低温等离子接枝改性聚乳酸支架的亲水性

聚乳酸具有良好的生物相容性以及可降解性能,但其强疏水性能一定程度上限制了其在可降解支架领域的应用。本研究利用氩气(Ar)低温等离子技术在PDLLA血管支架表面接枝亲水性的聚合物聚乙烯吡咯烷酮(PVP)以及聚醋酸乙烯酯(PVAc)。接触角测试表明,经等离子接枝的材料表面接触角由原来的105°~110°可降至22°,由强疏水性变为亲水性。体外细胞粘附性实验表明:等离子处理后的材料表面表现出亲水性质,其细胞的粘附性也得以明显提高,更加有利于细胞在其表面的生长。     

细胞自动机：简单的规则，复杂的行为

细胞自动机是一种时间、空间与状态都离散的数学模型。回顾了初等细胞自动机模型的应用及其符号动力学刻画的相关文献,在大量统计性质和计算机模拟基础上,着重分析具有鲁棒Bernoulli移位特征的细胞自动机规则的一些符号动力学性质,揭示了细胞自动机的简单规则中蕴含着复杂、混沌的非线性动力学特性。这些结果丰富了细胞自动机的理论基础,也将促进符号动力系统的理论和应用的研究。     

A Novel Fluorescence Probe 9-（4-（1,2-diamine）benzeneN 1-phenyl）acridine for Nitric Oxide Determination

A novel fluorescent probe 9-(4-(1,2-diamine)benzene-N1-phenyl)acridine(DABPA) was synthesized for the detection of nitric oxide(NO) and characterized by IR, 1H-NMR and EI-MS spectroscopy. Based on a photoelectron transfer mechanism, the fl uorescence intensities of DABPA were investigated with the different concentrations of NO. Under the optimal experimental conditions, the fl uorescence intensity of DABPA had a good linear relationship(R2=0.9977) with NO concentration in the range from 1×10-7 to 1.5×10-6 mol/L with a detection limit of 1×10-8 mol/L. The cytotoxicity induced by DABPA was evaluated by the MTT(3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide) assay for biological application. Furthermore, the probe DABPA had also been successfully applied to real-time image NO produced in PC12 cells in the presence of L-arginine.     

Preparation and characterization of PEGDE crosslinked silk fibroin film

To obtain water-insoluble silk fibroin(SF) materials, polyethylene glycol diglycidyl ether(PEG-DE) was selected as a crosslinking agent to prepare SF films(blends). The reaction conditions were optimized for the crosslinking of the SF molecules. The hot water stability of the blends was measured using BCA protein assay and gravimetric analysis. The molecular conformation and crystalline structure of the blends were analyzed by FTIR and XRD, respectively. When the mass ratio of SF:PEG-DE was 1.0:0.8, the hot water loss rate of the SF blends was minimized. PEG-DE could induce SF molecules to form β-sheets during the gel reaction process, resulting in improved crystallinity and hot water dissolved resistance of the blend films. In order to demonstrate the cytotoxicity of the chemical reagents used to crosslink SF, L929 cells were seeded on the blend film(SF:PEG-DE = 1:1) and cultured for 3 days. Cells of L929 readily adhered and spread in the fusiform on the blend film resulting in high cell viability. The extracted liquid from the SF porous film did not inhibit cell proliferation, as estimated by the MTT assay.     

Nutlins类似物诱导TCA-8113细胞凋亡的研究

目的:研究新型小分子化合物Nutlins类似物在体外对舌癌TCA-8113细胞的凋亡作用.方法:采用MTT法检测新型小分子化合物Nutlins类似物对TCA-8113细胞增殖的影响;用流式细胞仪(FCM)检测Nutlins类似物诱导TCA-8113细胞凋亡情况和周期阻滞情况;用DAPI染色法观察细胞核变化.结果:Nutlins类似物NL-11和NL19以浓度依赖的方式抑制TCA-8113细胞增殖并诱导其凋亡,而且能引起TCA-8113细胞发生G2期周期阻滞.结论:NL-11和NL-19化合物能够有效地在体外抑制TCA-8113细胞增殖,并诱导细胞凋亡.     

