Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape (Brassica napus L.) against Fungal Pathogens

Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of Pseudomonas fluorescens BRZ63, a new endophyte of oilseed rape (Brassica napus L.) against Rhizoctonia solani W70, Colletotrichum dematium K, Sclerotinia sclerotiorum K2291, and Fusarium avenaceum. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of Arabidopsis thaliana Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion.     

无机─矿质复混肥对油菜产量和品质的影响

应用无机－矿质复混肥进行了油菜盆栽试验,结果表明：无机－矿质复混肥可以促进油菜的生长发育,减轻病虫害的发生;施用无机－矿质复混肥比对照增产 ６８．８％～ ７９．１％,达到了极显著水平,比常规肥增产 １４．２％～２１．２％,达到了显著和极显著水平;施用无机－矿质复混肥可以提高油菜的Ｖｃ含量,降低油菜体内硝酸盐含量,改善油菜品质。     

菜籽油基单甘油酯马来酸单酯与苯乙烯共聚条件的探讨

随着石油资源的日益枯竭和环境污染问题的日趋严重,植物油作为一种可再生的绿色生物基资源,以其产量巨大、可储存和碳循环等优点引起全球的广泛关注。本课题采用菜籽油在一定条件下先后与甘油(glycerin)和马来酸酐(MA)反应生成菜籽油基单甘油酯马来酸单酯(ROMG-MA),然后与苯乙烯发生共聚合反应,转化为热固性树脂。讨论分析了各个反应条件对产物总转化率的影响,得出ROMG-MA与苯乙烯共聚合反应的适宜条件为:ROMG-MA质量分数50%、BPO的质量分数5%、苯乙烯的质量分数45%、反应温度90℃,反应时间1.0h。     

菜子饼水解液在L—异亮氨酸发酵中的应用

利用豆饼水解液的方法将菜子饼水解液用于L－异亮氨酸发酵，找到了使用营养缺陷型菌株进行L－异亮氨酸发酵时，菜子饼水解液在种子培养基和发酵培养基的最佳添加量。通过比较，产酸不受影响，在30t吨罐上进行工业化大生产，与实验结果相一致。     

菜子饼水解液在L异亮氨酸发酵中的应用

利用豆饼水解液的方法将菜子饼水解液用于L 异亮氨酸发酵 .找到了使用营养缺陷型菌株进行L 异亮氨酸发酵时 ,菜子饼水解液在种子培养基和发酵培养基的最佳添加量。通过比较 ,产酸不受影响 ,在 30t吨罐上进行工业化大生产 ,与实验结果相一致     

Cognitive processes underlying women's risk judgments: Associations with sexual victimization history and rape myth acceptance.

Objective: This study evaluated the effects of sexual victimization history, rape myth acceptance, implicit attention, and recent learning on the cognitive processes underlying undergraduate women's explicit risk judgments. Method: Participants were 194 undergraduate women between 18 and 24 years of age. The sample was ethnically diverse and composed primarily of freshman, heterosexual, and single women. Stimuli were written vignettes describing social situations that varied on dimensions of sexual victimization risk and potential impact on women's popularity. Participants completed cognitive tasks assessing relative attention to victimization risk versus popularity impact, learning about either risk or popularity impact, and explicit classification of victimization risk. Participants then completed the Sexual Experiences Survey (SES) and the Rape Myth Acceptance Scale; SES responses were used to quantify the severity of victimization experiences. Results: More severe victimization history predicted use of higher thresholds for judging situations as risky, as well as lower sensitivity to risk and greater sensitivity to popularity impact when judging risk. Greater rape myth acceptance also predicted lower sensitivity to risk information. Higher relative attention to victimization risk predicted greater sensitivity to risk information when judging risk. Recent learning about either the risk or the popularity impact aspects of social situations modified sensitivity to risk versus popularity when making risk judgments. Conclusion: The study emphasizes the importance of distinguishing the threshold for judging situations as risky from sensitivity to risk-relevant information in understanding individual differences in women's risk judgments. Both processes may be important to consider when developing interventions to reduce women's risk for sexual victimization. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

油菜主要病害防治研究进展

主要介绍了油菜主要病害的侵染循环、危害症状、识别特征,并概述了油菜病害的农业防治、化学防治、生物防治措施。     

The evaluation of a sexual assault risk reduction program: A multisite investigation.

This article summarizes the results of the Ohio University Sexual Assault Risk Reduction Project, which is a program designed to reduce college women's risk for sexual assault. The program was evaluated at 2 separate universities with 762 women. Participants were randomly assigned either to the program or to the no-treatment comparison group, and they completed measures that assessed sexual victimization, dating behaviors, sexual communication, and rape empathy at the pretest and at the 2-month and 6-month follow-ups. At the 2-month follow-up, there were no differences between the groups on any of the outcome measures. However, those women who were moderately victimized during the 2-month follow-up were significantly less likely to be revictimized during the 6-month follow-up period if they participated in the program. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

菜籽粕热压法脱毒新工艺研究

本文阐述了热压法去毒的原理，分析了降解时间和多元降解助剂对去毒的影响，为工业化大规模生产脱毒菜籽粕提供了依据     

菜籽油脂肪酸二乙醇酰胺一步合成法

本文讨论了以菜籽色拉油与二乙醇胺直接反应一步合成脂肪酸二乙醇酰胺。经过条件优化法确定了较好的合成工艺，具体条件为：(I)物质的量投料比(菜籽色拉油：二乙醇胺)为1：3．7～4．O，反应温度120～125℃，反应时间2．5～3．0h，ω(催化剂）=1．5％，菜籽色拉油中脂肪酸的转化率90％左右。副产物甘油可以用混合溶剂分离出来，在得到纯度高的脂肪酸烷醇酰胺的同时，还得到副产物甘油．