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Paul F. Basch 《Journal of chemical ecology》1986,12(8):1679-1686
An understanding of reproductive function is important for control of parasitic helminths. In cestodes and trematodes virtually nothing is known about regulatory and coordinating mechanisms that control maturation, gamete formation, egg production, and related processes. Neurosecretory neurons have been reported in various species but specific modes of action of neurohormones have yet to be demonstrated. The role of ecdysone is being investigated. 相似文献
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Simultaneous fixation was investigated for a marine organism: the monogenean flatworm ectoparasite Merizocotyle icopae. Four protocols for primary fixation were compared: 3% glutaraldehyde alone in 0.1M cacodylate buffer for a minimum of 2 hours; 1% glutaraldehyde in combination with 1% osmium tetroxide, both in 0.1M cacodylate buffer, until tissues darkened (5-20 minutes); 1% glutaraldehyde in 0.1M cacodylate buffer in combination with 0.5% potassium ferricyanide-reduced osmium until tissues darkened (5-20 minutes); 1% glutaraldehyde in combination with 1% osmium tetroxide, both in 0.1M cacodylate buffer, for 30 minutes. The study confirms that the standard method for transmission electron microscopic fixation (first listed protocol) routinely applied to platyhelminths is optimal for ultrastructural preservation, but some simultaneous fixation methods (second and third listed protocols) are acceptable when rapid immobilization is needed. Scanning electron microscopic preparations may be improved using simultaneous primary fixation. 相似文献
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