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91.
通过跟踪含有液泡前体特异标记物的组分, 利用细胞壁酶水解胞壁制造原生质体, 并用真针头挤压的方法裂解细胞, 用蔗糖密度梯度离心的方法分离液泡前体. 通过相差显微镜观察, 发现所分离的液泡前体保持完整的结构. 利用不同细胞器的标记物抗体进行免疫标记检测, 证明所纯化的液泡前体不含其他细胞器, 具有较高的纯度. 相似文献
92.
Iwao Shimizu Yuji Naito Iwao Yamaguchi Katsuyuki Kaiho Hitoshi Mizoguchi Satoru Yanabu 《Electrical Engineering in Japan》2008,164(1):52-61
Using conventional high‐temperature superconducting wire, a model superconducting fault current limiter (SFCL) is made and tested. Solenoid coil using Bi2223 silver sheath wire is so made that inductance is as small as possible and a vacuum interrupter is connected in series to it. A conventional reactor coil is connected in parallel. When the fault current flows in this equipment, superconducting wire is quenched and current is transferred into the parallel coil because of voltage drop of superconducting wire. This large current in parallel coil actuates magnetic repulsion mechanism of vacuum interrupter. Due to opening of vacuum interrupter, the current in superconducting wire is broken. By using this equipment, current flow time in superconducting wire can be easily minimized. On the other hand, the fault current is also easily limited by large reactance of parallel coil. © 2008 Wiley Periodicals, Inc. Electr Eng Jpn, 164(1): 52–61, 2008; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20315 相似文献
93.
94.
提出一种配电网电容电流测量的新方法,在零序电压互感器的开口三角侧串联一个可调电感,通过注入一个变频恒流信号寻找配电网的谐振频率,改变可调电感的数值后寻找另一谐振频率,联立2个谐振方程求解电容电流,该方法消除了电压互感器漏抗对测量的影响。开发了配电网电容电流测量仪,经模拟试验和现场测试表明,该方法具有安全、快捷、准确等特点,适用于中性点不接地或经随调式消弧线圈接地配电网。 相似文献
95.
This paper presents the mass transfer results from an impinging liquid jet to a rotating disk. The mass transfer coefficients were measured using the electrochemical limiting diffusion current technique (ELDCT). Rotational Reynolds number (Rer) in the range of 3.4 × 104–1.2 × 105, jet Reynolds number (Rej) 1.7 × 104–5.3 × 104 and non-dimensional jet-to-disk spacing (H/d) 2–8 were taken into consideration as parameters. It was found that the jet impingement resulted in a substantial enhancement in the mass transfer compared to the case of the rotating disk without jet. 相似文献
96.
97.
The influence of raw material characteristics on the industrial pelletizing process and pellet quality 总被引:1,自引:0,他引:1
Mehrdad Arshadi Rolf Gref Paul Geladi Sten-Axel Dahlqvist Torbjörn Lestander 《Fuel Processing Technology》2008
Industrial pelletizing of sawdust was carried out as a designed experiment in the factors: sawdust moisture content, fractions of fresh pine, stored pine and spruce. The process parameters and response variables were energy consumption, pellet flow rate, pellet bulk density, durability and moisture content. The final data consisted of twelve industrial scale runs. Because of the many response variables, data evaluation was by principal component analysis of a 12 × 9 data matrix. The two principal component model showed a clustering of samples, with a good reproducibility of the center points. It also showed a positive correlation of energy consumption, bulk density and durability all negatively correlated to flow rate and moisture content. The stored pine was more related to high durability and bulk density. The role of the spruce fraction was unclear. The design matrix, augmented with the process parameters was a 12 × 6 matrix. Partial least squares regression showed excellent results for pellet moisture content and bulk density. The model for durability was promising. A 12 × 21 data matrix of fatty- and resin acid concentrations measured by GC–MS showed the differences between fresh and stored pine very clearly. The influence of the spruce fraction was less clear. However, the influence of the fatty- and resin acids on the pelletizing process could not be confirmed, indicating that other differences between fresh and stored pine sawdust have to be investigated. This work shows that it is possible to design the pelletizing process for moderate energy consumption and high pellet quality. 相似文献
98.
O. Gravrand E. De Borniol S. Bisotto L. Mollard G. Destefanis 《Journal of Electronic Materials》2007,36(8):981-987
This paper aims at studying the feasibility of very long infrared wavelength (VLWIR) (12–18 μm) focal plane arrays using n-on-p planar ion-implanted technology. To explore and analyze the feasibility of such VLWIR detectors, a set of four Cd
x
Hg1−x
Te LPE layers with an 18 μ cutoff at 50 K has been processed at Defir (LETI/LIR–Sofradir joint laboratory), using both our “standard” n-on-p process and our improved low dark current process. Several 320 × 256 arrays, 30-μm pitch, have been hybridized on standard Sofradir readout circuits and tested. Small dimension test arrays characterization
is also presented. Measured photonic currents with a 20°C black body suggest an internal quantum efficiency above 50%. Typical
I(V) curves and thermal evolution of the saturation current are discussed, showing that standard photodiodes remain diffusion
limited at low biases for temperatures down to 30 K. Moreover, the dark current gain brought by the improved process is clearly
visible for temperatures higher than 40 K. Noise measurements are also discussed showing that a very large majority of detectors
appeared background limited under usual illumination and biases. In our opinion, such results demonstrate the feasibility
of high-performance complex focal plane arrays in the VLWIR range at medium term. 相似文献
99.
100.
3-D MCM封装技术及其应用 总被引:1,自引:0,他引:1
介绍了超大规模集成电路(VLSI)用的3-D MCM封装技术的最新发展,重点介绍了3-D MCM封装垂直互连工艺,分析了3-D MCM封装技术的硅效率、复杂程度、热处理、互连密度、系统功率与速度等问题,并对3-D MCM封装的应用作了简要说明。 相似文献