3D无线射频鼠标的设计与实现

一种3D无线鼠标设计的新思路,以凌阳单片机为核心控制器,通过MEMS加速度传感器和触控模块,感知鼠标的移动动作,同时,通过无线射频完成无线通信,从而实现鼠标功能。使用过程中,鼠标不仅可以放于载体使用,而且可以在空中使用,增添了鼠标的操控方式,同时,大大增加了此无线鼠标的适用范围。     

垂直鼠标设计中的人机工程学应用分析

以多彩科技生产的垂直鼠标为研究对象,通过与传统平放鼠标的人机设计对比分析,论述了由于鼠标革命的产物——垂直鼠标的熨斗形外观设计及右侧面的按键巧妙布置,进而消除了传统平放鼠标人机设计缺陷造成使用者手臂长期不适的状况,总结了垂直鼠标在人机设计中的独特之处,为设计师在产品设计中换位思考提供了新的思路和方法。     

羊栖菜多糖对小鼠抗疲劳作用的研究

研究羊栖菜多糖对小鼠的抗疲劳作用,对于开发利用羊栖菜资源具有重要意义。通过对小鼠灌胃不同剂量的羊栖菜多糖(50、150mg/mL·d),经15d后测定小鼠体重、负重游泳力竭时间、超氧化物歧化酶(SOD)、丙二醛(MDA)、肝糖原、肌乳酸及血清尿素的含量,研究羊栖菜多糖抗疲劳作用机理。结果表明,羊栖菜多糖对小鼠体重增长无明显影响(p>0.05),但能明显延长小鼠负重游泳时间(p<0.01),增强小鼠血清SOD活性(p<0.01),提高肝糖原含量(p<0.01),降低血清MDA(p<0.05)、肌乳酸(p<0.01、0.05)及血清尿素(p<0.01)的含量。说明羊栖菜多糖具有抗疲劳的作用。      

不同发酵天数的芝芪菌质对免疫抑制小鼠免疫功能的影响

为筛选较佳的芝芪菌质发酵时间,本实验通过小鼠碳粒廓清实验,鸡红细胞吞噬功能实验,小鼠血清溶血素、Ig M和Ig G的含量检测,以及小鼠脾淋巴细胞增殖实验,考察了不同发酵天数的芝芪菌质对免疫抑制小鼠非特异性和特异性免疫功能的影响。结果表明:发酵25、30 d的芝芪菌质具有显著的免疫增强功效(p<0.05,0.01;0.05),提示可有效增强免疫功能低下人群的机体免疫力。      

基于SolidWorks的鼠标壳注射模设计及模流分析

通过对鼠标壳制件进行工艺分析及注射模的模流分析,最终完成了注射模的结构设计。具体介绍了应用MoldFlow Plastics Insight（MPI）件进行充模、冷却、翘曲等方面的分析过程及应用三雏CAD软件SolidWorks进行模具设计的具体过程。     

Alteration in the Synaptic and Extrasynaptic Organization of AMPA Receptors in the Hippocampus of P301S Tau Transgenic Mice

Tau pathology is a hallmark of Alzheimer’s disease (AD) and other tauopathies, but how pathological tau accumulation alters the glutamate receptor dynamics driving synaptic dysfunction is unclear. Here, we determined the impact of tau pathology on AMPAR expression, density, and subcellular distribution in the hippocampus of P301S mice using immunoblot, histoblot, and quantitative SDS-digested freeze-fracture replica labeling (SDS-FRL). Histoblot and immunoblot showed differential regulation of GluA1 and GluA2 in the hippocampus of P301S mice. The GluA2 subunit was downregulated in the hippocampus at 3 months while both GluA1 and GluA2 subunits were downregulated at 10 months. However, the total amount of GluA1-4 was similar in P301S mice and in age-matched wild-type mice. Using quantitative SDS-FRL, we unraveled the molecular organization of GluA1-4 in various synaptic connections at a high spatial resolution on pyramidal cell spines and interneuron dendrites in the CA1 field of the hippocampus in 10-month-old P301S mice. The labeling density for GluA1-4 in the excitatory synapses established on spines was significantly reduced in P301S mice, compared to age-matched wild-type mice, in the strata radiatum and lacunosum-moleculare but unaltered in the stratum oriens. The density of synaptic GluA1-4 established on interneuron dendrites was significantly reduced in P301S mice in the three strata. The labeling density for GluA1-4 at extrasynaptic sites was significantly reduced in several postsynaptic compartments of CA1 pyramidal cells and interneurons in the three dendritic layers in P301S mice. Our data demonstrate that the progressive accumulation of phospho-tau is associated with alteration of AMPARs on the surface of different neuron types, including synaptic and extrasynaptic membranes, leading to a decline in the trafficking and synaptic transmission, thereby likely contributing to the pathological events taking place in AD.     

