Embedding in Spurr's resin is a good choice for immunolabelling after freeze drying as shown with chemically unfixed dendritic cells

Immunocytochemical reactions on biological specimens depend on many factors, the most crucial one being the maintenance of antigenicity. Antigens are vulnerable at each stage during preparation for electron microscopy. One of the least traumatic methods of preparing biological tissues for post‐embedding immunolabelling includes the following steps: (1) physical stabilization of the native biological material by rapid freezing (cryofixation) and keeping the immobilized biological sample at low temperature, thereby avoiding any movements of water, ions and macromolecules; (2) dehydrating the frozen biological material by freeze‐drying at low temperature; (3) embedding of the dehydrated specimen. Here we show that embedding of chemically unfixed dendritic cells in Spurr's resin after cryofixation and freeze‐drying enables the conservation of fine ultrastructure without cell distortion or shrinkage. Furthermore, we demonstrate the feasibility of protein localization in ultrathin sections by immunolabelling of the major histocompatibility class II molecules.     

基于深度强化学习的维吾尔语人称代词指代消解

针对深度神经网络模型仅学习当前指代链语义信息忽略了单个指代链识别结果的长期影响问题,提出一种结合深度强化学习（deep reinforcement learning）的维吾尔语人称代词指代消解方法.该方法将指代消解任务定义为强化学习环境下顺序决策过程,有效利用之前状态中先行语信息判定当前指代链指代关系.同时,采用基于整体奖励信号优化策略,相比于使用损失函数启发式优化特定的单个决策,该方法直接优化整体评估指标更加高效.最后在维吾尔语数据集进行实验,实验结果显示,该方法在维吾尔语人称代词指代消解任务中的F值为85.80%.实验结果表明,深度强化学习模型能显著提升维吾尔语人称代词指代消解性能.     

一种新的多嵌入延迟相空间重构方法

对文献[5]提出的Cao方法进行了改进,提出一种新的多嵌入延迟相空间重构方法,不仅能够判断最小嵌入维数,还能够确定最佳时间延迟矢量。Cao方法的应用领域得以扩展,使得相空间重构问题可以在一个判断标准下完成。数值仿真结果表明,该改进方法在处理包含多个强烈周期成分的信号时比目前常用的单一嵌入延迟方法有明显优势。     

核方法与流形学习在心电识别中的研究

结合核方法和局部线性嵌入（LLE）方法,提出了一种基于核局部线性嵌入方法,该方法克服了局部线性嵌入方法由于心电特征分布不均衡造成的不稳定问题。结合支持向量机在MIT-BIH心律失常标准数据库进行实验,并利用PCA和LLE进行特征提取比较,验证了该方法的有效性及优势。     

On Embedding of a Hypercube in a Completely Overlapping Network

In this paper a new theoretical network called Completely Overlapping Network or CON is introduced. We show that a d-dimensional hypercube network can be embedded into this new network. Taking advantage of faster and more powerful processors and a large gap between communication and computation speeds, we also show that the embedded d-dimensional hypercube can be scaled using latency hiding. Related mathematical properties are also shown for optimal scaling.     

结合二次差值扩展与插值扩展的可逆图像水印算法

为了提高现有可逆图像水印算法的视觉质量和嵌入 率,提出了一种结合二次差值扩展 和插值扩展的可逆图像水印算法。首先去除原始图像中的0和255像素 点;然后对去除0和255 像素点后构成的新图像进行互不重叠块划分并对划分出的每个图像块进行插值扩展;接着将 前一半置乱后的水印信息利用一次差值扩展嵌入到经插值扩展生成的图像块中;最后利用二 次差值扩展将剩余的一半水印信息嵌入到之前生成的水印图像中,去除扩展的像素点和并上 之前已去除的0和255像素点,以此生成最终的水印图像。实验结果表 明该算法不仅嵌入率高 ,而且有较高的视觉质量(平均PSNR值高达77.91 dB),能实现原始图像的完全恢复。和 其它基于差值扩展的可逆图像水印算法相比具有一定的优势,无需考虑嵌入区域的平滑度。     

虚拟网络映射高效节能运输模型及算法

网络虚拟化使得智能能量感知网络部署成为可能,已有研究忽略了节点映射能耗最优化。本文把节点映射能耗优化问题转化为生产地与销售地之间物资运输代价最优化问题,建立高效节能节点映射运输模型。根据最大元素法,提出了混合一阶段与两阶段映射算法,在链路映射的约束下找到节点分配最小能耗代价最优解;利用主动休眠策略,提出了基于运输模型的主动休眠虚拟网络映射节能算法;利用节点可重复映射技术,提出了基于运输模型的节点可重复映射算法,进一步提高了底层网络资源休眠数量。仿真结果验证了本文所提算法能够显著降低系统能耗,适合大规模高效节能虚拟网络映射。     

微生物固定法降解含油污水的研究进展

随着微生物固定化技术的发展,越来越多的研究成果应用于环境治理领域。固定化技术在废水生物治理领域中具有独特的优势和巨大的潜力。本文主要介绍了吸附法和包埋法这两种固定化方法载体的选择和制备方法,探讨了固定化技术对微生物生理特性的影响,综述了固定化技术在修复石油污染方面的应用,总结了固定化微生物技术在实际应用中存在的不足。     

Neighbourhood preserving discriminant embedding in face recognition

In this paper, we present an effective technique on discriminative feature extraction for face recognition. The proposed technique incorporates Graph Embedding and the Fisher’s criterion where we call it as Neighbourhood Preserving Discriminant Embedding (NPDE). Utilizing the Graph Embedding criterion, the underlying nonlinear face data structure is revealed as representative and discriminative features for analysis. We employ Neighbourhood Preserving Embedding (NPE) for the purpose. NPE takes into account the restriction that neighbouring points in the high-dimensional space must remain within the same neighbourhood in the low dimension space and be located in a similar relative spatial situation (without changing the local structure of the nearest neighbours of each data point). Furthermore, by taking the advantage of the Fisher’s criterion, the discriminating power of NPDE is further boosted. Based on this intuition, NPDE obtains better discriminative capability and experimentally verified in ORL, PIE and FRGC.     

面向政治领域的事理图谱构建

事理图谱是一种描述事件之间顺承、因果等关系的事理演化逻辑有向图,它蕴含了丰富的事件间关系,在各领域都具有重要的研究意义和应用价值.当前研究主要集中于公开域的事件抽取上,而在特定领域,如政治领域,因其事件类型和事件内容较为复杂,相关研究十分有限.该文旨在构建面向政治领域的事理图谱,针对政治事件抽取中存在的语料匮乏、标准缺...