氨基酸液生产过程中污染茵的鉴定

从氨基酸液中分离出8株菌,命名为ZH．1—zH一8。7株属十细菌,1株属于霉菌。利用16SrDNA区序列分析并结合彤念学、生邪学特征对细菌菌株进行鉴定。其中,ZH-4为Psychrobacter,ZH-5为Lysinibacilus,ZH-6为Acrococcusviridians,ZH-7为Bacilluspumilus;ZH．3与Planococcus citrcus,ZH-2与Kocuriapalustris,ZH-1与Bacilluspumflus可能为近缘种,有待进一步鉴定。牛弹牛化鉴定结粜与模式菌株相吻合的菌株为ZH-2～ZH-7。18SrDNA区序列分析结合形态学特征鉴定ZH一8为Pcnicillium。ZH-1-ZH-8存GcnBank的登录号依次为JQ819720-JQ819726,JX192597。     

保温发酵鱼露中细菌群落的16S rDNA分子生态分析

本文通过对细菌16S rDNA V₄区进行高通量测序,并结合实时荧光定量PCR 法(FQ-PCR)研究35 ℃发酵条件下鱼露的细菌群落多样性。研究发现发酵初期细菌数量快速下降,到后期数量基本保持稳定。鱼露中细菌群落组成复杂,在测序得到的所有细菌OTUs中共注释出37个门、238个属,但有50%左右的OTUs未能注释到属。发酵8个月后,继续增加发酵时间,鱼露中的细菌组成变化不明显。在门的水平上,在鱼露发酵的各个阶段中细菌主要分布在变形菌门(Proteobacteria)和厚壁菌门(Firmicutes);在属分类的水平上,优势菌主要有乳杆菌属(Lactobacillus)、嗜冷杆菌属(Psychrobacter)、假单胞菌属(Pseudomonas)、链球菌属(Streptococcus)、不动菌属(Acinetobacter)等。发酵前2个月,优势菌是嗜冷杆菌属(Psychrobacter,23.39%)、链球菌属(Streptococcus,5.34%)、乳杆菌属(Lactobacillus,3.05%),发酵12个月时,主要优势菌中变形菌门中以链球菌属(Streptococcus,3.62%)、乳杆菌属(Lactobacillus,3.23%)、葡萄球菌属(Staphylococcus,1.20%)为主,而厚壁菌门以不动菌属(Acinetobacter,9.14%)、嗜冷杆菌属(Psychrobacter,8.77%)、假单胞菌属(Pseudomonas,6.78%)为主,其中耐盐和嗜盐的乳酸菌主要有乳杆菌属(Lactobacillus)、链球菌属(Streptococcus)等。     

黄酒对便秘模型小鼠通便及肠道菌群的影响

研究黄酒对小鼠通便功能作用及便秘小鼠肠道菌群的影响。 体质量、小肠墨汁推进率、首粒黑便排出时间、粪便质量实验结 果表明,高剂量黄酒能够延缓小鼠体质量增长,黄酒未能显著促进便秘小鼠肠道蠕动、缩短首粒黑便排出时间,未能显著增加便秘小 鼠粪便质量。16S rDNA测序分析结果显示,高剂量和中剂量组在物种丰富度上显著高于模型对照组（P＜0.05）;高剂量黄酒能够上调 Akkermansia muciniphila、乳杆菌的比例;下调拟杆菌（Bacteroides）、肠球菌（Enterococcus）、梭菌（Clostridium）和变形杆菌（Proteus）的 比例。 在实验剂量下,未见黄酒具有明显的通便功能作用,黄酒对便秘小鼠肠道菌群结构有调节作用。     

