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31.
Quantitative Proteomics Study Reveals Changes in the Molecular Landscape of Human Embryonic Stem Cells with Impaired Stem Cell Differentiation upon Exposure to Titanium Dioxide Nanoparticles
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Lei Pan Yew Mun Lee Teck Kwang Lim Qingsong Lin Xiuqin Xu 《Small (Weinheim an der Bergstrasse, Germany)》2018,14(23)
The increasing number of nanoparticles (NPs) being used in various industries has led to growing concerns of potential hazards that NP exposure can incur on human health. However, its global effects on humans and the underlying mechanisms are not systemically studied. Human embryonic stem cells (hESCs), with the ability to differentiate to any cell types, provide a unique system to assess cellular, developmental, and functional toxicity in vitro within a single system highly relevant to human physiology. Here, the quantitative proteomics approach is adopted to evaluate the molecular consequences of titanium dioxide NPs (TiO2 NPs) exposure in hESCs. The study identifies ≈328 unique proteins significantly affected by TiO2 NPs exposure. Proteomics analysis highlights that TiO2 NPs can induce DNA damage, elevated oxidative stress, apoptotic responses, and cellular differentiation. Furthermore, in vivo analysis demonstrates remarkable reduction in the ability of hESCs in teratoma formation after TiO2 NPs exposure, suggesting impaired pluripotency. Subsequently, it is found that TiO2 NPs can disrupt hESC mesoderm differentiation into cardiomyocytes. The study unveils comprehensive changes in the molecular landscape of hESCs by TiO2 NPs and identifies the impact which TiO2 NPs can have on the pluripotency and differentiation properties of human stem cells. 相似文献
32.
Zhen EY Berna MJ Jin Z Pritt ML Watson DE Ackermann BL Hale JE 《Proteomics. Clinical applications》2007,1(7):661-671
Heart fatty acid binding protein (Fabp3) is a cytosolic protein expressed primarily in heart, and to a lesser extent in skeletal muscle, brain, and kidney. During myocardial injury, the Fabp3 level in serum is elevated rapidly, making it an ideal early marker for myocardial infarction. In this study, an MS‐based selected reaction monitoring method (LC‐SRM) was developed for quantifying Fabp3 in rat serum. Fabp3 was enriched first through an immobilized antibody, and the protein was digested on beads directly. A marker peptide of Fabp3 was quantified using LC‐SRM with a stable isotope‐labeled peptide standard. For six quality control samples with Fabp3 ranging from 0.256 to 25 ng, the average recovery following the procedure was about 73%, and the precision (%CV) between replicates was less than 7%. The Fabp3 concentrations in rat serum peaked 1 h after isoproterenol treatment, and returned to baseline levels 24 h after the dose. Elevated Fabp3 levels were also detected in rats administered with a PPAR α/δ agonist, which has shown to cause skeletal muscle necrosis. Fabp3 can be used as a biomarker for both cardiac and skeletal necroses. The cross‐validation of the LC‐SRM method with an existing ELISA method is described. 相似文献
33.
Defensive secretion produced by adult males and females ofLeptocorisa oratorius, Fabricius (Hemiptera: Coreidae) living on the host plant,Oriza sativa, was analyzed by a combined gas chromatography-mass spectroscopy technique. Both male and female secretions consisted of two major components: (E)-2-octenal andn-octyl acetate, 76% and 16% (w/w), respectively. The remaining 8% were trace compounds, some of which were identified as hexyl acetate, 3-octenal, 1-octanol, and (Z)-3-octenyl acetate. In a survey among 38 coreid defensive secretions, (E)-2-octenal andn-octyl acetate were found to occur rarely in addition to coreid-specific compounds, while their combination as primary constituents was found to be unique. Toxicity and repellency of this secretion were evaluated using two household pests,Anoplolepis longipes andSitotroga cerealella, as test insects, and lethal concentration (LC50) values of 0.24 ppm and 0.14 ppm, respectively, and repellencies of 63% and 58%, respectively, were obtained. Comparing the above values with those of a pentatomid bug,Coridius janus, evaluated under the same conditions, it was apparent that this secretion has potential as a repellent to enemies ofL. oratorius but not as a toxicant to attack them. Bioassay on the alarm activity of this secretion revealed that it elicits alarm responses, alerting and dispersing aggregated male and femaleL. oratorius: this is followed by self-coating activities. In addition, some unique behaviors were also noted among alarmedL. oratorius. 相似文献
34.
