企业文化建设与实践研究——以湖北白莲河抽水蓄能有限公司为例

在白莲河抽水蓄能电站建设中,重视企业文化建设来提高员工综合素质和凝聚力,促使企业各种要素和谐发展,取得了高的效益。     

Highly Organized Porous Gelatin-Based Scaffold by Microfluidic 3D-Foaming Technology and Dynamic Culture for Cartilage Tissue Engineering

A gelatin-based hydrogel scaffold with highly uniform pore size and biocompatibility was fabricated for cartilage tissue engineering using microfluidic 3D-foaming technology. Mainly, bubbles with different diameters, such as 100 μm and 160 μm, were produced by introducing an optimized nitrogen gas and gelatin solution at an optimized flow rate, and N₂/gelatin bubbles were formed. Furthermore, a cross-linking agent (1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide, EDC) was employed for the cross-linking reaction of the gelatin-based hydrogel scaffold with uniform bubbles, and then the interface between the close cells were broken by degassing. The pore uniformity of the gelatin-based hydrogel scaffolds was confirmed by use of a bright field microscope, conjugate focus microscope and scanning electron microscope. The in vitro degradation rate, mechanical properties, and swelling rate of gelatin-based hydrogel scaffolds with highly uniform pore size were studied. Rabbit knee cartilage was cultured, and its extracellular matrix content was analyzed. Histological analysis and immunofluorescence staining were employed to confirm the activity of the rabbit knee chondrocytes. The chondrocytes were seeded into the resulting 3D porous gelatin-based hydrogel scaffolds. The growth conditions of the chondrocyte culture on the resulting 3D porous gelatin-based hydrogel scaffolds were evaluated by MTT analysis, live/dead cell activity analysis, and extracellular matrix content analysis. Additionally, a dynamic culture of cartilage tissue was performed, and the expression of cartilage-specific proteins within the culture time was studied by immunofluorescence staining analysis. The gelatin-based hydrogel scaffold encouraged chondrocyte proliferation, promoting the expression of collagen type II, aggrecan, and sox9 while retaining the structural stability and durability of the cartilage after dynamic compression and promoting cartilage repair.     

新沂文化艺术中心设计

通过介绍新沂市文化活动中心的设计方案,论述从重视区域性文化的历史传承开始,重新认识潜藏于深层结构中的中国智慧与思维范式,去寻求中国建筑文化新的精神象征。     

中英商标的文化特色与翻译

从保留文化特色的思路出发,从翻译技巧的角度,对如何处理商标翻译中的民族文化特色进行了探讨。     

苗族文化在鞋靴设计中的美学表现和寓意传递

为了让鞋靴设计富有中国传统文化气息,将苗族文化与鞋靴设计相结合.苗族文化是中国文化的一个重要组成部分,具有鲜明的文化特点,以苗族文化在鞋靴设计中的应用为研究内容,阐述了苗族文化的传统工艺、苗族图腾及纹样种类,将苗族工艺、图腾纹样以及苗族色彩具体应用于鞋靴设计,实现苗族文化与鞋靴设计的完美融合,从而为现代化鞋靴赋予新的文...     

新时代背景下劳模文化引导高校开展劳动教育的 实践路径探析

劳模文化是以劳模为代表的先进劳动者的精神风貌和工作方式的集中体现, 作为中华民族精神和时代精神的重要组成部分, 体现了爱国主义和改革创新的核心价值。加强大学生的劳动教育是高校贯彻落实立德树人根本任务之所需, 更是推动大学生全面发展之所应, 而劳模文化为高校劳动教育的开展提供了鲜活案例和丰富教学资源。将探索新时代劳模文化引导高校开展劳动教育的实践路径, 以此树立大学生正确的劳动观, 助力培育具有高尚品德和职业素养的时代新人。     

Morphological and Biological Evaluations of Human Periodontal Ligament Fibroblasts in Contact with Different Bovine Bone Grafts Treated with Low-Temperature Deproteinisation Protocol

