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排序方式: 共有911条查询结果,搜索用时 343 毫秒
41.
Alexander Schriewer Kelly D. Goodwin Christopher D. Sinigalliano Annie M. Cox David Wanless Jakob Bartkowiak Darcy L. Ebentier Kaitlyn T. Hanley Jared Ervin Louise A. Deering Orin C. Shanks Lindsay A. Peed Wim G. Meijer John F. Griffith Jorge SantoDomingo Jennifer A. Jay Patricia A. Holden Stefan Wuertz 《Water research》2013
The contribution of fecal pollution from dogs in urbanized areas can be significant and is an often underestimated problem. Microbial source tracking methods (MST) utilizing quantitative PCR of dog-associated gene sequences encoding 16S rRNA of Bacteroidales are a useful tool to estimate these contributions. However, data about the performance of available assays are scarce. The results of a multi-laboratory study testing two assays for the determination of dog-associated Bacteroidales (DogBact and BacCan-UCD) on 64 single and mixed fecal source samples created from pooled fecal samples collected in California are presented here. Standardization of qPCR data treatment lowered inter-laboratory variability of sensitivity and specificity results. Both assays exhibited 100% sensitivity. Normalization methods are presented that eliminated random and confirmed non-target responses. The combination of standardized qPCR data treatment, use of normalization via a non-target specific Bacteroidales assay (GenBac3), and application of threshold criteria improved the calculated specificity significantly for both assays. Such measures would reasonably improve MST data interpretation not only for canine-associated assays, but for all qPCR assays used in identifying and monitoring fecal pollution in the environment. 相似文献
42.
为了研究准噶尔盆地南缘博格达山前带二叠系芦草沟组的油气来源,分析博格达山前带油气勘探潜力,系统采集了准噶尔盆地南缘博格达山前带新吉参1井和博参1井的烃源岩、天然气和原油样品和邻区主要油气田各层系的烃源岩和原油样品,并利用碳同位素和生物标志化合物技术对研究区油气地球化学特征和来源进行了分析,研究结果清晰显示研究区的原油与该区二叠系芦草沟组烃源岩的饱和烃组成相似,具有较好的亲缘性。同时,其生物标志化合物指标与邻区侏罗系、二叠系、石炭系烃源岩和原油差异明显,综合认为研究区发育自生自储型油气藏,油源来自于二叠系芦草沟组,具有较大勘探潜力。 相似文献
43.
塔里木盆地中上奥陶统油源层地球化学研究 总被引:47,自引:1,他引:47
通过对塔里木盆地古生界地层的沉积岩石学、有机地球化学、有机岩石学以及测井地质学等的综合研究,发现在满加尔凹陷南北斜坡带及柯坪露头区发育的中上奥陶统泥灰岩、泥页岩具有有机质丰度高(TOC 0.5%~5.54%)、有机成熟度适中(VRE 0.8%~1.3%)的特点,且具有一定的厚度分布。这套源岩的发育主要受上升洋流引起的高有机生产力带的控制,其生烃母质的构成具有浮游和底栖植物双重性。根据三芳甲藻甾烷和24-降胆甾烷等具有特殊生源和时代意义的生物标志物的油-岩对比分析和生烃史分析结果,证实目前在台盆区发现的工业性油流主要来源于这套地层。 相似文献
44.
Christina Jane Vellan Jaime Jacqueline Jayapalan Boon-Koon Yoong Azlina Abdul-Aziz Sarni Mat-Junit Perumal Subramanian 《International journal of molecular sciences》2022,23(4)
Pancreatic ductal adenocarcinoma (PDAC), a highly aggressive malignancy with a poor prognosis is usually detected at the advanced stage of the disease. The only US Food and Drug Administration-approved biomarker that is available for PDAC, CA 19-9, is most useful in monitoring treatment response among PDAC patients rather than for early detection. Moreover, when CA 19-9 is solely used for diagnostic purposes, it has only a recorded sensitivity of 79% and specificity of 82% in symptomatic individuals. Therefore, there is an urgent need to identify reliable biomarkers for diagnosis (specifically for the early diagnosis), ascertain prognosis as well as to monitor treatment response and tumour recurrence of PDAC. In recent years, proteomic technologies are growing exponentially at an accelerated rate for a wide range of applications in cancer research. In this review, we discussed the current status of biomarker research for PDAC using various proteomic technologies. This review will explore the potential perspective for understanding and identifying the unique alterations in protein expressions that could prove beneficial in discovering new robust biomarkers to detect PDAC at an early stage, ascertain prognosis of patients with the disease in addition to monitoring treatment response and tumour recurrence of patients. 相似文献
45.
Garrett Jones Tae Jin Lee Joshua Glass Grace Rountree Lane Ulrich Amy Estes Mary Sezer Wenbo Zhi Shruti Sharma Ashok Sharma 《International journal of molecular sciences》2022,23(4)
The tear film is a multi-layer fluid that covers the corneal and conjunctival epithelia of the eye and provides lubrication, nutrients, and protection from the outside environment. Tear fluid contains a high concentration of proteins and has thus been recognized as a potential source of biomarkers for ocular disorders due to its proximity to disease sites on the ocular surface and the non-invasive nature of its collection. This is particularly true in the case of dry eye disease, which directly impacts the tear film and its components. Proteomic analysis of tear fluid is challenging mainly due to the wide dynamic range of proteins and the small sample volumes. However, recent advancements in mass spectrometry have revolutionized the field of proteomics enabling unprecedented depth, speed, and accuracy, even with small sample volumes. In this study using the Orbitrap Fusion Tribrid mass spectrometer, we compared four different mass spectrometry workflows for the proteomic analysis of tear fluid collected via Schirmer strips. We were able to establish a method of in-strip protein digestion that identified >3000 proteins in human tear samples from 11 healthy subjects. Our method offers a significant improvement in the number of proteins identified compared to previously reported methods without pooling samples. 相似文献
46.
