泰和乌骨鸡鸡胚抗疲劳作用研究

研究泰和乌骨鸡鸡胚对小鼠抗疲劳作用。200只昆明种雄性小白鼠预饲1w后,随机分为十组,其中实验组为6、10、14日龄的鸡胚分别设4.8、2.4、1.2g/kg·d三个剂量组给小鼠灌胃,对照组灌胃生理盐水,0.4ml/只,连续30d后,测定小白鼠负重游泳时间、肝糖原(HG)、乳酸脱氢酶(LDH)、血尿素氮(BUN)、血乳酸(LD)等指标。结果显示鸡胚能够延长小鼠游泳时间,提高运动后LDH活力及HG的储备量;降低血LD和BUN的含量,提高小鼠运动耐力。10日龄鸡胚功效较好,表明泰和乌骨鸡鸡胚具有增强机体抗疲劳能力。     

鸸鹋蛋壳粉对应激小鼠性功能和幼龄小鼠精子活性的影响

目的:观察国产鸸鹋蛋壳粉对应激小鼠性功能及幼龄小鼠精子活性的影响.方法:以重复电刺激复制应激小鼠性行为障碍模型,观察雄性小鼠的交配能力.给正常幼龄雄性小鼠连续服药21d后检测其精子密度和精子活动率.结果:鸸鹋蛋壳粉对电刺激应激诱发的雄性小鼠性功能障碍有促进恢复作用,能增加交配次数,缩短交配潜伏时间,并能提高未成年雄性小鼠的精子活动率.结论:国产鸸鹋蛋壳粉具有增强雄性小鼠性功能,提高小鼠精子活性的作用.     

小鼠乳腺中FGF7及其受体的表达与作用

为了从蛋白水平研究成纤维细胞生长因子7（FGF7）及其受体（KGFR）在哺乳动物乳腺发育、泌乳及退化过程中表达定位的动态变化,揭示FGF7及其受体表达变化与乳腺发育及泌乳功能的关系,本研究利用激光扫描共聚焦显微系统对昆明鼠乳腺中FGF7及其受体的表达进行了免疫荧光检测,利用体外培养不同发育时期的小鼠乳腺组织研究了FGF7在乳腺中的作用。结果表明：FGF7主要定位于乳腺导管上皮细胞以及腺泡上皮细胞外周,同时在基质中有弱的表达。KGFR主要出现在导管上皮细胞以及腺泡上皮细胞上,在乳腺发育的个别时期细胞外基质中有弱表达。FGF7在退化4d达到最高值;KGFR在泌乳4d达到最高值。除青春期和妊娠期外,添加FGF7均能显著诱导乳腺上皮细胞产生KGFR。FGF7能促进小鼠乳腺上皮细胞的增殖分化,降低乳腺上皮细胞的细胞凋亡。     

枸杞多糖对小鼠肠道上皮内淋巴细胞和杯状细胞数量、分布及对IL-2水平影响

为了研究枸杞多糖(LBP)对肠黏膜屏障结构的影响,给10只C57BL/J健康小鼠连续灌服枸杞多糖7d后,显微镜观察小肠上皮内淋巴细胞(IEL)、杯状细胞(GC)数量和分布变化及测定肠黏膜白细胞介素-2(interleukin-2,IL-2)水平。结果表明,应用枸杞多糖小鼠的肠黏膜IEL数量和GC的数量明显增加,且有向肠内层移动趋势,同时肠黏膜IL-2水平升高。证实枸杞多糖在一定程度上可促进小鼠小肠黏膜屏障结构趋于完整并提高其免疫功能。     

The use of ballistic movement as an additional method to assess performance of computer mice

This study investigates an additional method for independently evaluating performance of speed and accuracy of computer mice, based on five parameters of the initial submovement when aiming at a target. This first submovement is known to be carried out in a ballistic manner and hence the Fitts-type aiming movement may be studied by means of the established relationships for ballistic movement. To test the ballistic movement method, eight participants used four different computer mice to execute ballistic movements and Fitts-type aiming movements. Regression tests showed that the Fitts movement time was well correlated with the time and stopping-variable error of ballistic movement, implying that measuring ballistic movement parameters can predict the performance of Fitts-type aiming movements. More importantly, the use of ballistic movement method allows independent assessments of the speed and accuracy, providing additional information for input devices assessment and designs.Relevance to industryThe study illustrates the close correspondence between input device evaluation using the proposed ballistic movement method and Fitts' law. The ballistic movement method allows independent measures of speed and four types of accuracy, which should benefit input devices assessment and designs.     

