In order to reduce the shrinkage porosity of nickel-based superalloy castings in the investment casting process, the effects of different gating systems on mold filling, solidification process, and prediction of shrinkage porosity of aero-engine turbine nozzle castings were investigated by simulation and experimental methods. Results show that the design of the vertical runner would cause greater turbulence of the melt in the riser during the mold filling process, and the outer runner is not necessary. With the decrease in number of runners, the hot spot moves down towards the casting, and the shrinkage and porosity defects are formed in the casting below the riser. In the original designs, a certain tendency of shrinkage and porosity defect is found in the vanes, inner rings, and outer rings of the castings by both simulation prediction and experiment. Finally, based on the processing optimization, the aero-engine turbine nozzle casting with no shrinkage and porosity defects is obtained.