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71.
72.
E Aida Pea‐Ramos Youling L Xiong Guillermo E Arteaga 《Journal of the science of food and agriculture》2004,84(14):1908-1918
Whey protein isolate (WPI) was hydrolysed for 1 h using Alcalase, Protamex and Flavourzyme. Native WPI, hydrolysed WPI and two commercial WPI hydrolysates were subjected to fractionation by size exclusion chromatography. Antioxidant activity of WPI fractions was measured with a liposome‐oxidising system (50 µM FeCl3/0.1 µM ascorbate, pH 7.0). Lipid oxidation was measured as thiobarbituric acid‐reactive substances (TBARS). Gel electrophoresis and amino acid analysis were run to identify the peptide composition. The influence of amino acid composition on antioxidant activity was evaluated using multivariate analysis methods (correlation analysis, principal component analysis, multiple linear regression and discriminant analysis). TBARS assays indicated the presence of antioxidant activity in all protein fractions, including non‐hydrolysed WPI. For native and hydrolysed WPI samples the first fraction (> 45 kDa) showed a higher TBARS inhibition effect (24–27%) when compared with lower‐molecular‐weight fractions and hydrolysate mixtures. In contrast, for commercial WPI hydrolysates a higher inhibitory effect was found in most of the lower‐molecular‐weight fractions (30–55%). The ability of WPI fractions to delay lipid oxidation was found to be related to the prevalence of histidine and hydrophobic amino acids. Copyright © 2004 Society of Chemical Industry 相似文献
73.
A simple method has been devised for immobilization of acetylcholinesterase (AChE) covalent bonding to a multiwall carbon
nanotube (MWNT)-cross-linked cellulose acetate composite on a screen-printed carbon electrode (SPCE) and a sensitive and disposable
amperometric sensor for rapid determination of carbaryl pesticide is proposed. The immobilized enzyme was preserved on this
film because of the excellent biocompatibility and non-toxicity of cellulose acetate. Based on the inherent conductive properties
of the MWNT, the immobilized AChE had greater affinity for ATCl and excellent catalytic effect in the hydrolysis of ATCl.
MWNT improved the interface enzymatic hydrolysis reaction and increased the amperometric response of the sensor. Under optimum
conditions, the inhibition of carbaryl on AChE increased linearly with the increasing concentration of carbaryl in two ranges,
from 0.01 to 0.5 μg mL−1 and from 2 to 20 μg mL−1, with the correlation coefficients of 0.9985 and 0.9977, respectively. The detection limit was 0.004 μg mL−1 taken as the concentration equivalent to 10% decrease in signal. The sensor showed acceptable stability, accuracy and could
be fabricated in batches, thus it is economic and portable. This type of disposable enzyme-based amperometric sensor has extensive
application potential in environmental monitoring of pesticides. 相似文献
74.
Effect of Flour Quality, Ascorbic Acid, and DATEM on Dough Rheological Parameters and Hearth Loaves Characteristics 总被引:1,自引:0,他引:1
ABSTRACT: The effects of protein quality, protein content, ascorbic acid, diacetyl tartaric acid ester of monoglyc-erides (DATEM), and their interactions on dough rheology and hearth bread properties were studied by size-exclusion fast protein liquid chromatography, Kieffer Dough & Gluten Extensibility Rig, and small-scale baking of hearth loaves. The effect of protein content was either positive or negative on hearth loaf characteristics, form ratio, and area, depending on the amount of the largest glutenin polymers in the flour. Ascorbic acid brought out the potential in the wheat flour known as protein quality. Ascorbic acid and DATEM strengthened the doughs and improved hearth bread characteristics. 相似文献
75.
文章介绍了超精度的新型地震传感器DSU,它由微机电系统(MEMS)和兼有模拟电路和24位△—∑数字电路的混合器件(ASIC)组成,其失真度可达0.0032%,响应频带0~800Hz。DSU采集链使采集站、大线和检波器合为一体,既消除了电磁干扰和道间串音,又提高了采集数据的精度。DSU及DSU采集链的应用将为地震采集技术向超精度发展提供可靠保证。 相似文献
76.
文章依据FBG温度传感的原理以及LPG滤波技术,提出了一种新的光纤光栅温度报警模型.用MATLAB对系统所采用的方法进行了模拟,结果表明该系统能满足温度报警的要求. 相似文献
77.
Abdul S Chaudhry 《Journal of the science of food and agriculture》2007,87(14):2617-2624
BACKGROUND: Two factorial studies compared enzymic and in sacco methods to estimate degradation of ruminant foods. Enzyme degradation (in vitro = enzyme) was determined from the release of leucine‐equivalent amino acid (LA) crude protein (CP) from sunflower meal (SF), maize gluten meal (MG), distillers' dark grain (DG) and field beans (FB) after their separate incubations with Streptomyces griseus enzyme for 0–24 h. In sacco crude protein (CP) degradation of these foods was estimated during washing (0 h) and rumen incubations in fistulated cows for 2–24 h. The LA data were expressed as g LA per either kg of CP (LACP) or acid‐hydrolysable LA (HLA) of each food and compared with in sacco data. RESULTS: These methods showed comparable degradation with time (P < 0.01). The in sacco and HLA were greater than LACP for all foods except MG where in sacco value was either lower or equal to LACP depending upon the incubation time (P > 0.05 or P < 0.05). Conversely, HLA was significantly (P < 0.01) greater than LACP from 2 h onwards. At 0 h, in sacco values were significantly greater than those of enzyme for SF, DG and FB (P < 0.05) but not for MG. The foods differed significantly for degradation constants (a, b, c) in each method (P < 0.05). CONCLUSIONS: Despite variations between in sacco and enzyme estimates for different foods, the relationships between these estimates suggest that the HLA enzyme method has the potential to estimate food degradation. Copyright © 2007 Society of Chemical Industry 相似文献
78.
79.
We report here a non-enzymatic detecting electrode strip for fast monitoring of uric acid in human whole blood. A single-use amperometric uric acid sensor strip, incorporating a three-electrode configuration, has been fabricated on a polypropylene substrate using low cost screen-printing (thick-film) technology. Both the working and counter electrodes were prepared by screen-printing commercial carbon ink. The integration on the same support of pseudo-reference electrode was obtained by screen-printing a commercial silver ink and subsequent electrochemical pretreatment. Simply by placing a 20 μl human whole blood drop on the strip is enough for uric acid analysis by square-wave voltammetry. Real human whole blood samples were analyzed by this method and compared to the phosphotungstic acid clinical test procedure with satisfactory results. 相似文献
80.
低工作温度的氧化钨气敏材料 总被引:3,自引:0,他引:3
本文报道了一种可以在100℃左右工作的氢化钨气敏材料,它对氢气和一氧化氮有很高的灵敏度和良好的选择性,并可对空气中的一氧化氮进行探测 相似文献