基于MEMS微电极芯片的电穿孔系统

报道了一种基于微加工金电极的细胞电穿孔芯片及一套完整的细胞电穿孔系统。该系统能够在多种细胞系中实现高效细胞电穿孔(对典型HEK-293A(人胚胎肾细胞)细胞的穿孔效率高于90%,3T3-L1(小鼠胚胎成纤维细胞)的电穿孔效率高达80%)。并且具有手动模式和自动模式。同时,由于基于独特的电极设计,其所需电压也远低于现有设备(典型工作电压为60 V),成本更低,操作更安全。     

用于麦克风阵列的阵元筛选方法研究

在使用TDOA算法进行被动声定向时,为精确获取麦克风阵列阵元之间接收声源信号的时间差,进而计算目标的方位角,麦克风阵元之间必须保持较高的相位一致性。而从论文与专利检索来看,目前还没有有效方法筛选出符合要求的麦克风阵元。针对此问题,本文基于麦克风阵列阵元相位一致性的指标,设计出麦克风阵元筛选系统,通过实测数据分析表明:一致性较高的麦克风更适合组建麦克风阵列。     

多功能USB 2.0集线器的设计与实现

在许多数码产品应用中,存在计算机USB接口不足,使用各种数码卡时需配备专用读卡器的问题。为解决这些问题,本文使用FE1.1S和AU6437芯片设计一种多功能USB集线器,可提供一个SD卡和Micro SD卡接口,以及两个标准USB 2.0扩展口。经测试,本设计硬件功能完善,性能稳定,符合USB 2.0标准,为多功能高速读卡器的设计提供了良好的技术参考和依据。     

紧扣内存变量关系巧解矩阵类问题

C语言学习过程中,不少初学者不能建立思维的模型,无法将形象可行的矩阵运算、旋转事件抽象化成算法语言。讲解了指导学生掌握矩阵操作算法的推导方法。     

A Novel Artificially Humanized Anti-Cripto-1 Antibody Suppressing Cancer Cell Growth

Cripto-1 is a member of the EGF-CFC/FRL1/Cryptic family and is involved in embryonic development and carcinogenesis. We designed a novel anti-Cripto-1 artificial antibody and assessed the recognition to the antigen and the potential to suppress the growth of cancer stem cells. First, single chain antibody clones were isolated by bio-panning with the affinity to recombinant Cripto-1 protein from our original phage-display library. Then, the variable regions of heavy chain VH and light chain VL in each clone were fused to constant regions of heavy chain CH and light chain CL regions respectively. These fused genes were expressed in ExpiCHO-S cells to produce artificial humanized antibodies against Cripto-1. After evaluation of the expression levels, one clone was selected and the anti-Cripto-1 antibody was produced and purified. The purified antibody showed affinity to recombinant Cripto-1 at 1.1 pmol and immunoreactivity to cancer tissues and cell lines. The antibody was available to detect the immunoreactivity in tissue microarrays of malignant tumors as well as in Cripto-1 overexpressing cells. Simultaneously, the antibody exhibited the potential to suppress the growth of human colon cancer derived GEO cells overexpressing Cripto-1 with IC₅₀ at approximately 110 nM. The artificially humanized antibody is proposed to be a good candidate to target cancer cells overexpressing Cripto-1.     

轧机主传动安全销弹塑性有限元分析

本文针对轧机主传动系统的安全销在破坏前已产生了塑性变形,采用三维弹塑性有限元对安全销进行应力分析,模拟出材料的应力-应变非线性曲线,并运用牛顿-拉普森平衡迭代法根据模拟出的应力-应变非线性曲线对安全销进行应力计算,通过多次试算得出其能承受的最大载荷,并提出了改进意见.