A protein which masks galactose receptor mediated phage susceptibility in Lactococcus lactis subsp. lactis MPL56

A 28.5-kb plasmid, isolated from Lactococcus lactis subsp. lactis MPL56, causes complete inhibition of four lactococcal phages. Cell wall characteristics of wild-type strain MPL56 were compared with its 28.5 kb plasmid-cured, phage-sensitive derivative MPL56-22. After proteolytic enzyme treatments, adsorption of phages occurred at high levels, an example is 94.6–98.5% in MPL56 cells. Analysis of cell wall extracts of MPL56-22 by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) indicated that the only difference between strains was the 55.4 kDa band in protein patterns of MPL56. Adsorption of the four phages was completely inhibited when MPL56-22 cells were subjected to SDS, Triton-X-100, HCl and NaOH treatments. Lectins that were specific for glucose/mannose and N-acetylglucosamine did not prevent adsorption of phages in cell wall extracts of MPL 56-22. However a lectin specific for galactose (MCA; Momordica charantia ) completely inhibited adsorption of these phages in cell wall extracts of MPL56-22. HPLC patterns of cell wall carbohydrates of MPL56-22 and its HCl treated preparations showed that the most prevalent difference was the galactose on untreated MPL56-22 cell wall chromatograms.     

用瑞利波检验高喷防渗墙的施工质量及地层分布特征

本文阐述了瞬态多道瑞利波探测的原理和方法，并将其应用到大堤高喷防渗墙施工质量的检验中，取得一定成效，测试结果与测压管观测结果对比，相互吻合，为类似工程的质量检验提供了一种可采用的检测手段。     

Aggregate States and Energetic Disorder in Highly Ordered Nanostructures of para‐Sexiphenyl Grown by Hot Wall Epitaxy

We report on photoluminescence (PL) and thermally stimulated luminescence (TSL) in highly ordered nanostructures of para‐sexiphenyl (PSP) grown by hot wall epitaxy (HWE). A low‐energy broad band is observed in the PL spectra that can be attributed to the emission from molecular aggregates. While the intrinsic exciton emission in steady‐state PL dominates at low temperatures, the emission from aggregates increases with elevating temperature and its magnitude depends sensitively on film preparation conditions. Time‐resolved PL measurements showed that the aggregate emission decays with a life‐time of ≈ 4 ns, which is approximately an order of magnitude larger than the lifetime of singlet excitons. TSL data suggests the presence of an energetically disordered distribution of localized states for charge carriers in PSP films, which results from an intrinsic disorder in this material. A low‐temperature TSL peak with the maximum at around 30 K evidences for a weak energy disorder in PSP films, and has been interpreted in terms of a hopping model of TSL in disordered organic materials.     

THE STICKY ISSUES OF DRYING

Unconrolled agglomeration of particles and adhesion of the particulate mass to wall surfaces are common severe problems in many drying operations. This paper reviews the mechanisms of interparticle and particle-surface attraction as well as the theory and the existing tests for characterising powders in terms of internal strength, wall friction and adhesion. The usefulness of the information obtained from these tests in dryer design and operation is critically reviewed. The importance of powder stickiness in various types of dryers is discussed and established methods of combating the problem are presented Recommendations an made for further research in the area of powder cohesion and adhesion.     

台阶爆破岩石破碎块度分布的数学模型

以ＫＵＺ－ＲＡＭ模型为基础，提出了岩石系数和均匀性指数的确定方法，由此建立的数学模型在水厂铁矿爆破试验中得到了验证。     

构皮滩水电站尾水隧洞特大断面软岩开挖支护

构皮滩水电站尾水隧洞软岩不良地质洞段占尾水隧洞全长的70%以上,确保软岩洞段的安全快速开挖是尾水隧洞施工的关键,尤其一次支护的强度对洞室稳定影响极大。为此,施工中根据软岩的地质特点及尾水隧洞开挖的断面尺寸,采取分层开挖和超前锚杆的施工方式和型钢拱架进行一次支护。根据监测结果,围岩变形很小,一次支护效果良好,表明构皮滩水电站尾水隧洞大断面软岩采取的开挖方式和一次支护方式是成功的。     

大伙房水库输水工程隧洞结构应力仿真分析

应用有限元法对辽宁省大伙房水库输水工程主体隧洞结构进行了仿真分析,从而得到隧洞结构的应力分布规律,研究确定薄弱部位并采取相应的稳定措施,以便保证隧洞施工的安全与经济。     

缝洞型碳酸盐岩油藏流动单元概念和研究方法探讨

流动单元对于提高油田采收率具有很大的实际意义,该项研究在砂岩油藏研究中已取得较大进展。对于缝洞型碳酸盐岩油藏,由于岩石结构构造、成岩后生作用、构造断裂作用、溶蚀作用等多因素对储集空间的影响,已有的流动单元概念及研究技术方法难于应用。以塔河油田为例,针对缝洞型碳酸盐岩地质特征,论证了碳酸盐岩储层渗流屏障的存在及其类型,探索性提出区别于砂岩油藏的缝洞型碳酸盐岩储层流动单元的概念。提出的研究思路和方法包括;油藏压力趋势分析法、井间生产干扰分析法、流体性质差异分析法、井间干扰试井法。结合储层发育特征,初步建立了缝洞型碳酸盐岩流动单元的划分标准。     

QP19-L型PDC钻头在TK320井的应用

针对塔河地区的地层特点和PDC钻头的破岩机理，优选了适合于该地层钻进的QP19-L型PDC钻头，总结了PDC钻头的使用技术措施。在TK320井二开井段的应用表明，该钻头能明显提高机械钻速，缩短钻井周期，值得推广应用。     

可控环量船用翼的数值模拟和试验研究

本文提出一种可控环量船用翼数值模拟方法,其计算结果与最近Mclachlan的试验数据十分吻合。通过本文的计算,对广泛引用的Kind ＆ Maull的二维试验数据提出了修正。根据本文所作的三维可控环量帆翼的试验结果,与二维计算值的比较,指出它们之间所存在的显著差别。