突变型P53特异性活化T细胞的诱导与扩增

采用多种细胞因子诱导外周血单核细胞分化为成熟的树突状细胞（DC,Dentritic Cell）,将成熟DC与初始T细胞共培养,并连续加入DC刺激从而避免活化的T细胞凋亡。将活化的T细胞转入包被有CD3和CD28抗体的板中,使活化T细胞进一步大量扩增。流式检测多因子诱导后DC表型发现,高表达CD83、CD11c、CD11b、CD80、CD86、CCR7、CD54,低表达CD14、CD3、CD33,说明因子成功诱导单核细胞为成熟DC。流式检测扩增的活化T细胞发现高表达CD3、CD8,含有很低量的调节性T细胞（Treg）,分泌大量的IFN-γ,表明扩增出的细胞中活化T的含量很高,为临床研究提供参考。     

简讯

英国技术创新中心发展顺利 英国已建设了先进制造、卫星应用、细胞疗法、近海可再生能源、未来城市、交通系统和联通数字经济等7个关键领域技术创新中心。在此基础上,英国计划于2015～2016年成立能源系统领域和分层医学诊断领域技术创新中心,进一步推动相关行业科技成果转化。     

胡椒碱对人胃癌SGC-7901细胞抗肿瘤活性的体外实验研究

胡椒碱（Piperine）是一种从胡椒属植物中提取的生物碱,具有镇静、抗炎、抗肿瘤等多种药理活性.探讨胡椒碱对人胃癌SGC-7901细胞的增殖抑制和诱导凋亡的作用.采用MTT比色法测定其对SGC-7901细胞增殖抑制率;通过免疫荧光法、流式细胞术、Western blot法对其进行抗癌机制研究.MTT结果显示Piperine处理细胞72h的IC50值为37.41 μmol·L-1,且呈现时间剂量依赖性; Hoechst33258染色荧光显微镜观察发现Piperine能诱导SGC-7901细胞核形态学改变,部分细胞呈现典型的凋亡形态学特征;Annexin V-FITC/PI荧光双染结果亦证实Piperine可以诱导SGC-7901细胞发生凋亡;DAPI和DCFH-DA染色流式细胞术分析显示Piperine诱导SGC-7901细胞G2/M期阻滞、细胞活性氧产生增加; Western blot分析发现Bcl-2蛋白表达减少,Bax蛋白表达逐渐增加,呈现剂量依赖性,且Bax/Bcl-2比例增加.因此,Piperine具有抑制SGC-7901细胞增殖和诱导凋亡的抗肿瘤活性.     

Al2O3/Cu复合材料强化机理研究

采用内氧化法制备了Al2O3／Cu复合材料，进行了高温电子拉伸实验，并通过微观组织观察，分析了该复合材料的强化机理。拉伸实验结果显示：Al2O3／Cu复合材料不仅室温强度很高，而且高温时仍保持较高的强度。微观组织观察分析表明：细小的Al2O3颗粒的弥散分布是该复合材料具有高强度的主要原因，表现在：Al2O3颗粒存在能够抑制Cu基再结晶的进行；Al2O3颗粒的存在阻碍晶界亚晶界运动，从而阻碍晶粒长大；Al2O3颗粒的阻碍位错运动，增加位错密度；Al2O3颗粒的存在提高材料的蠕变抗力。     

Creep behavior and microstructure evolution of directionally solidified superalloy IC 10 at high temperature

The creep behavior of directionally solidified （DS） superalloy IC 10 was investigated under 192 MPa and 218 MPa at 980 ℃. Under the testing conditions, the marked creep characteristic of the superalloy is that the creep curve has a short primary and secondary stage and a long tertiary stage. Another creep characteristic is that the superalloy has excellent plasticity at high temperature. To study the creep behavior, the microstructure was observed by SEM and TEM. Different from other microstructure of Ni-base superalloys, superalloy IC10 forms incompletely rafted γ＇ phase during the creep processes. To understand the creep deformation mechanism of superalloy IC10, the movement of dislocations was analyzed. The results show that the dislocations moving in the γ matrix and climbing over the γ＇ precipitates is the main deformation mechanism under the experimental conditions.