Iron Mobilization from Ferritin in Yeast Cell Lysate and Physiological Implications

Most in vitro iron mobilization studies from ferritin have been performed in aqueous buffered solutions using a variety of reducing substances. The kinetics of iron mobilization from ferritin in a medium that resembles the complex milieu of cells could dramatically differ from those in aqueous solutions, and to our knowledge, no such studies have been performed. Here, we have studied the kinetics of iron release from ferritin in fresh yeast cell lysates and examined the effect of cellular metabolites on this process. Our results show that iron release from ferritin in buffer is extremely slow compared to cell lysate under identical experimental conditions, suggesting that certain cellular metabolites present in yeast cell lysate facilitate the reductive release of ferric iron from the ferritin core. Using filtration membranes with different molecular weight cut-offs (3, 10, 30, 50, and 100 kDa), we demonstrate that a cellular component >50 kDa is implicated in the reductive release of iron. When the cell lysate was washed three times with buffer, or when NADPH was omitted from the solution, a dramatic decrease in iron mobilization rates was observed. The addition of physiological concentrations of free flavins, such as FMN, FAD, and riboflavin showed about a two-fold increase in the amount of released iron. Notably, all iron release kinetics occurred while the solution oxygen level was still high. Altogether, our results indicate that in addition to ferritin proteolysis, there exists an auxiliary iron reductive mechanism that involves long-range electron transfer reactions facilitated by the ferritin shell. The physiological implications of such iron reductive mechanisms are discussed.     

碱金属盐对气基还原铁矿石的催化规律研究

通过气体催化还原氧化铁试验,研究了碱金属盐对气基还原铁氧化物反应的影响规律,对于同一种阳离子的盐催化活性规律为:氟化物＞氯化物＞碘化物＞溴化物;硝酸盐＞氢氧化物＞碳酸盐＞硫酸盐>磷酸盐.并提出了一种新的微观催化机理:添加剂的催化作用在于添加剂的原子改变Fe-O化学键的作用程度;添加剂的催化活性与阳离子、阴离子种类以及化合物的稳定性有关;这三方面因素相互耦合,共同决定添加剂的催化性能;其催化性能用公式表示为:Ψαf(M ) βf(X-)-γ(T)f(MX).     

金属型球铁磨球非平衡凝固特性与成分控制

通过分析不同成分球铁磨球零件性能和金相组织,对金属型磨球的非平衡凝固特性进行了较深入的研究.试验结果发现,过共晶球铁凝固时出现非平衡奥氏体;亚共晶球铁出现非平衡渗碳体相和晕圈铁素体;在柱状晶与等轴晶过渡区中存在着夹杂和偏聚物.为改善球铁磨球的显微组织及性能,球铁成分应控制在共晶或稍过共晶范围.     

武钢3200 m3高炉出铁场设计

结合武钢5号、6号、7号3座3200m^3高炉出铁场设计实践,对3200m^3高炉风口平台及出铁场设计的工艺布置及平台结构形式、设备选型（包括炉前吊车、泥炮、开铁口机及揭、移盖机等）、出铁场除尘抽风量及其分配等进行了阐述。     

铁矿石的烧结基础特性之新概念

长期以来,人们对烧结用铁矿石的评价主要集中在化学成分、粒度组成、矿物特征等常温性能方面,而对铁矿石在烧结过程中的高温行为和作用知之甚少.根据对这一问题的深入思考和实验室的基础研究,提出了\     

固溶淬火钨镍铁合金力学性能与镍铁比关系

研究了固溶淬火条件下Ni/Fe比（质量分数）对W-Ni-Fe高密度钨合金力学性能的影响规律．拉伸测试表明．同溶淬火条件下W-Ni-Fe合金的最佳Ni-Fe比发生偏移,随Ni/Fe比增加,钨合金的强度和韧性同步提高,当镍铁比达到9/1时强度和韧性都达到最大值,其后合金的强韧性随Ni-Fe比增加而同步降低．通过X射线和TEM分析发现,缓冷条件下W-Ni-Fe合金中有脆性β相析出,固溶淬火工艺能有效抑制β相析出．     

Effects of land use and changes in cover on the transformation and transportation of iron:A case study of the Sanjiang Plain,Northeast China

Wetland reclamation has been ongoing in the Sanjiang Plain since the mid-1950s,which has resulted in major changes in wetlands and the agriculture ecosystem in the region that have influenced the iron output to the Sea of Okhotsk and limited the primary productivity in the North Pacific Ocean.This study was conducted to investigate the chemical forms of iron in different aquatic environments(agricultural water including groundwater,paddy water,and canal water;wetland water including marsh water,marsh stream...     

三维软件SolidWorks在铁包倾翻力矩计算中的应用

铁包倾翻力矩计算在炼钢工艺流程设计中有重要的意义和作用,对倾翻过程中的力矩进行核算,有利于了解副钩的工作过程.通过利用Solidworks三维软件对某工程100 t铁包进行三维建模,用其质量特性查询功能可得出铁水包的重量及重心位置等物理参数,以提供力矩计算所需数据.     

巨型球体钢结构工程焊接技术

根据安装顺序,严格执行焊接程序,消化创新了“鸟巢”钢结构合龙技术,实现了分层错45°合龙线的均匀布置,使合龙进展顺利;特别是根据主结构“统一对称、分区进行;自内而外、隔层焊接;分层合龙”的思想,焊接程序应当是:第一步,以柱为点,自内而外,分区进行,对称焊接;第二步,以梁为主,自内而外,先环后径,分区对称,逐步合龙;注意...     

过筛煤粉在铸铁件湿型砂铸造生产中的作用

正确选择煤粉,其性能要求同时具有低灰分、高挥发分和高膨胀指数,以及具有合适的筛分粒度这一关键因素,以确保所生产的铸件没有机械粘砂缺陷和表面质量问题.通过仔细选择、安全操作和加工处理,煤粉仍然是一个优良的、简易的、经济的有效解决方案.煤粉在储存、加工和运输过程中易于调节,然后经过进一步的安全处理,最终被铸造厂使用.尽管煤粉有诸多不足之处,对于最终用户来说,它仍然是经济合算的.而且现代加工和筛分方法确保它在最佳条件下使用.