Comparison in purity and antitumor effect of brand and generic paclitaxel against human ovarian cancer cells by an in vitro experimental model

Context: The purity and the therapeutic effectiveness of the generic paclitaxel have not yet been examined and compared to the original brand form. Objective: This study aimed to compare the in vitro purity and biological effects of original brand form (Taxol) and a generic drug of paclitaxel. Materials and Methods: Purity was determined by high-performance liquid chromatography analysis, cell viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay, cell proliferation by clonogenic assay, morphology by Liu's staining, and cell cycle distribution by DNA histogram. Results: Taxol and generic paclitaxel shared similar high-performance liquid chromatography profiles with a major peak at the same retention time and ultraviolet spectrum. Generic paclitaxel inhibited the cell viability to an extent greater than Taxol. By assessing the IC₅₀, generic paclitaxel also exhibited a greater inhibitory activity on clonogenicity of human ovarian adenocarcinoma SKOV-3 cells. Although both generic paclitaxel and Taxol arrested SKOV-3 and ES-2 cells at G2/M phase with concurrent development of hypoploid and polyploid cells, Taxol treatment exhibited markedly less extent of these changes. Observation of cellular morphology revealed a greater amount of mitotic catastrophe-like and apoptotic cells in generic paclitaxel-treated cells than Taxol-treated cells. Discussion and Conclusion: The results suggest that generic paclitaxel may possess a greater cell death inducing capacity and clonogenicity inhibitory activity against ovarian cancer cells than the original brand Taxol of the same purity. We conclude that this experimental model for assessing the difference between generic and brand name drugs might be considered as a reference while determining their interchangeability and could be easily established in a hospital-based laboratory.     

SGB630/220输送机电缆槽的改进

针对各煤矿用户的需要,对从两生产厂家购进的SGB630/220输送机电缆槽底板连接部位作了改进,使其电缆槽能互换使用。经用户使用结果表明,改进方案合理,具有良好的互换性和实用性。     

上海地区燃烧火焰稳定性与燃气互换性研究

通过配制不同比例,华白数和燃烧势各异的人工燃气,利用家用燃气热水器和家用灶具得到家用燃具的回火曲线,离焰曲线和不完全燃烧曲线,从而确定上海地区燃烧火焰的稳定性和燃气互换性区域。     

二甲醚替代石油液化气的可行性研究

二甲醚作为新型清洁能源,不仅可以解决我国油气短缺的问题,而且燃烧后的烟气中不含有对大气环境有污染的SO2等.本文对二甲醚替代石油液化气的可行性进行了研究.根据燃气互换性原理,将石油液化气燃具改造成适合二甲醚燃烧的燃具,并对燃烧效果进行了测试分析.结果表明,烧开相同质量、温度的水,二甲醚燃具所需时间及燃料消耗量均低于石油液化气燃具;另外在单位时间内,二甲醚的消耗量略高于石油液化气的消耗量,表明二甲醚燃烧速度比石油液化气高.二甲醚作为清洁能源,在燃烧温度、单位燃烧时间消耗量等方面都优于石油液化气.为二甲醚作为工业清洁能源提供了新思路和基础.     

关于燃气互换性与燃具适应性的探讨

燃气成分的变化将引起燃气燃烧特性的改变 ,从而引起燃具热负荷、一次空气系数、火焰结构等的变化 ,本文详细分析了燃气互换性及燃具适应性之间相互关联制约的作用。     

具有可互换性的虚拟信号发生器

对IVI(Interchangeable Virtual Instrument)技术规范的结构、特点及在软件中的应用进行了研究.通过虚拟仪器技术利用LabWindows/CVI工具开发了一套基于IVI驱动的虚拟信号发生器,设计的虚拟信号发生器可以兼容测控通过任何接口介入以及不同品牌的信号发生器,相对于传统仪器具有即插即测、兼容性好、可互换性强等特点.     

IVI.NET仪器驱动及其应用研究

IVI.NET是继IVI-C和IVI-COM之后的新一代IVI驱动标准,它继承了.NET的诸多优点并拓展了传统IVI的功能.研究了IVI.NET仪器驱动实现仪器互换的基本原理,分析了基于IVI.NET仪器驱动开发自动测试系统软件的优势,并以直流电源为例详细说明了开发和使用IVI.NET仪器驱动的方法和步骤.实践表明,IVI.NET仪器驱动在分布式的网络环境下开发,且使用高效,能够便捷地实现测试仪器的互换,对ATS软件开发人员具有一定的借鉴意义.     

上海多气源供气条件下天然气互换性问题

介绍了天然气质量的主要指标,针对上海三大规划气源的组分和物理特性,结合判断互换性的两个指标：华白数和燃烧势,对上海多气源条件下的天然气互换性问题进行了探讨,提出了部分对策和建议。     

Al_2O_3-WO_3材料的激光合成研究

采用大功率ＣＯ２激光器合成出了Ａｌ２Ｏ３－ＷＯ３陶瓷材料。测量其阻－温特性表明合成样品具有线性的ＮＴＣ热敏电阻特性，微观分析结果证明激光合成样品由Ａｌ２Ｏ３、ＷＯ３、Ａｌ２（ＷＯ４）３、ＡｌｘＷＯ３四相组成，而起导电作用的是ＡｌｘＷＯ３这一非平衡反应产物。常规烧结工艺合成Ａｌ２Ｏ３－ＷＯ３陶瓷材料所得试样没有线性的ＮＴＣ热敏电阻特性。     

Improvement in metrological provision for processing technology of precision components in the nanometer range

Studies are carried out for contact and contactless methods of measurement and monitoring plane-parallel gauge blocks and precision components in the nanometer range in order to provide uniformity of linear parameter measurements. __________ Translated from Izmeritel’naya Tekhnika, No. 8, pp. 22–27, August, 2007.