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131.
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经对胜利油田近三年来典型井例测试资料分析评价及其增产措施效果分析认为,有三类地层压裂增油效果很好:地层物性相对较好的常压系统且存在严重堵塞的储层;物性较好的常压地层;高压低渗透储层。有两类地层酸化效果很好:灰岩、白云岩裂缝、低产低渗透储层;物性较好且存在严重污染堵塞的储层。其它地层压裂、酸化一般是无效的。 相似文献
134.
Patrick Musonda 《Computational statistics & data analysis》2008,52(4):1942-1957
Second-order expressions for the asymptotic bias and variance of the log relative incidence estimator are derived for the self-controlled case series model in a simplified scenario. The dependence of the bias and variance on factors such as the relative incidence and ratio of risk to observation period are studied. Small-sample performance of the estimator in realistic scenarios is investigated using simulations. It is found that, in scenarios likely to arise in practice, asymptotic methods are valid for numbers of cases in excess of 20-50 depending on the ratio of the risk period to the observation period and on the relative incidence. The application of Monte Carlo methods to self-controlled case series analyses is also discussed. 相似文献
135.
Current understanding of the mechanisms involved in osseointegration following implantation of a biomaterial has led to adhesion quantification being implemented as an assay of cytocompatibility. Such measurement can be hindered by intra-sample variation owing to morphological changes associated with the cell cycle. Here we report on a new scanning electron microscopical method for the simultaneous immunogold labelling of cellular focal adhesions and S-phase nuclei identified by BrdU incorporation. Prior to labelling, cellular membranes are removed by tritonization and antigens of non-interest blocked by serum incubation. Adhesion plaque–associated vinculin and S-phase nuclei were both separately labelled with a 1.4 nm gold colloid and visualized by subsequent colloid enhancement via silver deposition. This study is specifically concerned with the effects microgroove topographies have on adhesion formation in S-phase osteoblasts. By combining backscattered electron (BSE) imaging with secondary electron (SE) imaging it was possible to visualize S-phase nuclei and the immunogold-labelled adhesion sites in one energy 'plane' and the underlying nanotopography in another. Osteoblast adhesion to these nanotopographies was ascertained by quantification of adhesion complex formation. 相似文献
136.
We report here a non-enzymatic detecting electrode strip for fast monitoring of uric acid in human whole blood. A single-use amperometric uric acid sensor strip, incorporating a three-electrode configuration, has been fabricated on a polypropylene substrate using low cost screen-printing (thick-film) technology. Both the working and counter electrodes were prepared by screen-printing commercial carbon ink. The integration on the same support of pseudo-reference electrode was obtained by screen-printing a commercial silver ink and subsequent electrochemical pretreatment. Simply by placing a 20 μl human whole blood drop on the strip is enough for uric acid analysis by square-wave voltammetry. Real human whole blood samples were analyzed by this method and compared to the phosphotungstic acid clinical test procedure with satisfactory results. 相似文献
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138.
本文介绍了利用计算机技术、网络技术、三遥技术对广播电视发射技术指标进行实时自动测试的系统,以便进一步为安全优质播出提供技术保证。 相似文献
139.
Dr. C. R. Nagarajah W. Thompson 《The International Journal of Advanced Manufacturing Technology》1994,9(5):333-342
There are two aspects to cell formation in flexible manufacturing systems, cell sizing or deciding on the optimum number of machines to be allocated to each cell, and then allocation of specific machines to each cell. Although the latter problem has been investigated extensively there is a paucity of published work on the former. This paper discusses the effects of cell sizing on operational flexibility.Operational flexibility is that aspect of flexibility that enables manufacturing systems to respond with speed and efficiency to changes in the manufacturing environment while maintaining an effective level of control. 相似文献
140.
高灵敏度荧光显微镜及其在细胞光敏效应研究中的应用 总被引:1,自引:0,他引:1
研制了一种可探测极微弱细胞荧光图象的高灵敏度荧光显微镜,在仪器中使用了一个高质量的亮度增益为50,000的象增强器对细胞的荧光图象进行光能量放大,因而这种荧光显微镜可探测到普通荧光显微镜不能观察的微弱荧光图象。由于仪器中光源的输出强度很小,避免了长时间观察对活细胞的光损伤,并可观察到荧光效率低的荧光物质的分布图象。探测系统中用计算机采集图象数据,同时对数据进行必要处理,以提高图象的质量。这种高灵敏度的荧光显微镜可用于分析细胞中荧光物质的分布,以及细胞动态生理过程的研究。使用此仪器已直接观察到光敏竹红菌甲素在Hela细胞(人体宫颈癌细胞)中的分布,细胞受光照射后的光损伤现象及加入三种细胞保护剂后获得不同程度保护的细胞状态。 相似文献