基于RGD多肽掺杂聚吡咯-铟锡氧化物的仿生微电极构建及用于细胞生物学行为的电化学阻抗谱检测

构建一种基于RGD多肽分子掺杂聚吡咯膜修饰的铟锡氧化物微电极(PPy/RGD-ITO),并以此作为传感电极实现细胞生物学行为的电化学阻抗谱检测.采用光刻技术蚀刻感光干膜绝缘层制备ITO微电极;以含RGD模体的多肽分子作为吡咯电聚合唯一的掺杂阴离子,通过电化学共聚合方式在ITO微电极表面沉积PPy/RGD复合膜形成PPy/RGD-ITO微电极;原子力显微镜(AFM)、接触角测量仪和傅里叶变换红外光谱仪(FTIR)分别表征PPy/RGD复合膜的表面拓扑形貌、湿润性和组成成分;人肺癌细胞株A549铺展、粘附及增殖实验考察了PPy/RGD复合膜与细胞间的相互作用;以构建的PPy/RGD-ITO微电极作为传感电极,通过电化学阻抗谱技术对A549细胞粘附增殖行为及天然抗癌药物分子重楼皂苷I的细胞毒性进行了分析.结果显示,通过简单的电化学共聚合成功将RGD分子掺杂进PPy膜内,且PPy/RGD复合膜具有优异的表面物理性能;PPy基质膜内掺杂的RGD分子保留其生物活性,相比裸ITO电极和聚4-苯乙烯磺酸钠(PSS)掺杂的PPy膜,PPy/RGD复合膜能更好地促进A549细胞的铺展、粘附和增殖;由于PPy/RGD-ITO微电极表面A549细胞形态学变化可改变电极系统的阻抗谱特征,因此通过电化学阻抗谱技术可解析A549细胞粘附增殖行为学信息,同时可定量分析重楼皂苷I细胞毒性.因此,通过简单的电化学共聚合方法将生物活性RGD分子掺杂进PPy膜内制备出的PPy/RGD膜具有优良的生物相容性,可作为一种重要的仿生电极修饰材料用于构建电子系统和细胞生物学系统的耦合界面,未来可应用于细胞生物学行为及药物筛选研究.     

基于NaOH刻蚀玻碳电极的电化学传感器在检测膀胱癌DNA中的应用

制备了一种基于活化的玻碳电极的新型电化学DNA生物传感器,可用于膀胱癌DNA的检测.通过循环伏安法(CV)实现玻碳电极在NaOH溶液中的刻蚀,使电极表面负载大量官能团,为DNA提供连接位点,由Laviron方程计算得到玻碳电极表面的羧基浓度为 1.022×10-6 mol/cm2.亚甲基蓝(MB)作为电化学检测的杂交指示剂.采用原子力显微镜(AFM)对刻蚀后的电极进行了形貌表征.在最优杂交条件下,通过差分脉冲法(DPV)计算出最佳检测限为5.677×10-13 mol/L(n=5),适用目标 DNA浓度范围1×10-8 mol/L~1×10-12 mol/L.该传感器有望用于实际样品中膀胱癌DNA的快速检测.     

Flexible coloring

Motivated by reliability considerations in data deduplication for storage systems, we introduce the problem of flexible coloring. Given a hypergraph H and the number of allowable colors k, a flexible coloring of H is an assignment of one or more colors to each vertex such that, for each hyperedge, it is possible to choose a color from each vertex?s color list so that this hyperedge is strongly colored (i.e., each vertex has a different color). Different colors for the same vertex can be chosen for different incident hyperedges (hence the term flexible). The goal is to minimize color consumption, namely, the total number of colors assigned, counting multiplicities. Flexible coloring is NP-hard and trivially approximable, where s is the size of the largest hyperedge, and n is the number of vertices. Using a recent result by Bansal and Khot, we show that if k is constant, then it is UGC-hard to approximate to within a factor of s−ε, for arbitrarily small constant ε>0. Lastly, we present an algorithm with an approximation ratio, where k^′ is number of colors used by a strong coloring algorithm for H.     

