Proteomic analysis of the kidney filtration barrier—Problems and perspectives

Diseases of the glomerular filter of the kidney are a leading cause of end-stage renal failure. The kidney filter is localized within the renal glomeruli, small microvascular units that are responsible for ultrafiltration of about 180 liters of primary urine every day. The renal filter consists of three layers, fenestrated endothelial cells, glomerular basement membrane, and the podocytes, terminally differentiated, arborized epithelial cells. This review demonstrates the use of proteomics to generate insights into the regulation of the renal filtration barrier at a molecular level. The advantages and disadvantages of different glomerular purification methods are examined, and the technical limitations that have been significantly improved by in silico or biochemical approaches are presented. We also comment on phosphoproteomic studies that have generated considerable molecular-level understanding of the physiological regulation of the kidney filter. Lastly, we conclude with an analysis of urinary exosomes as a potential filter-derived resource for the noninvasive discovery of glomerular disease mechanisms.     

大型输水工程阀门监造招标文件的编制

本文针对某工程阀门监造招标文件的编制进行了详尽的分析。该招标文件是在水利工程监理招标文件范本的基础上,参考其他行业监造合同范本,并针对其工程特点修改而成。文中阐述了招标文件中商务部分、技术文件、监造大纲、评标标准及方法的编制,以期为其他类似工程项目招标文件编制提供借鉴。     

基于ASP的植物生理图片系统的实现

为了满足我院《植物生理》理论教学和实践教学中图片展示的需要,弥补理论教学的不足,并为远程网络教学提供网络资源;从植物生理图片的管理出发,分析了《植物生理》教学的要求,以教学满足教学要求为目的,采用B/S架构,针对植物切片扫描成的图片进行数字化管理的要求,开发出一套适合切片图片管理的系统.系统实现了切片图片的存储、管理、...     

TiO2纳米管的制备方法及其改性

本文对TiO2纳米管制备技术、特点等进行了综述，且探讨了TiO2纳米管的改性方法及存在问题，并对今后的发展方向予以展望。     

新型耐腐蚀不饱和聚酯树脂的制备

根据高分子化学和材料腐蚀理论，设计耐腐蚀不饱和树脂的化学结构，并依据其特对树脂的化学结构进行优化，确定了新型耐腐蚀不饱和树脂的生产配方和工艺，经测试其耐腐蚀性能优良。     

Preparation method for supported metal catalysts using w/o microemulsion: Study on immobilization conditions of metal particles by hydrolysis of alkoxide

It was previously found that the silica-supported rhodium catalyst prepared using water-in-oil microemulsion had rhodium particles partly, or wholly, embedded in silica. In this work, consequently, we investigated the effect of hydrolysis conditions of tetraethylorthosilicate, employed as the source of silica, on the atomic ratio of surface rhodium in contact with the gas phase, to total surface rhodium of nanoparticles. This ratio is denoted as R in this paper. R became higher when the catalyst was prepared under the following hydrolysis conditions: a shorter hydrolysis time and a smaller amount of tetraethylorthosilicate. On the other hand, R showed the minimum value when the water content in the preparation solution was 33 vol%. From these results, it is demonstrated that it was important to form silica as early as possible in hydrolysis of TEOS in order to increase R values. In addition, the effect of R on the catalytic behavior in CO hydrogenation was investigated. At R values below 30%, the turnover frequencies increased with a decrease in R.     

Preferential grain growth and improved fatigue endurance in Sr substituted PZT thin films on Pt(1 1 1)/TiOx/SiO2/Si substrates

Pb(Zr_0.5Ti_0.5)O₃ and (Pb_0.9Sr_0.1)(Zr_0.5Ti_0.5)O₃ thin films were grown on Pt/TiO_x/SiO₂/Si substrates by chemical solution deposition. 10% Sr substituted PZT film showed high degree of (0 0 1) type preferential grain growth. Surface morphology revealed a clear correlation between preferred grain orientation and grain size. Room temperature dielectric constant was 1200 and 700 for the PZT and PSZT films, respectively. Dielectric loss reduced with Sr substitution. PZT film showed severe fatigue, and hence the polarization reduced to 20% of the initial value (24 μC/cm²) after 10⁸ cycles where as PSZT showed less fatigue, 75% of the initial polarization (12 μC/cm²) was retained after 10⁸ switching cycles.     

Fabrication of novel sol-gel silica coatings reinforced with multi-walled carbon nanotubes

Novel sol-gel silica coatings reinforced with multi-walled carbon nanotubes (MWCNTs) have been prepared using the organic sol-gel route and the dip-coating technique on magnesium alloy substrates. Homogeneous and dense composite coatings with good reinforcement dispersion were fabricated using low temperature and atmospheric pressure fabrication conditions. The presence of nanotubes caused a substantial enhancement of silica coating fracture toughness on coatings deposited on grounded substrates but it was not as effective on polished substrates because of the low adhesion of the coating to the substrate. Bridging phenomena caused by the MWCNTs was observed, indicating that an effective load transfer between the silica matrix and the nanotube reinforcement was also achieved.     

Fine-sized BaMgAl10O17:Eu phosphor powders prepared by spray pyrolysis from the spray solution with BaF2 flux

Fine-sized BaMgAl₁₀O₁₇:Eu²⁺ phosphor powders with plate-like morphology were prepared by spray pyrolysis process. The effects of ratio of BaF₂ and Ba(NO₃)₂ used as the source materials of Ba component on the morphological and optical properties of the BaMgAl₁₀O₁₇:Eu²⁺ phosphor powders were investigated. BaF₂ was used as the flux material as well as the source material of Ba component. The phosphor powders prepared from the spray solution with the same mole concentrations of BaF₂ and Ba(NO₃)₂ had fine size, plate-like morphology and narrow size distribution. The addition of BaF₂ as the source material of Ba component increased the photoluminescence intensities of the phosphor powders. The phosphor powders prepared from the spray solution with the ratios of BaF₂ and Ba(NO₃)₂ larger than 1 had the similar photoluminescence intensities to that of the commercial product.     

Basic Methods for Preparation of Liposomes and Studying Their Interactions with Different Compounds,with the Emphasis on Polyphenols

Studying the interactions between lipid membranes and various bioactive molecules (e.g., polyphenols) is important for determining the effects they can have on the functionality of lipid bilayers. This knowledge allows us to use the chosen compounds as potential inhibitors of bacterial and cancer cells, for elimination of viruses, or simply for keeping our healthy cells in good condition. As studying those effect can be exceedingly difficult on living cells, model lipid membranes, such as liposomes, can be used instead. Liposomal bilayer systems represent the most basic platform for studying those interactions, as they are simple, quite easy to prepare and relatively stable. They are especially useful for investigating the effects of bioactive compounds on the structure and kinetics of simple lipid membranes. In this review, we have described the most basic methods available for preparation of liposomes, as well as the essential techniques for studying the effects of bioactive compounds on those liposomes. Additionally, we have provided details for an easy laboratory implementation of some of the described methods, which should prove useful especially to those relatively new on this research field.