化学工业固体废物毒性分析研究 总被引:1,自引:0,他引:1
介绍了对硫酸烧渣和磷石膏两种固体废物进行急性毒性和浸出毒性鉴别试验的过程与结果,并对磷石膏由危险废物转变为非危险废物的原因进行了探讨,为硫酸烧渣和磷石膏的处置和利用提供了依据. 相似文献
35.
陶令峰 《武汉工程大学学报》2013,35(2):48-50,56
为避免使用醇类溶剂精制双磷酸盐类药物易导致的基因毒性风险和溶剂化合物问题,探讨双磷酸盐类药物的非醇类溶剂精制工艺.采用非醇类溶剂(丙酮和甲基异丁酮)分别对伊班膦酸钠和帕米磷酸钠进行精制,通过滴定法、气相色谱法(GC)等手段测定了精制前后亚磷酸盐含量及产物的残留溶剂.结果表明,采用丙酮和甲基异丁酮的精制效果明显,其亚磷酸盐含量明显下降,残留溶剂均小于5000mg/kg,丙酮和甲基异丁酮作为双磷酸盐类药物的精制溶剂具有较好的开发和应用价值. 相似文献
36.
环保型脲醛树脂粘合剂合成的研究 总被引:1,自引:0,他引:1
脲醛树脂是广泛用于木材工业中一种重要的胶粘剂。对降低脲醛树脂粘合剂中游离甲醛含量的措施以及改性方法进行了探讨,并在大量的实验基础上摸索出了一套简单实用的合成工艺,根据此合成方法生产的产品具有粘度好、反应条件易控制、甲醛含量低和成本低等优点,树脂的甲醛含量达到国家室内板用粘合剂游离甲醛含量标准(GB18 587-2001)。 相似文献
37.
芳烃化合物对普通小球藻毒性的QSAR研究 总被引:7,自引:0,他引:7
用DFT-B3LYP方法,在较高基组6-311G**水平下,全优化计算了23种苯胺、苯酚和苯甲醛衍生物。从中获得前线分子轨道能级(EHOMO,ELUMO),最正氢原子电荷Q ,最负非氢原子电荷Q-,及分子偶极矩和体积(μ,V)等描述符。结合文献中标题化合物对普通小球藻的毒性值(-logEC50)和疏水性参数(logKow),由线性回归方法成功建立QSAR模型。将-logEC50与logKow直接相关,发现标题物对普通小球藻的毒性均高于基本毒性,而多元线性回归所得二变量模型(-logEC50=-1.945 0.612logKow-7.690ELUMO,n=23,R2=0.834,F=50.322,SE=0.274,P=0.000,VIF=1.093)能更好地描述标题物对普通小球藻的毒性,由此推断,标题物取非共价作用毒性机理,其对普通小球藻的毒性作用分为两步,首先穿过细胞壁在细胞内富集,以logKow描述;其次与亲核试剂发生亲电反应,以ELUMO表示。 相似文献
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40.
采用了电缆材料绿色化的两大指标———毒性和有害元素含量的两种检测方法,即细胞毒性试验和电感耦合等离子体发射光谱仪法,认为这两种方法具有简便、精确、快速和省钱等优点,文中也提供了某电缆阻水膏和某种电缆护套料分别进行毒性试验和有害元素检测和分析。 相似文献