Consensus models: Computational complexity aspects in modern approaches to the list coloring problem

In the paper we study new approaches to the problem of list coloring of graphs. In the problem we are given a simple graph G=(V,E) and, for every v∈V, a nonempty set of integers S(v); we ask if there is a coloring c of G such that c(v)∈S(v) for every v∈V. Modern approaches, connected with applications, change the question—we now ask if S can be changed, using only some elementary transformations, to ensure that there is such a coloring and, if the answer is yes, what is the minimal number of changes. In the paper for studying the adding, the trading and the exchange models of list coloring, we use the following transformations:

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adding of colors (the adding model): select two vertices u, v and a color c∈S(u); add c to S(v), i.e. set S(v):=S(v)∪{c};

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trading of colors (the trading model): select two vertices u, v and a color c∈S(u); move c from S(u) to S(v), i.e. set S(u):=S(u)?{c} and S(v):=S(v)∪{c};

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exchange of colors (the exchange model): select two vertices u, v and two colors c∈S(u), d∈S(v); exchange c with d, i.e. set S(u):=(S(u)?{c})∪{d} and S(v):=(S(v)?{d})∪{c}.

Our study focuses on computational complexity of the above models and their edge versions. We consider these problems on complete graphs, graphs with bounded cyclicity and partial k-trees, receiving in all cases polynomial algorithms or proofs of NP-hardness.     

Ca2+和Pb2+与双层类脂膜相互作用的研究

用电化学方法研究了卵磷脂和氧化胆固醇构成的双层类脂膜与 Ca~(2+)和 Pb~(2+)的相互作用。结果发现:双层类脂膜与含 Ca~(2+)或 Pb~(2+)的溶液接触15分钟后,产生较稳定的膜电位信号并与溶液中 Ca~(2+),Pb~(2+)浓度相关。其作用机制主要是源于静电作用与合作效应,同时,对三种不同存在状态的双层类脂膜的通透性进行了验证,对某些干扰离子和 pH 值的影响进行了研究。     

锡盐着色膜颜色与氧化膜中Sn含量的关系

对锡盐电解着色膜的颜色深浅与氧化膜中Ｓｎ含量两者之间的关系进行了研究。此外还探讨了锡盐电解着色液中镍离子和亚铁离子在电解着色过程中所起的作用。     

渗铬P110钢在CO2饱和模拟油田采出液中的耐蚀性

为了提高P110油套管钢的表面性能,以满足其在油田环境下服役的要求,采用包埋法对P110钢进行渗铬处理.借助扫描电镜、能谱仪和x射线衍射仪,对渗铬层的表面形貌、显微组织、成分分布和相结构进行了分析和表征,利用电化学测试技术测定P110钢及其渗铬层在CO₂饱和模拟油田采出液中的开路电位、极化曲线和交流阻抗谱.结果表明：渗铬层由Cr₂₃C₆、Cr₇C₃和（Cr,Fe）₇ C₃等相构成,且均匀、连续,与P110钢相比,具有更高的开路电位和电荷转移电阻及更低的电流密度和腐蚀速率.渗铬处理显著提高了P110钢的耐蚀性.     

Improving corrosion resistance of Q235 steel by Ni-Cr alloyed layer

Ni-Cr alloyed layer was formed on surface of Q235 steel by double glow plasma surface metallurgy to improve the corrosion resistance of substrate. The composition and microstructure of alloyed layer was analyzed by SEM and XRD. Potentiodynamic polarization and electrochemical impedance spectroscopy was applied to evaluate the corrosion resistance of the alloyed layer. The results showed working pressure had a great effect on structure of Ni-Cr alloyed layer, and the dense and smooth alloyed layer was prepared at 50 Pa working pressure. Compared with substrate, Ni-Cr alloyed layer exhibited higher corrosion potential, lower corrosion current density and larger charge transfer resistance, which indicated that Ni-Cr alloyed layer significantly modified the corrosion resistance of Q235 steel.     

锂离子电池负极材料 Na2Li2Ti6O14的嵌脱锂过程动力学研究

为了研究钛酸钠锂(Na2Li2Ti6O14)负极材料嵌脱锂的动力学行为,用溶胶-凝胶法合成 Na2Li2Ti6O14负极材料,采用 X 射线衍射法(XRD)和电子显微镜(SEM)分别对材料进行物相分析和微观形貌的观察.采用恒流充放电测试、循环伏安法(CV)和恒电流间歇滴定法(GITT)研究了 Na2Li2Ti6O14的电化学性能和嵌脱锂过程动力学.研究结果表明,制备的 Na2Li2Ti6O14材料纯度高,结晶度良好,循环稳定性好;由不同扫描速率的循环伏安法测出的 Na2Li2Ti6O14中锂离子在氧化、还原峰对应的化学扩散系数 Da和 Dc分别为7.3×10-11和7.8×10-11cm2/s;由恒电流间歇滴定技术测得的锂离子在 Na2Li2Ti6O14电极中的扩散系数为10-11~10-8cm2/s.