Several types of deproteinised bovine bone mineral (DBBM) are available on the market, and each one is obtained with a thermic and chemical process that can differ, achieving different results. Currently, several protocols using low temperature are suggested to reduce the possible particle crystallisation during the production process. This study aimed to evaluate the biomorphological reaction of periodontal fibroblast cultures in contact with different DBBM particles treated with a low-temperature protocol (Thermagen^®) and without exposure to sodium hydroxide (NaOH). Morphological evaluation was performed using light, confocal laser, and scanning electron microscopy, and the biological reaction in terms of proliferation was performed using an XTT proliferation assay at 24 h (T1), 72 h (T2), and 7 days (T3). The morphological analysis highlighted how the presence of the materials stimulated a change in the morphology of the cells into a polygonal shape, surface reactions with the thickening of the membrane, and expression of actin. In particular, the morphological changes were appreciable from T1, with a progressive increase in the considered morphological characteristics at T2 and T3 follow-ups. The proliferation assay showed a statistical significance between the different experimental materials and the negative control in T2 and T3 follow-ups. The post hoc analysis did not reveal any differences between the materials. In conclusion, the grafts obtained with the low-temperature extractions protocol and not exposed to NaOH solution showed positive morphological reactions with no differences in the sizes of particles.     

Sulfated Hyaluronan Binds to Heparanase and Blocks Its Enzymatic and Cellular Actions in Carcinoma Cells

We examined whether sulfated hyaluronan exerts inhibitory effects on enzymatic and biological actions of heparanase, a sole endo-beta-glucuronidase implicated in cancer malignancy and inflammation. Degradation of heparan sulfate by human and mouse heparanase was inhibited by sulfated hyaluronan. In particular, high-sulfated hyaluronan modified with approximately 2.5 sulfate groups per disaccharide unit effectively inhibited the enzymatic activity at a lower concentration than heparin. Human and mouse heparanase bound to immobilized sulfated hyaluronan. Invasion of heparanase-positive colon-26 cells and 4T1 cells under 3D culture conditions was significantly suppressed in the presence of high-sulfated hyaluronan. Heparanase-induced release of CCL2 from colon-26 cells was suppressed in the presence of sulfated hyaluronan via blocking of cell surface binding and subsequent intracellular NF-κB-dependent signaling. The inhibitory effect of sulfated hyaluronan is likely due to competitive binding to the heparanase molecule, which antagonizes the heparanase-substrate interaction. Fragment molecular orbital calculation revealed a strong binding of sulfated hyaluronan tetrasaccharide to the heparanase molecule based on electrostatic interactions, particularly characterized by interactions of (−1)- and (−2)-positioned sulfated sugar residues with basic amino acid residues composing the heparin-binding domain-1 of heparanase. These results propose a relevance for sulfated hyaluronan in the blocking of heparanase-mediated enzymatic and cellular actions.     

男同性恋群体的城市空间分布及文化生产:以沈阳为例

From the pespective of space-culture, this paper explores how gay people's body, behavior and social relations are organized, represented and understood in Shenyang city by adopting participating observation, in-depth individual interview and historical literature review. Through the interaction between the urban space and the gay groups' inner characteristics, this paper finds out that the urban space constructs a dynamic platform for the mobilization of homosexual behavior, emotion and identity.     

THE RELATIONSHIP BETWEEN DIAL SIZE,READING DISTANCE AND READING ACCURACY

The experiment reported suggests that the accuracy of reading a graduated scale is related to the angle subtended at the eye by the called space. There is a critical point representing about 98 per cent accuracy (for all intervals interpolated into five or less parts) below which there is a rapid fall off in accuracy and above which little improvement can be expected. Each combination of scale interval and called interval has a different critical point and from these it is possible to calculate the optimum reading distance for any dial size and vice versa. It is possible also to decide which of several alternative graduation systems is likely to be the best.

It was found that ‘ practised subjects ’—who had made more than 2000 readings on a particular graduation system—were able to read a dial at double the distance of an ‘ unpractised subject ’ both having 6/6 binocular eyesight. For ‘ practised subjects ’ with 6/12 binocular eyesight the reading distances were reduced by one fifth.