Polyphasic characterization of indigenous lactobacilli and lactococci from PDO Canestrato Pugliese cheese

A polyphasic approach, involving both genotypic and phenotypic analyses was used to characterize 33 isolates of lactic acid bacteria from the raw milk Protected Designation of Origin (PDO) Canestrato Pugliese cheese, in order to select candidate strains that can be used as autochthonous starter cultures in the dairy industry. Randomly amplified polymorphic DNA (RAPD) analysis and clustering by the unweighted pair-group method with arithmetic averages (UPGMA) were used to evaluate the genotypic diversity, while phenotypic characterization was performed through miniaturized assays and traditional biochemical tests. Technological properties of major interest for cheese-making (acidification, tendency to lysis, proteolytic and peptidase activity) were also evaluated, and selected subset of data was statistically examined by the one-way ANOVA and the Tukey-Kramer HSD test, to highlight strain-specific and species-specific differences among the isolates. A high degree of diversity appeared in the phenotypic and technological traits in opposition to a relatively low genotypic diversity. Although none of the isolates showed the best performances in all the activities, an appropriate mixture of strains could be selected for providing an efficacious autochthonous starter culture.     

软体沉排在护岸工程中的应用

软件沉排是既安全稳定又经济快捷的设计方案和施工方法之一，软体沉排适用于河床主流，仅靠岸边，岸坡较陡，施工难度较大的地形，通过介绍软梯沉排的基本结构和在护岸工程中的作用，阐述了软体沉排具有整体性好，适应性强，施工工艺简单，工程效果好等特点。     

建福大厦结构设计

本文概况介绍了建福大厦的桩基设计 ,支护体系 ,上部结构计算。着重阐述了框架 -筒体结构的剪力墙布置 ,电算结果分析处理等     

Effect of temperature on fermentative hydrogen production by mixed cultures

Effect of temperatures ranging from 20 °C to 55 °C on fermentative hydrogen production by mixed cultures was investigated in batch tests. The experimental results showed that, at initial pH 7.0, during the fermentative hydrogen production using glucose as substrate, the substrate degradation efficiency and hydrogen production potential increased with increasing temperatures from 20 °C to 40 °C. The maximal substrate degradation efficiency was 98.1%, the maximal hydrogen production potential was 269.9 mL, the maximal hydrogen yield was 275.1 mL/g glucose and the shortest lag time was 7.0 h. The temperature for fermentative hydrogen production by mixed cultures was optimized to be 40 °C. The expanded Ratkowsky models could be used to describe the effect of temperatures on the hydrogen production potential, maximum hydrogen production rate and the lag time during fermentative hydrogen production.     

Anisotropic Crystalline Protein Nanolayers as Multi‐Functional Biointerface for Patterned Co‐Cultures of Adherent and Non‐Adherent Cells in Microfluidic Devices

The spatial arrangement of cells in their microenvironment is known to significantly influence cellular behavior, thus making the control of cellular organization an important parameter of in vitro co‐culture models. However, recent advances in micropatterning co‐culture methods within biochips do not address the simultaneous cultivation of anchorage‐dependent and non‐adherent cells. To address this methodological gap we combine S‐layer technology with microfluidics to pattern co‐cultures to study the cell‐to‐cell and cell‐to‐surface interactions under physiologically relevant conditions. We exploit the unique self‐assembly properties of SbpA and SbsB S‐layers to create an anisotropic protein nanobiointerface on‐chip with spatially‐defined cytophilic (adhesive) and cytophobic (repulsive) properties. While microfluidics control physical parameters such as shear force and flow velocities, our anisotropic protein nanobiointerface regulates the biological aspects of the co‐culture method including biocompatibility, biostability, and affinity to non‐adherent cells. The reliability and reproducibility of our microfluidic co‐culture strategy based on laminar flow patterned protein nanolayers is envisioned to advance in vitro models for biomedical research.     

筏板基础质量控制的探讨

随着建筑业的发展,伐板基础应用增多。地基基础的施工质量对建筑物的整体质量产生主要影响。论文主要通过结合工程实例,主要介绍从基坑开挖到伐板基础施工及浇筑后的养护措施等与伐板基础相关的施工阶段,探讨梁板式筏板基础的施工质量控制工作,并对施工过程中遇到的问题提出解决方法。     

船载柴油机浮筏隔振系统的主动控制策略研究

针对柴油机等船载动力机械的隔振降噪问题，采用子结构导纳方法建立了船载柴油机浮筏隔振系统的主被动联合控制模型，实现了基于导纳矩阵的浮筏隔振系统的主动控制和被动控制的统一数学描述。给出了浮筏隔振系统的机器控制、浮筏控制和全控制等各种控制方法的概念，并阐释了它们之间的联系。最后以传递到基础的功率流为价值函数，对比研究了不同控制策略下各种控制形式的主动浮筏隔振系统的功率流传递特性，并给出了主动浮筏系统隔振设计的基本准则。     

某高层建筑基础穿越防空洞处理措施

在重庆市旧城改造中经常会遇到抗日战争期间遗留下的废弃防空洞,防空洞的处理需谨慎,若处理不当会造成资源上的浪费或不能保证建筑物的结构安全。本文结合工程实践,从防空洞的复测、废弃防空洞的施工处理、局部开挖封仓回填工艺等几个方面对此进行了详细的阐述。     

复杂桩基筏板冲切线的选取

在高层建筑基础设计中,复杂桩基筏板的冲切线选取较无桩筏板更难以确定,可能的冲切线有很多条,但最不利的只有一条。对某超高层建筑的复杂桩基筏板共选取了四组不同形式的冲切线进行试算,得出了三道冲切线在这四组冲切线形式下的抗冲切反力,并进行了冲切计算的对比分析。结果表明,随竖向构件的直转角的冲切线(第一组)为最不利的初步结论,同时也指出各组中不利的冲切线数值差异不大,宜进行多种情况下多条冲切线的试算。在设计中,对冲切承载力预留一定的安全储备,同时配置一定的构造钢筋是有必要的。     

地下室混凝土结构施工的保温养护分析

以某大厦地下室混凝土浇筑温控施工为例,阐述大体积混凝土筏基和长墙在施工方案阶段应做的温度控制试算分析工作。厚2.2 m的筏基在日平均温度为22℃时进行施工,采用混凝土表面贮水蓄热保温保湿养护措施是可行的,可使混凝土内、外温差有效控制在25℃以下,不会产生温度裂缝。而混凝土长墙带模保温养护、合理留置后浇带间距,是墙体预防...