Samir Abu-Rumeileh Peggy Barschke Patrick Oeckl Simone Baiardi Angela Mammana Andrea Mastrangelo Mhd Rami Al Shweiki Petra Steinacker Anna Ladogana Sabina Capellari Markus Otto Piero Parchi 《International journal of molecular sciences》2022,23(4)
Proenkephalin (PENK) and prodynorphin (PDYN) are endogenous opioid peptides mainly produced in the striatum and, to a lesser extent, in the cerebral cortex. Dysregulated metabolism and altered cerebrospinal fluid (CSF) levels of PENK and PDYN have been described in several neurodegenerative diseases. However, no study to date investigated these peptides in the CSF of sporadic Creutzfeldt–Jakob disease (sCJD). Using liquid chromatography-multiple reaction monitoring mass spectrometry, we evaluated the CSF PDYN- and PENK-derived peptide levels in 25 controls and 63 patients with sCJD belonging to the most prevalent molecular subtypes (MM(V)1, VV2 and MV2K). One of the PENK-derived peptides was significantly decreased in each sCJD subtype compared to the controls without a difference among subtypes. Conversely, PDYN-derived peptides were selectively decreased in the CSF of sCJD MV2K, a subtype with a more widespread overall pathology compared to the sCJD MM(V)1 and the VV2 subtypes, which we confirmed by semiquantitative analysis of cortical and striatal neuronal loss and astrocytosis. In sCJD CSF PENK and PDYN were associated with CSF biomarkers of neurodegeneration but not with clinical variables and showed a poor diagnostic performance. CSF PDYN and PENK-derived peptides had no significant diagnostic and prognostic values in sCJD; however, the distinct marker levels between molecular subtypes might help to better understand the basis of phenotypic heterogeneity determined by divergent neuronal targeting. 相似文献
47.
48.
49.
Pablo J. Giraudi Noel Salvoza Deborah Bonazza Carlo Saitta Daniele Lombardo Biagio Casagranda Nicol de Manzini Teresa Pollicino Giovanni Raimondo Claudio Tiribelli Silvia Palmisano Natalia Rosso 《International journal of molecular sciences》2022,23(5)
Fibrosis is the strongest predictor for disease-specific mortality in non-alcoholic fatty liver diseases (NAFLD), but the need for liver biopsy limits its diagnosis. We assessed the performance of plasma ficolin-2 (FCN-2) as a biomarker of fibrosis identified by an in silico discovery strategy. Two hundred and thirty-five morbidly obese (MO) subjects with biopsy-proven NAFLD stratified by fibrosis stage (F0, n = 44; F1, n = 134; F2, n = 46; F3/F4, n = 11) and 40 cirrhotic patients were enrolled. The cohort was subdivided into discovery (n = 76) and validation groups (n = 159). The plasma level of FCN-2 and other candidate markers was determined. FCN-2 was inversely correlated with the stage of liver fibrosis (ρ = −0.49, p < 0.001) independently of steatosis (p = 0.90), inflammation (p = 0.57), and ballooning (p = 0.59). In the global cohort, FCN-2 level decreased significantly in a stepwise fashion from F0/F1 (median 4753 ng/mL) to F2–F3–F4 (2760 ng/mL) and in cirrhotic subjects (1418 ng/mL). The diagnostic performance of FCN-2 in detecting F ≥ 2 was higher than other indexes (APRI, FIB-4) (AUROC 0.82, 0.68, and 0.6, respectively). The accuracy improved when combined with APRI score and HDL values (FCNscore, AUROC 0.85). Overall, the FCN-2 plasma level can accurately discriminate liver fibrosis status (minimal vs. moderate/advanced) significantly improving the fibrosis diagnostic algorithms. 相似文献
50.
Irina Lousa Flvio Reis Alice Santos-Silva Luís Belo 《International journal of molecular sciences》2022,23(6)
Chronic kidney disease (CKD) has been recognized as a global public health problem. Despite the current advances in medicine, CKD-associated morbidity and mortality remain unacceptably high. Several studies have highlighted the contribution of inflammation and inflammatory mediators to the development and/or progression of CKD, such as tumor necrosis factor (TNF)-related biomarkers. The inflammation pathway driven by TNF-α, through TNF receptors 1 (TNFR1) and 2 (TNFR2), involves important mediators in the pathogenesis of CKD. Circulating levels of TNFRs were associated with changes in other biomarkers of kidney function and injury, and were described as predictors of disease progression, cardiovascular morbidity, and mortality in several cohorts of patients. Experimental studies describe the possible downstream signaling pathways induced upon TNFR activation and the resulting biological responses. This review will focus on the available data on TNFR1 and TNFR2, and illustrates their contributions to the pathophysiology of kidney diseases, their cellular and molecular roles, as well as their potential as CKD biomarkers. The emerging evidence shows that TNF receptors could act as biomarkers of renal damage and as mediators of the disease. Furthermore, it has been suggested that these biomarkers could significantly improve the discrimination of clinical CKD prognostic models. 相似文献