Carbon nanotube-tipped endoscope for in situ intracellular surface-enhanced Raman spectroscopy

Gold nanoparticle-decorated carbon nanotubes (CNTs) are used to study intracellular environments in situ using surface-enhanced Raman spectroscopy (SERS). CNTs are decorated with gold nanoparticles and assembled onto the tips of pulled glass capillaries to form a SERS-enabled endoscope. The sub-micrometer size and high mechanical strength of the endoscope make it possible to penetrate the cell membrane for intracellular probing and remain positioned inside during lengthy SERS measurements without causing damage to the cell. Using the SERS-enabled endoscope, DNA and other biomolecules are detected in situ within the nucleus of a single human cervical carcinoma cell in a minimally invasive manner. The SERS-enabled endoscopes exhibit high selectivity and sensitivity for detecting trace amounts of analytes (≈1 pM) in biofluid environments, highlighting their capabilities as label-free, biological sensors for real-time in situ cellular diagnostics, biological detection, and pharmaceutical research.     

牛奶中阿莫西林含量表面增强拉曼光谱检测方法的建立

以香水百合花瓣绿色合成的银溶胶为表面增强拉曼散射（SERS）活性基底，建立了牛奶中阿莫西林含量的SERS检测方法。对SERS检测条件进行了优化，考察了银溶胶浓度、银溶胶与阿莫西林溶液的体积比和氯化钠溶液的添加与否对SERS检测的影响。试验结果表明：当银溶胶浓度为106.04 μg/mL、银溶胶与阿莫西林溶液的体积比为41且不添加氯化钠的条件下，获得的SERS信号最强。在该条件下，阿莫西林标准溶液的浓度与1 055 cm^-1 处拉曼特征峰的SERS信号强度在1～1 000 μg/mL 范围内有良好的线性关系，方法的最低检出浓度可达到1 μg/mL。3份加标牛奶中阿莫西林的加标回收率为84.51%～93.62%，相对标准偏差（RSD）为5.16%。该方法简单方便，可实现牛奶中一定浓度阿莫西林的检测。     

Diamond-like carbon thin films produced by femtosecond pulsed laser deposition of fullerite

Diamond-like carbon films have been deposited from a fullerite target by ultra-short pulsed laser deposition technique. The results indicate that the films morphology and structure, determined by scanning electron microscopy, atomic force microscopy and energy dispersive X-ray diffraction, depend strongly on the substrate temperature. X-ray photoelectron, X-ray Auger electron, Raman and surface enhanced Raman scattering spectra indicate that the fs-DLC films composition involves a mixed sp, sp² and sp³ carbon network consisting of aromatic rings and sp³ diamond-like structures linked by chains of different lengths and composition. The films deposited at room temperature, presenting the higher content of sp³ carbon (48%), also contain C₆₀ crystalline phase and show a very high hardness of 49 GPa.     

Surface-enhanced Raman scattering substrate of silver nanoparticles depositing on AAO template fabricated by magnetron sputtering

In this report, we describe a fabrication process of low-cost and highly sensitive SERS substrates by using a simple anodizing setup and a low-energy magnetron sputtering method. The structure of the SERS substrates consists of silver nanoparticles deposited on a layer of anodic aluminum oxide (AAO) template. The fabricated SERS substrates are investigated by a scanning electron microscope (SEM), a transmission electron microscope (TEM), and a confocal Raman spectroscope. We have verified from the surface morphology that the fabricated SERS substrates consist of high-density round-shape silver nanoparticles where their size distribution ranges from 10 to 30 nm on the top and the bottom of nanopores. The surface-enhanced Raman scattering activities of these nanostructures are demonstrated using methylene blue (MB) as probing molecules. The detection limit of 10⁻⁸ M can be achieved from this SERS substrate.     

苯并三氮唑与4—羟基苯并三氮唑在氯化钠溶液中对铜的缓蚀作用

用表面增强拉曼光谱技术（SERS）对在3％NaCl溶液中苯并三氮唑（BTA）及其衍生物4－羟基苯并三氮唑（4CBTA）对铜的缓蚀作用机理进行了研究,发现4CBTA对铜的缓蚀作用机理与BTA相似,在较正电位下两者都是通过三唑环与铜形成配合物覆盖在铜表面随着电位负移,铜电极表面吸附的分子形式的BTA或4CBAT数量增多;4CBT中的－COOH基团只是起到空间位阻的作用,没有参与电极表面的吸附,两者复配使用时以BTA吸附为主,其缓蚀机理没有发生改变。也没有产生协同效应。     

磁性Fe⁃Au复合材料的制备及SERS性能

表面增强拉曼散射光谱(SERS)是一种广泛应用于低浓度分子物种识别并且能够提供结构信息的技术。SERS检测中基底纳米材料的颗粒大小、形状和空间分布对检测结果有着重要影响。探索制备新型纳米结构可以为SERS研究和应用提供新的活性基底和理论基础。由于具有良好的传质和吸附性能,多孔材料广泛应用于构筑SERS活性基底纳米复合物。以多孔磁性纳米材料作固相载体,通过水热合成多孔纳米α⁃Fe2O3,再将其还原成Fe,使Fe的表面也有孔道存在。在Fe表面自组装Au纳米材料构筑SERS基底,以对巯基苯胺（PATP）和三聚氰胺水溶液作为目标分子,对其SERS性能进行测试。调控Au纳米粒子尺寸大小,考察其对SERS性能影响,得到50 nm Au具有最优性能。利用扫描电子显微镜（SEM）、X 射线衍射(XRD)和N2吸附等表征手段,对纳米复合物结构及组成进行分析表征。结果表明,得到了一种同时具有吸附和检测效果的纳米复合物SERS基底。