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91.
采用量子化学密度泛函理论(DFT)研究贻贝粘附单元DOPA(3,4-二羟基苯丙氨酸)的结构与性质,得分子的几何构型、原子电荷分布、反应活性及热力学等参数,表明:DOPA苯环易与HClO(次氯酸)发生亲电取代反应(1),生成3-氯4,5-二羟基苯丙氨酸,阻碍生成贻贝内超强粘附单元DOPA二联体,降低粘附蛋白间粘性;DOPA侧链易与HClO发生亲电亲核反应(2),促使DOPA侧链的断裂,降低粘附蛋白内粘性;在相同温度下,反应(1)和反应(2)的△G<0,且△G(1)<△G(2),反应(1)较易发生. 相似文献
92.
Heikki Srkel Jari Takatalo Patrick May Mikko Laakso Gte Nyman 《International journal of human-computer studies》2009,67(9):787-799
Human movement in virtual environments (VEs) is a largely unstudied area, and there are no well-established methods of measuring it in VEs. Consequently, it is unclear how movement affects the experiential side of VEs. We introduce a novel method of measuring and modelling human movement. A specific information entropy-based modelling method enabled us to identify different movement patterns and analyse the experiential components related to them. The data was collected by registering the movement patterns of 68 participants who were in a virtual house doing a search task. The experiential side of the VE was measured with the Experimental Virtual Environment Questionnaire (EVEQ). Four movement patterns were identified. In addition, fluent movement in VEs was related to a high sense of presence. Moreover, the participants who moved fluently in the environment assessed their skills high. The results show how movement is related the way in which people experience the VE. The movement analysis method introduced here is applicable to other related research areas as well. 相似文献
93.
The Amazon rainforest is one of the world's greatest natural wonders and holds great importance and significance for the world's environmental balance. Around 60% of the Amazon rainforest is located in the Brazilian territory. The two biggest states of the Amazon region are Amazonas (the upper Amazon) and Pará (the lower Amazon), which together account for around 73% of the Brazilian Legal Amazon, and are the only states that are serviced by international airports in Brazil's north region. The purpose of this paper is to model and forecast sustainable international tourism demand for the states of Amazonas, Pará, and the aggregate of the two states. By sustainable tourism is meant a distinctive type of tourism that has relatively low environmental and cultural impacts. Economic progress brought about by illegal wood extraction and commercial agriculture has destroyed large areas of the Amazon rainforest. The sustainable tourism industry has the potential to contribute to the economic development of the Amazon region without destroying the rainforest. The paper presents unit root tests for monthly and annual data, estimates alternative time series models and conditional volatility models of the shocks to international tourist arrivals, and provides forecasts for 2006 and 2007. 相似文献
94.
95.
Numeric and curve parameters for freeform surface feature models 总被引:1,自引:0,他引:1
The success of feature modelling systems depends to a large extent on offering user-friendly ways for specifying and varying feature models. In this paper, several new facilities are introduced for this.Numeric and curve parameters are presented for specification and variation of freeform surface feature models. Several properties of the parameters can be defined, such as the direction of evaluation for a curve parameter. In addition to parameters present in features, other parameters can be added to a feature model, including intersection curves between features.To provide these facilities, a new three-level structure is introduced, which includes a partially evaluated model that can capture new generic model entities, including feature intersection curves. The work has been implemented in a prototype surface feature modelling system with functionality for feature class definition, feature model creation, and advanced feature model variation through the new types of parameters. 相似文献
96.
97.
利用生物活性物质高效、专一的催化特性,通过生化反应中生成的复合物对包层折射率和半径的直接调制,提出了基于长周期光纤光栅的、可用于蛋白质等生物物质鉴别与分析的传感方案,并用数值计算方法对检测中所生成复合物薄膜层的折射率和厚度变化对耦合波长偏移和衰减的影响进行了仿真研究,为新型高灵敏度生物传感器的研发提供了理论依据。 相似文献
98.
P. Bison C. Ferrari E. Pagello L. Stocchiero 《Journal of Intelligent and Robotic Systems》1995,13(1):45-74
This paper presents the core of a software system able to determine a good grasp configuration on 3D objects for a three-fingered hand. The grasp planning problem has been studied considering both the constraints due to the stability and accessibility conditions, and the ones related to functionality. Physical, geometrical, spatial and task-related knowledge for solving the grasp planning problem have been properly modelled to support a heuristic-based reasoning process. A series of heuristic rules and geometric tests are used to scan the solution space, searching for a good grasp. In fact, when considering the three-dimensional case, a purely analytical and exhaustive approach appears too complex because of the dimension of the search space. This approach results in an incremental and modular model of grasp reasoning, that has been implemented using the Flex expert system shell. This work has been developed and demonstrated within the Esprit 2 project CIM-PLATO No. 2202. 相似文献
99.
目的 探究天然肌质钙结合蛋白(sarcoplasmic calcium binding protein, SCP)的可替代物,为蟹类过敏原的检测提供基础材料,本研究首次利用毕赤酵母(Pichia pastoris, P. pastoris)高效表达表达三疣梭子蟹(Portunus trituberculatus)重要过敏原SCP,并检验其免疫反应性。方法 根据毕赤酵母的密码子偏好性优化SCP基因并构建重组质粒。将其热激转化至P. pastoris GS115菌株后经遗传霉素(Geneticin, G418)筛选获得阳性高拷贝子。最后通过甲醇诱导表达重组SCP并结合免疫印记(Western blotting, WB)和间接酶联免疫吸附实验(enzyme-linked immunosorbent assay, ELISA)验证其免疫反应性。结果 SCP在P. pastoris GS115中实现了可溶性高效表达,其表观分子量约为28 kDa。在摇瓶水平下,最佳诱导条件为pH为6.0、每24 h添加1.0%(v/v)甲醇,于28℃发酵144 h,在此条件下,纯度为91.6%的SCP产量可达15 mg/L。WB和间接ELISA结果表明,重组SCP具有IgG结合能力。结论 毕赤酵母表达系统可以得到纯度较高且免疫反应性良好的重组SCP。本研究为SCP的理化研究及产业化应用奠定了基础,并有望促进特异性甲壳类过敏原检测的发展。 相似文献
100.
Strategically differentiated managerial evidence of different social media platforms is of great importance to enhance crisis communication processes by balancing their strengths and weaknesses. This study aims to uncover the platform-specific situational information-sharing characteristics by differentiating the major types of information published in Weibo and WeChat during different phases of a crisis. The subject of the study is the Changsheng fake vaccine crisis which happened in China in 2018. Multiple supervised machine learning and topic modelling methods are used for the characterization of situational information types of the crisis during three phases in both platforms. Our study found that WeChat shares more situational information such as notifications, caution and advice, and criticizing information, whereas Weibo shares more emotional support and help-seeking information. This study provides social media analytics and empirical evidence of platform-specific situational information-sharing characteristics to aid authorities/researchers for better crisis communication and public emergency management. 相似文献