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41.
    
Plants have various self-defense mechanisms against biotic attacks, involving both physical and chemical barriers. Physical barriers include spines, trichomes, and cuticle layers, whereas chemical barriers include secondary metabolites (SMs) and volatile organic compounds (VOCs). Complex interactions between plants and herbivores occur. Plant responses to insect herbivory begin with the perception of physical stimuli, chemical compounds (orally secreted by insects and herbivore-induced VOCs) during feeding. Plant cell membranes then generate ion fluxes that create differences in plasma membrane potential (Vm), which provokes the initiation of signal transduction, the activation of various hormones (e.g., jasmonic acid, salicylic acid, and ethylene), and the release of VOCs and SMs. This review of recent studies of plant–herbivore–infection interactions focuses on early and late plant responses, including physical barriers, signal transduction, SM production as well as epigenetic regulation, and phytohormone responses.  相似文献   
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A novel method for the selective catalytic N-dealkylation of drug molecules on a nanoporous gold (NPG) catalyst producing valuable N-dealkylated metabolites and intermediates is described. Drug metabolites are important chemical entities at every stage of drug discovery and development, from exploratory discovery to clinical development, providing the safety profiles and the ADME (adsorption, distribution, metabolism, and elimination) of new drug candidates. Synthesis was carried out in aqueous solution at 80 °C using air (oxygen source) as oxidant, in single step with good isolated yields. Different examples examined in this study showed that aerobic catalytic N-dealkylation of drug molecules on NPG has a broad scope supporting N-deethylation, N-deisopropylation and N-demethylation, converting either 3° amines to 2° amines, or 2° amines to 1° amines.  相似文献   
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Microgravity, one of the conditions faced by astronauts during spaceflights, triggers brain adaptive responses that could have noxious consequences on behaviors. Although monoaminergic systems, which include noradrenaline (NA), dopamine (DA), and serotonin (5-HT), are widespread neuromodulatory systems involved in adaptive behaviors, the influence of microgravity on these systems is poorly documented. Using a model of simulated microgravity (SMG) during a short period in Long Evans male rats, we studied the distribution of monoamines in thirty brain regions belonging to vegetative, mood, motor, and cognitive networks. SMG modified NA and/or DA tissue contents along some brain regions belonging to the vestibular/motor systems (inferior olive, red nucleus, cerebellum, somatosensorily cortex, substantia nigra, and shell of the nucleus accumbens). DA and 5-HT contents were reduced in the prelimbic cortex, the only brain area exhibiting changes for 5-HT content. However, the number of correlations of one index of the 5-HT metabolism (ratio of metabolite and 5-HT) alone or in interaction with the DA metabolism was dramatically increased between brain regions. It is suggested that SMG, by mobilizing vestibular/motor systems, promotes in these systems early, restricted changes of NA and DA functions that are associated with a high reorganization of monoaminergic systems, notably 5-HT.  相似文献   
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Secondary metabolites are structurally diverse natural products (NPs) and have been widely used for medical applications. Developing new tools to enrich NPs can be a promising solution to isolate novel NPs from the native and complex samples. Here, we developed native and deuterated chemoselective labeling probes to target phenol-containing glycopeptides by the ene-type labeling used in proteomic research. The clickable azido-linker was included for further biotin functionalization to facilitate the enrichment of labeled substrates. Afterward, our chemoselective method, in conjunction with LC-MS and MSn analysis, was demonstrated in bacterial cultures. A vancomycin-related phenol-containing glycopeptide was labeled and characterized by our labeling strategy, showing its potential in glycopeptide discovery in complex environments.  相似文献   
47.
Balances of major nutrients such as nitrogen (N), phosphorus (P), and potassium (K) in small-scale farming systems are of critical importance to nutrient management and sustainable agricultural development. Mass balances of N, P, and K and some of their influencing factors were studied for two years from July 2003 to July 2005 on small-scale vegetable-farming systems in two contrasting peri-urban areas (Nanjing and Wuxi) of the Yangtze river delta region of China. This balance approach considered organic fertilizer inputs (cow manure, pig manure, and human biosolids), inorganic fertilizer inputs (urea, composite fertilizer, and phosphates), irrigation water, and atmospheric deposition; and considered outputs by vegetables. Input via organic fertilizers was significant for all element balances in the Nanjing area. Inorganic and organic fertilizer, particularly inorganic fertilizer, contributed major nutrient inputs to the system in the Wuxi area. Compared with nutrient output by vegetables, there were significant surpluses of N and P on two vegetable farm systems. Furthermore, N surplus in the Nanjing area was higher than that in the Wuxi area with an inverse relationship to P surplus. In contrast, the general trend of K balances was negative on both sites; hence, the nutrient use efficiency was significantly lower for N and P than K. The nutrient imbalance may be attributed to the differences between fertilizer types and management modes driven by social economic status differences among farmer households. The large N and P net excess creates an environmental threat because of potential losses to ground or surface waters, whereas negative K balance creates soil fertility risks. The results highlight researchers’ and farmers’ need to develop rational fertilization technology to optimize nutrient management on vegetable farmlands to promote sustainable agricultural development in peri-urban areas.  相似文献   
48.
基于代谢组学方法研究低能离子束诱变重组菌株N6076与原始菌株Kh08在不同发酵时期的差异性胞外代谢产物,并通过网络药理学探讨了相关代谢通路.结果表明,不同时期两种菌株的胞外代谢产物存在明显差异,鉴定出VIP>1的15种代谢差异物,其中吲哚3-丙酸不仅作为共同的代谢产物,而且其差异性也最为显著.基于网络药理学的差异代谢...  相似文献   
49.
食源性沙门菌流行趋势及耐药性研究进展   总被引:1,自引:0,他引:1  
沙门菌病是主要的食源性疾病,构成了全球范围内主要的公共卫生问题,我国由沙门菌引起的食源性疾病也占首位.研究发现,肉制品可能是人类沙门菌病主要的传染源,且近年来在全球范围内,肉制品中耐药性沙门菌的检出率居高不下,这使临床医生治疗时可选择的药物受到限制.随着家禽肉类食品的日益全球化,沙门菌病的控制可能会面临更严峻的挑战,因...  相似文献   
50.
This study was performed to investigate blood metabolite, tumor necrosis factor-alpha, and hormone responses to intravenous administration of lipopolysaccharides (2 microg of endotoxin of Escherichia coli 026:B6/kg body weight at times of feeding) in veal calves orally supplemented with arginine (0.25 g/kg of body weight twice daily for 4 d; group GrA) compared with calves not supplemented with arginine (group GrC). Arginine supplementation alone caused a significant rise of plasma arginine, urea, and insulin concentrations, whereas glucagon concentrations tended to increase, but there were no significant group differences. Concentrations of triglycerides, NEFA, glucose, protein, albumin, growth hormone, insulin-like growth factor-I, 3.5.3'-triiodothyronine, and thyroxine were not affected by arginine supplementation. Lipopolysaccharide administration alone caused a rise of tumor necrosis-factor-a, lactate, and cortisol concentrations and concentrations of tumor necrosis-factor-a after 1 h, and of triglycerides and urea after 6 h were higher, whereas of glucose after 3 h were lower in GrA than in GrC. Concentrations of NEFA, glucose, protein, albumin, insulin, growth hormone, insulin-like growth factor-I, 3.5.3'-triiodothyronine, and thyroxine were not affected by lipopolysaccharide administration. In conclusion, arginine supplementation had selective effects on plasma metabolites and hormones, but barely modified lipopolysaccharide effects. Effects of lipopolysaccharides in the postprandial state were different from what is usually seen in the fasted state.  相似文献   
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