小白鼠生物样本中铂的测定方法研究

应用石墨炉原子吸收光谱法测定小白鼠生物样本中的铂含量进行了研究。通过二步灰化法与塞曼背景扣除法相结合，较好地解决了生物样本中的背景干扰问题，测定尿样的相对标准偏差为3．00％、肾皮质部消化液相对标准偏差为5．26％、血清相对标准偏差为9．42％，简化了测定程序，建立了对小白鼠尿、血清、骨皮质部消化液等生物样本均有较好适应性的痕量铂的测定方法。     

真实感虚拟人头发动态仿真研究

针对头发的不可伸展性和数量过大造成的头发仿真时性能低效、真实感不高问题,提出一种新的头发动态仿真方法。该方法首先利用弹簧模型对单根头发进行建模,然后采用DFTL(Dynamic Follow The Leader)算法进行变形计算,并基于FTL(Static Follow The Leader)算法对头发进行快速插值,最后运用Deep Opacity Mapping技术对头发进行渲染,增强虚拟人头发的真实感。同时,对头发与头部模型、头发与头发进行碰撞检测与响应。实验结果表明,该方法生成的头发逼真自然,而且在大量渲染时保持了较高的稳定性。     

黄腐酸对小鼠肝酶活性的影响

观察小鼠饲料添加黄腐酸饲养19周后肝酶组织的化学变化。结果显示:试验组与对照组相比,肝细胞内琥珀酸脱氢酶、单胺氧化酶和肝巨噬细胞内酸性磷酸酶的活性均有不同程度的增强。表明黄腐酸在促进肝细胞的物质代谢和增强肝生物转化功能等方面有一定的作用。     

Quantitative DNA measurements in an instrument combining scanning electron microscopy and light microscopy

An instrument for combined scanning electron microscopy (SEM) and light microscopy (LM) to which a photometer unit is attached is described. A special stage in the vacuum chamber of a scanning electron microscope incorporates light microscope optics (objective and condenser) designed for transmission and epi-illumination fluorescence LM. An optical bridge connects these optics to a light microscope, without objective and condenser. The possibility of performing quantitative DNA measurements in this combined microscope (the LM/SEM) was tested using preparations of either chicken erythrocytes, human lymphocytes, or mouse liver cells. The cells were fixed, brought on a cover-glass, quantitatively stained for DNA, dehydrated, and critical point dried (CPD). After mounting the cells were coated with gold. The specimens were brought into the vacuum chamber of the combined microscope and individual cells were studied with SEM and LM. Simultaneously DNA measurements were performed by means of the photometer unit attached to the microscope. It is shown in this study that DNA measurements of cells in the combined microscope give similar results when compared to DNA measurements of embedded cells performed with a conventional fluorescence microscope. Furthermore, it is shown that although the gold layer covering the LM/SEM specimens weakens the fluorescence signal, it does not interfere with the DNA measurements.     

Three-dimensional motion tracking for high-resolution optical microscopy, in vivo

When conducting optical imaging experiments, in vivo, the signal to noise ratio and effective spatial and temporal resolution is fundamentally limited by physiological motion of the tissue. A three-dimensional (3D) motion tracking scheme, using a multiphoton excitation microscope with a resonant galvanometer, (512 × 512 pixels at 33 frames s(-1)) is described to overcome physiological motion, in vivo. The use of commercially available graphical processing units permitted the rapid 3D cross-correlation of sequential volumes to detect displacements and adjust tissue position to track motions in near real-time. Motion phantom tests maintained micron resolution with displacement velocities of up to 200 μm min(-1), well within the drift observed in many biological tissues under physiologically relevant conditions. In vivo experiments on mouse skeletal muscle using the capillary vasculature with luminal dye as a displacement reference revealed an effective and robust method of tracking tissue motion to enable (1) signal averaging over time without compromising resolution, and (2) tracking of cellular regions during a physiological perturbation.