16S-23S rRNA ITS-AFLP指纹图谱分析在白酒窖泥原核微生物多样性分析中的应用

利用非培养的分子生物学方法对位于多粮原料生产的窖壁和窖底的新窖和老窖窖泥中原核微生物的16S-23S rRNA ITS进行AFLP（amplified fragment length polymorphism）分析,利用NTSYSpc2．10e软件构建聚类图,分析其关系。结果表明,来自不同窖池的不同部位的原核微生物在16S-23SrRNAITS水平上呈现出差异,其中老窖窖底和新窖窖底的原核微生物多样性存在着明显差异,其相关系数为0．47;而老窖窖壁和新窖窖壁的原核微生物多样性却差异不大,其相关系数为1．00。     

一株产L-乳酸菌株的筛选、鉴定及营养条件的初步研究

作者对筛选的一株产L-乳酸的菌株进行了菌种鉴定,根据16S rDNA序列测定结果,结合其形态特征及生理生化性质,确定该菌株为凝结芽孢杆菌(Bacillus coagulans)。进一步对其营养条件进行了研究,初步确定玉米糖化液为最适碳源,采用1g/dL酵母粉与1g/dL棉籽蛋白的混合氮源,L-乳酸产量不仅有所提高且成本大大降低,金属离子Mn2+、Fe2+、Mg2+都可在一定程度上提高L-乳酸的产量。     

0癌症进展Oncology Progress5R-5E0546E

ieved in the study of gynecological cancers,early diagnosis,early treatment and valid recognition of precancerous stage are still the priority of therapy.The standardization of treatment on gynecological cancers remains to be generalized and the surgeries are prone to be humanize and individuate.The technique of clinical treatment should be based on evidence-based medicine and cooperation of multi-ceneri和L.hilgardii无法加以区分,但是仍有助于将未知杆菌归入这一群。     

Monitoring of the prokaryotic diversity in pit mud from a Luzhou‐flavour liquor distillery and evaluation of two predominant archaea using qPCR assays

Pit mud plays a crucial role in the production of the Luzhou‐flavour liquor. Its quality directly determines liquor quality and yield. The aged pit mud produced good quality liquor but aging pit mud did not. The aim of this work was to investigate the prokaryotic diversity of the aged and aging pit mud from a Luzhou‐flavour liquor distillery using molecular methods. Two bacteria‐specific and two archaea‐specific 16S rRNA gene clone libraries were constructed and analysed using amplified ribosomal DNA restriction analysis. A total of 273 clones were studied, which resulted in 28 operational taxonomic units. Bacteroidetes and Firmicutes predominated in both the aged and aging pit mud, but Synergistetes and Actinobacteria were only detected in the aged pit mud. The family Methanosaeta dominated in the aged pit mud, while the Methanosarcina predominated in the aging pit mud. These results were confirmed using two genus‐specific quantitative real time PCR assays. This research distinguished microbial community structure in the aged and aging pit mud for the first time, and has laid an initial foundation for identifying good quality pit mud and for maintaining the quality of pit mud. Copyright © 2014 The Institute of Brewing & Distilling     

浙江地区中温大曲贮存过程细菌群落多样性及功能预测分析

利用16S rRNA高通量测序和理化分析技术分别解析浙江地区中温大曲贮存过程中细菌群落多样性和理化特征演替,并结合生物信息学方法预测菌群功能。结果表明,大曲贮存90 d后,pH和含水量显著降低（P<0.05）,酸度显著升高（P<0.05）,且贮存前后细菌群落多样性和结构组成差异显著（P<0.05）。乳酸杆菌属（Lactobacillus）和魏斯式菌属（Weissella）是大曲贮存期间的绝对优势属,其相对丰度范围分别为47.56%～63.44%和25.16%～42.77%。魏斯式菌属相对丰度与含水量和pH呈正相关,而酸度与乳酸杆菌属、醋酸杆菌属（Acetobacter）和克罗彭斯特菌属（Kroppenstedtia）相对丰度呈正相关。此外,功能预测结果表明中温大曲贮存期间的基因功能主要与代谢、遗传信息处理和环境信息处理等功能相关。