输电线路设计阶段如何控制工程造价

以设计的二滩-石板箐500 KV送电线路工程为例,从线路本体工程出发,分析在工程设计阶段如何控制工程造价。     

LR20/LR14电池兼容生产线的工艺探讨

介绍了LR20L/R14碱性锌锰电池兼容生产线设备及工艺特点,讨论了通过工艺改进,实现同一套设备可兼容生产两种型号的碱锰电池。实验数据表明,4个正极环结构的LR20电池其放电性能有所提高。     

配电网线路开断后潮流的新算法

在配电网回路阻抗法潮流算法的基础上,提出了一种新的配电网线路开断后潮流算法:对应取零法.该算法通过对已有的基态潮流方程的矩阵经过适当的变形,得到所求的因子表,从而节约了大量计算机时.对一个35节点配电网实例计算与比较的结果证明了"对应取零法"的可行性和正确性.     

The Deubiquitinase OTUB1 Is a Key Regulator of Energy Metabolism

Dysregulated energy metabolism is a major contributor to a multitude of pathologies, including obesity and diabetes. Understanding the regulation of metabolic homeostasis is of utmost importance for the identification of therapeutic targets for the treatment of metabolically driven diseases. We previously identified the deubiquitinase OTUB1 as substrate for the cellular oxygen sensor factor-inhibiting HIF (FIH) with regulatory effects on cellular energy metabolism, but the physiological relevance of OTUB1 is unclear. Here, we report that the induced global deletion of OTUB1 in adult mice (Otub1 iKO) elevated energy expenditure, reduced age-dependent body weight gain, facilitated blood glucose clearance and lowered basal plasma insulin levels. The respiratory exchange ratio was maintained, indicating an unaltered nutrient oxidation. In addition, Otub1 deletion in cells enhanced AKT activity, leading to a larger cell size, higher ATP levels and reduced AMPK phosphorylation. AKT is an integral part of insulin-mediated signaling and Otub1 iKO mice presented with increased AKT phosphorylation following acute insulin administration combined with insulin hypersensitivity. We conclude that OTUB1 is an important regulator of metabolic homeostasis.     

江门市区10 kV架空线路雷击分析和防治措施

以江门市区10kV架空线路雷击率为研究对象,以架空线路年雷击率计算理论为基础,对年均公里雷击率理论值与实际值进行比较分析。分析结果表明,市区裸导线的年均公里雷击率低于理论值的50%,绝缘导线的年均公里雷击率基本接近理论值,各镇线路的年均公里雷击率都超过理论值。最后针对存在的问题,提出了有效防雷措施和方法。     

Dipotassium Glycyrrhizinate on Melanoma Cell Line: Inhibition of Cerebral Metastases Formation by Targeting NF-kB Genes-Mediating MicroRNA-4443 and MicroRNA-3620—Dipotassium Glycyrrhizinate Effect on Melanoma