混合菌种发酵酸马乳的降血脂功能研究

为研究混合菌种发酵酸马乳的降血脂作用,采用混合菌种发酵酸马乳对小鼠进行实验,研究其对高脂模型小鼠体重、血脂水平、脏器指数和动脉硬化（AI）指数的影响。结果表明,混合菌种发酵酸马乳中、高剂量能够显著降低高血脂症小鼠TC、TG、LDL-C、AI水平（p<0.05）,显著升高HLD-C水平（p<0.05）,并且能降低小鼠体重和肝脏指数,说明混合菌种发酵酸马乳对实验性高脂血症小鼠具有降低血脂的作用,对高脂血症及动脉粥样硬化具有一定的预防作用。      

Structural Characteristics of the 5′-Terminal Region of Mouse p53 mRNA and Identification of Proteins That Bind to This mRNA Region

A mouse model has often been used in studies of p53 gene expression. Detailed interpretation of functional studies is, however, hampered by insufficient knowledge of the impact of mouse p53 mRNA’s structure and its interactions with proteins in the translation process. In particular, the 5′-terminal region of mouse p53 mRNA is an important region which takes part in the regulation of the synthesis of p53 protein and its N-truncated isoform Δ41p53. In this work, the spatial folding of the 5′-terminal region of mouse p53 mRNA and its selected sub-fragments was proposed based on the results of the SAXS method and the RNAComposer program. Subsequently, RNA-assisted affinity chromatography was used to identify proteins present in mouse fibroblast cell lysates that are able to bind the RNA oligomer, which corresponds to the 5′-terminal region of mouse p53 mRNA. Possible sites to which the selected, identified proteins can bind were proposed. Interestingly, most of these binding sites coincide with the sites determined as accessible to hybridization of complementary oligonucleotides. Finally, the high binding affinity of hnRNP K and PCBP2 to the 5′-terminal region of mouse p53 mRNA was confirmed and their possible binding sites were proposed.     

BXSB 狼疮小鼠肾组织TGF-β1异常表达及甲泼尼龙干预作用

目的: 研究转化生长因子-β₁ (TGF-β₁) 在BXSB小鼠狼疮肾炎(LN) 中的作用及甲泼尼龙(MPS) 是否可通过影响TGF-β₁ 表达而改善LN 。方法: 用逆转录-聚合酶链反应技术(RT-PCR) 和免疫组织化学方法检测BXSB 狼疮小鼠及BALB /c 小鼠肾组织TGF-β1 mRNA 及蛋白的表达情况, 并用甲泼尼龙对BXSB小鼠进行体内干预, 观察甲泼尼龙对BXSB 小鼠肾脏TGF-β₁ 表达的影响及各组小鼠24 h 尿蛋白量的变化。结果: RT-PCR 检测到BXSB 小鼠肾组织TGF-β₁mRNA 表达明显高于BALB /c 小鼠(P <0.01); 其中, BXSB 小鼠治疗组肾组织TGF-β₁mRNA 表达量显著低于对照组(P <0.01) 。BXSB 小鼠治疗组24 h尿蛋白量明显低于对照组(P <0.01) 。免疫组织化学检测示TGF-β₁ 表达于BXSB 小鼠肾小球系膜细胞、肾小管上皮细胞、血管内皮细胞、肾间质及肾小球浸润的炎症细胞胞质内, 其中BXSB 小鼠治疗组较对照组表达明显减弱, 而BALB /c 小鼠仅在少数肾小管上皮细胞有微弱表达, 且图像半定量分析显示各组有显著性差异(P <0.01) 。结论: TGF-β₁ 可能参与LN 发病;甲泼尼龙可能通过抑制肾组织TGF-β₁ 的表达来改善LN。