掺杂聚吡咯-铟锡氧化物的仿生微电极构建及用于细胞生物学行为的电化学阻抗谱检测

构建一种基于RGD多肽分子掺杂聚吡咯膜修饰的铟锡氧化物微电极(PPy/RGD-ITO),并以此作为传感电极实现细胞生物学行为的电化学阻抗谱检测.采用光刻技术蚀刻感光干膜绝缘层制备ITO微电极;以含RGD模体的多肽分子作为吡咯电聚合唯一的掺杂阴离子,通过电化学共聚合方式在ITO微电极表面沉积PPy/RGD复合膜形成PPy/RGD-ITO微电极;原子力显微镜(AFM)、接触角测量仪和傅里叶变换红外光谱仪(FTIR)分别表征PPy/RGD复合膜的表面拓扑形貌、湿润性和组成成分;人肺癌细胞株A549铺展、粘附及增殖实验考察了PPy/RGD复合膜与细胞间的相互作用;以构建的PPy/RGD-ITO微电极作为传感电极,通过电化学阻抗谱技术对A549细胞粘附增殖行为及天然抗癌药物分子重楼皂苷I的细胞毒性进行了分析.结果显示,通过简单的电化学共聚合成功将RGD分子掺杂进PPy膜内,且PPy/RGD复合膜具有优异的表面物理性能;PPy基质膜内掺杂的RGD分子保留其生物活性,相比裸ITO电极和聚4-苯乙烯磺酸钠(PSS)掺杂的PPy膜,PPy/RGD复合膜能更好地促进A549细胞的铺展、粘附和增殖;由于PPy/RGD-ITO微电极表面A549细胞形态学变化可改变电极系统的阻抗谱特征,因此通过电化学阻抗谱技术可解析A549细胞粘附增殖行为学信息,同时可定量分析重楼皂苷I细胞毒性.因此,通过简单的电化学共聚合方法将生物活性RGD分子掺杂进PPy膜内制备出的PPy/RGD膜具有优良的生物相容性,可作为一种重要的仿生电极修饰材料用于构建电子系统和细胞生物学系统的耦合界面,未来可应用于细胞生物学行为及药物筛选研究.     

Coatings of indium tin oxide nanoparticles on various flexible polymer substrates: Influence of surface topography and oscillatory bending on electrical properties

Abstract— Coatings of indium tin oxide (ITO) nanoparticles on different flexible polymer substrates were investigated with respect to the achievable sheet resistance and their electrical behavior under oscillatory bending. As substrate materials, polyethyleneterephthalate (PET), polyethylenenaphthalate (PEN), polyetheretherketone (PEEK), and polyimide (PI) were chosen, the surface resistances on the different polymer substrates were compared as a function of annealing temperature and surface topography. The surface topography, which has a strong influence on the surface resistance, was characterized by means of a white‐light confocal (WL‐CF) microscope. On the PET substrate, which exhibits the smoothest surface, the coating of ITO nanoparticles shows the lowest sheet resistance of 2 kΩ/□ for a layer thickness of 3 μm and an annealing temperature of 200°C. Furthermore, the electrical behavior of coatings of ITO nanoparticles under oscillatory bending was investigated using a special device. These coatings show a cyclic change of the conductivity which can be explained by an alternating compression and extension of crack flanks under the applied stress. Due to the growing number of cracks with increasing number of cycles, a decrease of the conductivity is observed in the bent state as well as in the balanced state. For a small bending radii, the decrease of the conductivity is stronger due to more cracks caused by the higher tensile stresses in the layer. The electrical behavior of the coatings of the annealed ITO nanoparticles on PET films under oscillatory bending was compared with commercially available sputtered ITO coatings. The annealed coatings of ITO nanoparticles demonstrate better electrical properties under oscillatory bending than coatings of sputtered ITO. The different electrical behavior under oscillatory bending can be related to differences in crack formation.     