Glycyrrhizic acid (GA), a natural compound isolated from licorice (Glycyrrhiza glabra), has exhibited anti-inflammatory and anti-tumor effects in vitro. Dipotassium glycyrrhizinate (DPG), a dipotassium salt of GA, also has shown an anti-tumor effect on glioblastoma cell lines, U87MG and T98G. The study investigated the DPG effects in the melanoma cell line (SK-MEL-28). MTT assay demonstrated that the viability of the cells was significantly decreased in a time- and dose-dependent manner after DPG (IC₅₀ = 36 mM; 24 h). DNA fragmentation suggested that DPG (IC₅₀) induced cellular apoptosis, which was confirmed by a significant number of TUNEL-positive cells (p-value = 0.048) and by PARP-1 [0.55 vs. 1.02 arbitrary units (AUs), p-value = 0.001], BAX (1.91 vs. 1.05 AUs, p-value = 0.09), and BCL-2 (0.51 vs. 1.07 AUs, p-value = 0.0018) mRNA compared to control cells. The proliferation and wound-healing assays showed an anti-proliferative effect on DPG-IC₅₀-treated cells, also indicating an inhibitory effect on cell migration (p-values < 0.001). Moreover, it was observed that DPG promoted a 100% reduction in melanospheres formation (p-value = 0.008). Our previous microRNAs (miRs) global analysis has revealed that DPG might increase miR-4443 and miR-3620 expression levels. Thus, qPCR showed that after DPG treatment, SK-MEL-28 cells presented significantly high miR-4443 (1.77 vs. 1.04 AUs, p-value = 0.02) and miR-3620 (2.30 vs. 1.00 AUs, p-value = 0.01) expression compared to control cells, which are predicted to target the NF-kB, CD209 and TNC genes, respectively. Both genes are responsible for cell attachment and migration, and qPCR revealed significantly decreased CD209 (1.01 vs. 0.54 AUs, p-value = 0.018) and TNC (1.00 vs. 0.31 AUs, p-value = 2.38 × 10⁻⁶) mRNA expression levels after DPG compared to untreated cells. Furthermore, the migration of SK-MEL-28 cells stimulated by 12-O-tetradecanoylphorbol-13-acetate (TPA) was attenuated by adding DPG by wound-healing assay (48 h: p-value = 0.004; 72 h: p-value = 7.0 × 10⁻⁴). In addition, the MMP-9 expression level was inhibited by DPG in melanoma cells stimulated by TPA and compared to TPA-treated cells (3.56 vs. 0.99 AUs, p-value = 0.0016) after 24 h of treatment. Our results suggested that DPG has an apoptotic, anti-proliferative, and anti-migratory effect on SK-MEL-28 cells. DPG was also able to inhibit cancer stem-like cells that may cause cerebral tumor formation.     

Glioblastoma Extracellular Vesicle-Specific Peptides Inhibit EV-Induced Neuronal Cytotoxicity

WHO Grade 4 IDH-wild type astrocytoma (GBM) is the deadliest brain tumor with a poor prognosis. Meningioma (MMA) is a more common “benign” central nervous system tumor but with significant recurrence rates. There is an urgent need for brain tumor biomarkers for early diagnosis and effective treatment options. Extracellular vesicles (EVs) are tiny membrane-enclosed vesicles that play essential functions in cell-to-cell communications among tumor cells. We aimed to identify epitopes of brain tumor EVs by phage peptide libraries. EVs from GBM plasma, MMA plasma, or brain tumor cell lines were used to screen phage-displayed random peptide libraries to identify high-affinity peptides. We purified EVs from three GBM plasma pools (23 patients), one MMA pool (10 patients), and four brain tumor cell lines. We identified a total of 21 high-affinity phage peptides (12 unique) specific to brain tumor EVs. The peptides shared high sequence homologies among those selected by the same EVs. Dose–response ELISA demonstrated that phage peptides were specific to brain tumor EVs compared to controls. Peptide affinity purification identified unique brain tumor EV subpopulations. Significantly, GBM EV peptides inhibit brain tumor EV-induced complement-dependent cytotoxicity (necrosis) in neurons. We conclude that phage display technology could identify specific peptides to isolate and characterize tumor EVs.     

架空输电线路环境荷载响应与设计理论的研究

我国地域广阔, 气候跨区域变化较大, 地形地貌复杂多变, 各地区风、雨以及导线覆冰的分布规律千差万别。由于目前环境荷载因素对输电塔线结构的严重破坏频繁发生, 造成了巨大的经济损失。因此, 有必要建立输电塔线结构环境荷载响应分析理论和进行设计优化, 提高其结构设计的规范水准, 并加强灾害监测控制。这对减轻环境荷载造成的输电塔线结构的灾害具有不可估量的作用。     

变截面导弹发射筒成形工艺研究

对变截面导弹发射筒的缠绕工艺进行了研究。目的在于简化工艺,提高精度,减轻质量,节约成本,缩短生产周期。详细地介绍了这种发射筒的成形工艺选择、芯模设计、准备工艺设计、缠绕线形设计以及缠绕工艺设计,使这种发射筒满足武器系统提出的新要求。     

A test object with a line width less than 10 nm for scanning electron microscopy

Data are given on the test object for scanning electron microscopy in which the components (ridges) are of trapezoidal profile and have large angles of inclination for the side walls. The width of the top surface of a projection is less than 10 nm. Methods have been developed for measuring such dimensions of test objects on standard scanning electron microscopes. __________ Translated from Izmeritel'naya Tekhnika, No. 8, pp. 20–23, August, 2008.