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61.
Interfacial hybridization kinetics of oligonucleotides immobilized onto fused silica surfaces 总被引:3,自引:0,他引:3
Jiang Zeng Amer Almadidy James Watterson Ulrich J. Krull 《Sensors and actuators. B, Chemical》2003,90(1-3):68-75
Fused silica optical fibers have been used in an intrinsic mode optical configuration as biosensors for fluorescence based detection of hybridization of nucleic acids. In this work, the kinetics of hybridization of single-stranded oligonucleotides that were covalently immobilized were studied. The probe DNA was dT20, and the target was Fluorescein-labeled non-complementary (dT20) or complementary (dA20) oligonucleotide. Chronofluorimetric monitoring of the adsorption and hybridization processes was used to investigate oligonucleotide films of different density, in different salt concentrations, at temperatures of 25 and 40 °C, with the concentration of the target DNA being 0.005–0.1 μM. Mathematical models based on first- and second-order Langmuir adsorption have been examined to describe both the adsorption and the hybridization processes. Experimental data were processed using the models, and the hybridization kinetics were calculated. Hybridization kinetics on these optical fiber DNA sensors was found to be up to three orders faster than results presented for a number of other experiments using different immobilization chemistries. 相似文献
62.
XIAKe-yu YURong-jin MENGHua-mao 《半导体光子学与技术》2004,10(2):82-85
By stretching the rod waveguide with different velocities in opposite directions, the tapered waveguide can be fabricated. In condition of taking no account of volume expansion caused by heating and under the assumptions of volume conservation,the rod waveguide can be stretched freely in the heated region without being stretched outside of the heated region. A model,which shows the relation of the transition shape and the two factors, that is the ratio of two velocity and the heated region length, is presented for the shape of the taper transition through mathematic deduction. Based on this model, a desired tapered waveguide can be fabricated. The tapered waveguide are widely used for fabricating tapered fiber couplers and sensors. In addition, the conclusion can be used for fabricating fused fiber coupler. 相似文献
63.
CHENSheng-ping LUKe-cheng LIYi-gang LIJia-fang ZHUJian-ping FENGMing LIJing 《半导体光子学与技术》2004,10(2):122-126
The effects of the variations in fiber length, fiber mirror reflectance on efficiency and output power are experimentally investigated for erbium-doped double pass backward superfluorescent fiber sources (SFSs). The influence of fiber length on mean wavelength stability (MWS) has also been demonstrated. By incorporating a short section of un-pumped erbium-doped fiber (EDF) at the output port, the pump power dependent on MWS becomes independent of pumped EDF length. This is a novel phenomenon that hasn‘t been reported up to now,and should be helpful to SFS fabrication and theory analysis. By using a fiber Michelson interferometer as spectrum slicing component,a multi-wavelength fiber source (MWFS) with ~20 channels (from 1542nm to 1559nm) is got. The MWFS has a channel spacing of ~0. 8 nm which satisfies ITUstandard. The intensity fluctuation among channels is less than 0.5dB,and the extinction ratio of all channels is above 14dB. This kind of MWFS should be useful to wavelength division multiplexing systems. 相似文献
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聚乙烯丙纶复合防水卷材的特点及其应用 总被引:2,自引:0,他引:2
介绍了聚乙烯丙纶复合防水卷材的产品结构特点和性能特点、防水系统结构特点和配套材料、应用拼接技术以及产品应用范围。 相似文献
66.
Stretching dominated Kagome lattices reinforced by carbon fibers were designed and manufactured. The sandwich panels were assembled with bonded laminate skins. The mechanical behaviors of the sandwich panels were tested by out-of-plane compression, in-plane compression and three-point bending. Different failure modes of the sandwich structures were revealed. The experimental results showed that the carbon fiber reinforced lattice grids are much stiffer and stronger than foams and honeycombs. It was found that buckling and debonding dominate the mechanical behavior of the sandwich structures, and that more complaint skin sheets might further improve the overall mechanical performance of the sandwich panels. 相似文献
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用单透镜变换由多模光纤出射的大功率Nd:YAG激光束的特性 总被引:1,自引:0,他引:1
根据理论假设,通过选取单透镜的参数和位置,计算说明了单透镜使经由多模光纤出射的大功率Nd:YAG激光束的发射角压缩,光束聚焦效率提高,光束截面场为高斯分布;文中的计算结果符合已知规律,从而说明了理论假设的正确性。 相似文献
70.
利用挤压铸造制备氧化铝/锌合金复合材料,在扫描电镜(SEM)上观察复合材料的界面。结果表明,在复合材料中纤维与基体间存在致密界面层,合金元素通过适当的化学反应可改善纤维与基体间的结合;在凝固过程中,纤维/基体界面上的硅在共晶体的共生生长过程中起了领先相作用,导致复合材料的共晶转变是由铝硅共晶转变和锌铝共晶转变两者组成。 相似文献