冰花镀锡工艺探讨

阐述冰花镀锡的工艺流程、要点，以及不良镀层的退除。     

气相色谱法分析E型电热蚊香片中炔丙菊酯的含量

采用填充柱气相色谱法,选用以OV-101为固定相的玻璃柱,用内标法测定E型电热蚊香片中炔丙菊酯的含量。方法的线性相关系数为0.9999,标准偏差为0.043,变异系数为0.34%,回收范围为99.87%～100.07%。     

酞酸二丁酯表面印迹聚合物的合成及其吸附性能

以酞酸二丁酯(DBP)作为模板分子,甲基丙烯酸作为功能单体,乙二醇二甲基丙烯酸酯(EDMA)作为交联剂,在硅胶表面合成分子印迹聚合物(MIPs)。通过红外、热重分析、比表面积测定等对聚合物进行分析和表征;采用静态吸附法对水相中的印迹聚合物的吸附性能进行考察.红外结果表明硅胶表面存在对模板分子具有识别功能的官能团;热重分析证明其良好的热稳定性;通过测定计算印迹膜厚度;静态吸附实验说明印迹聚合物具有良好的吸附性能.     

基于Tin改进的LiDAR滤波算法

现有典型的基于Tin的滤波算法只对本格网内的数据进行处理,没有考虑邻近地形的影响,使得滤波处理不能取得很好的效果。本文以待处理格网为中心,将待处理格网最低点与领域格网最低点构成三角网,再对格网内的数据与所在三角形的三个顶点进行比较,判断是否符合阈值来确定是否为地面点。实验的结果表明对改进后的三角网迭代的算法大大改善了滤波效果,提高了滤波处理结果的准确性。     

四(对氯苄基)锡(p-Cl-PhCH2)4Sn的合成及晶体结构

利用对氯苄基氯和锡反应,合成了四(对氯苄基)锡,用X-射线单晶衍射测定了该化合物的晶体结构.化合物晶体为正交晶系,空间群 fdd2,a=2.1237(9)nm,b=2.2127(11)nm,c=1.1235(5),α=90°,β=90°,γ=90°,Z=8,V=5.279(4)nm3,Dc=1.562g/cm3,μ=1.388 mm-1,R=0.0396,wR=0.0690.锡原子呈畸变四面体构型.分子间通过Cl…Cl近距作用,形成一维链状结构.     

甲基橙指示还原终点铈量法测定铁矿石中全铁含量

铁矿石经HCl-KF HCO4溶样后，用二氯化锡还原Fe^3 为Fe^2 ，以甲基橙做指示剂指示还原终点，肺铈量法测定铁矿石中全铁的含量。方法简便，快速，终点每锐，精密度好，准确度高。     

A Novel Instrument for the Measurement of the Thermal Conductivity of Molten Metals. Part II: Measurements

New measurements of the thermal conductivity of molten mercury, gallium, tin, and indium are reported up to 750 K. The measurements are performed in a novel transient hot-wire instrument described elsewhere. The present experimental technique overcomes problems of convection, and it is shown that it operates in an absolute way in accord with a theoretical model. The uncertainty of the thermal conductivity results is estimated to be ± 2%, which is superior to that achieved in most earlier work. The low uncertainty of the present experimental results has allowed us to test the only significant theory for the thermal conductivity of molten metals, which relates this property to the electrical conductivity. The pattern of results among the four metals indicates that further theoretical developments would be warranted.     

Preparation and CO gas-sensing behavior of Au-doped SnO2 sensors

A study on the low-temperature CO gas sensors based on Au/SnO₂ thick film was reported. Au/SnO₂ powders, with different Au loading from 0.36 to 3.57 wt%, were prepared by a deposition-precipitation method. Thick films were fabricated from Au/SnO₂ powders. The Au/SnO₂ thick-film sensors exhibited high sensitivity to CO gas at relatively low operating temperature (83-210 °C). We also reported the effect of the Au loading in Au/SnO₂ on the CO gas sensing behavior. The optimal Au loading in as-prepared Au/SnO₂ was 2.86 wt%.