响应面法优化慈姑甘蔗汁复合澄清剂

以透光率为指标,利用响应面试验优化慈姑甘蔗汁的复合澄清剂配方,实验得出慈姑甘蔗汁澄清的最佳工艺条件为:瓜尔豆胶用量0.06％,明胶用量0.20％,羧甲基纤维素钠用量0.25％.用优选方案所制备的慈姑甘蔗复合饮料的透光率为89.438％,澄清效果好.     

Expression Patterns of Sugar Transporter Genes in the Allocation of Assimilates and Abiotic Stress in Lily

During the growth cycle of lilies, assimilates undergo a process of accumulation, consumption and reaccumulation in bulbs and are transported and allocated between aboveground and underground organs and tissues. The sink–source relationship changes with the allocation of assimilates, affecting the vegetative growth and morphological establishment of lilies. In this study, the carbohydrate contents in different tissues of five critical stages during lily development were measured to observe the assimilates allocation. The results showed bulbs acted as the main source to provide energy before the budding stage (S3); after the flowering stage (S4), bulbs began to accumulate assimilates as a sink organ again. During the period when the plant height was 30cm with leaf-spread (S2), leaves mainly accumulated assimilates from bulbs through the symplastic pathway, while when leaves were fully expanded, it transformed to export carbohydrates. At the S4 stage, flowers became a new active sink with assimilates influx. To further understand the allocation of assimilates, 16 genes related to sugar transport and metabolism (ST genes) were identified and categorized into different subfamilies based on the phylogenetic analysis, and their protein physicochemical properties were also predicted. Tissue-specific analysis showed that most of the genes were highly expressed in stems and petals, and it was mainly the MST (monosaccharide transporter) genes that were obviously expressed in petals during the S4 stage, suggesting that they may be associated with the accumulation of carbohydrates in flowers and thus affect flower development process. LoSWEET14 (the Sugar will eventually be exported transporters) was significantly correlated with starch in scales and with soluble sugar in leaves. Sugar transporters LoHXT6 and LoSUT1 were significantly correlated with soluble sugar and sucrose in leaves, suggesting that these genes may play key roles in the accumulation and transportation of assimilates in lilies. In addition, we analyzed the expression patterns of ST genes under different abiotic stresses, and the results showed that all genes were significantly upregulated. This study lays a solid foundation for further research on molecular mechanism of sink–source change and response to abiotic stresses in lilies.     

过氧化物漂白对蔗渣木聚糖提取的影响

比较了直接浸提和预处理后再浸提对蔗渣木聚糖提取率和产物的影响。蔗渣经过碱性过氧化氢或过氧乙酸的漂白预处理,有利于促进木聚糖的溶出和提高提取率,尤其是碱性过氧化氢的促进作用更显著,可以使木聚糖提取率达到21.5%(对干料),精制后产物纯度和白度分别达到85%和83%,但对于木聚糖纯度的提高并不是很显著。不同提取方式所得到的木聚糖在糖基组成和红外光谱图均有很高的相似度。     

甜菜与BNYVV互作过程中PR基因的诱导表达

以抗病性不同的4个甜菜品种为材料,分别接种BNYVV,研究甜菜与BNYVV互作过程中几丁质酶、β-1,3-葡聚糖酶活性和诱导几丁质酶、β—1,3-葡聚糖酶基因表达及其与抗丛根病的关系。结果表明：BNYVV不同程度地诱导了4个甜菜品种几丁质酶、β—1,3-葡聚糖酶活性的提高,抗病品种比感病品种具有较高的几丁质酶和β—1,3-葡聚糖酶活性,并且抗病品种的酶活性高峰出现的时间早于感病品种;抗病品种的几丁质酶、β-1,3-葡聚糖酶基因的表达均早于感病品种,表达量大于感病品种,且持续的时间较感病品种长。BNYVV不同程度地诱导甜菜病程相关蛋白基因的表达,从而有效激活了甜菜抗病防御反应系统,提高了甜菜抗病性。     

离子色谱法测定白砂糖中氟的含量

本文建立离子色谱法测定白砂糖中氟含量的方法。采用IonPac AS18阴离子色谱柱分离,氢氧化钾溶液等度淋洗,抑制电导检测。仪器检出限为0.02 mg/L,加标回收率为99.21%～101.1%,相对标准偏差0.82%～1.23%（n=6）。结果表明,方法简便快速准确。     

食品中还原糖含量测量的不确定度评估

根据JJF1059-999《测定不确定度的评定与表示》和GB／T5009．7——2003食品中还原糖含量的测定原理，建立不确定度的数学模型。系统分析并计算食品中还原糖测量全过程的不确定度各分量、结果标准不确定度及扩展不确定度．此方法适用于评估食品中还原糖含量的不确定度。     

归德白糖蒜的生产技术

商丘古称归德，归德白糖蒜是河南商丘传统的土特产品之一，风味独特，深受消费者喜爱。该文介绍了归德白糖蒜的原辅料配比、工艺流程、操作要点、成品质量要求及注意事项等。     

甜菜含糖率与根重关系的研讨

本文对24个甜菜品系的根重与含糖率相关性进行了研究分析。结果表明：其中22个品系的单株根重与单株含糖率表现为无相关性，2个品系的单株根重与单株含糖率表现为显著负相关；为了进一步研究，我们对24个品系的平均单株含糖率与平均单株根重进行了相关分析；结果为二者无相关。如果这一结论具有广泛性，将对甜菜育种研究起到重要作用。     

甘蔗糖液中C1~-和SO_4~(2-)的紫外线检测──离子色谱法测定

提出了采用紫外检测——高效离子色谱法测定甘蔗糖液样品中的Cl-和5O42-的分析方法。色谱分离系统由yydac302IC离子色谱柱和3mmol／L邻苯二甲酸溶液（PH＝5．0，用四硼酸钠调节）组成。利用流动相具有的紫外光吸收特性，周紫外光度检测器对分离后的Cl-和SO42-进行间接检测。     

基于siPLS的猕猴桃糖度近红外光谱检测

为了探寻一种快速无损检测猕猴桃糖度的方法,利用小波滤噪法对猕猴桃1000～2500nm 近红外光谱进行了预处理,并用偏最小二乘法(PLS)、区间偏最小二乘法(iPLS)和联合区间偏最小二乘法(siPLS)分别建立预测模型。结果表明,采用联合区间偏最小二乘法将光谱划分为16 个子区间,利用其中的第9、11、13 号3 个子区间联合建立的糖度模型效果最佳,其校正集相关系数和均方根误差分别为0.9414 和0.3788。预测集相关系数和均方根误差分别为0.9295 和0.3904,主因子数为7 个。研究表明,用小波滤噪和联合区间偏最小二乘法所建立的猕猴桃糖度模型不但减少建模运算时间,剔除噪声过大的谱区,而且预测能力和精度均有所提高。