全文获取类型
收费全文 | 39篇 |
免费 | 3篇 |
专业分类
综合类 | 1篇 |
化学工业 | 16篇 |
机械仪表 | 4篇 |
建筑科学 | 2篇 |
轻工业 | 2篇 |
石油天然气 | 2篇 |
无线电 | 6篇 |
一般工业技术 | 2篇 |
原子能技术 | 1篇 |
自动化技术 | 6篇 |
出版年
2024年 | 1篇 |
2022年 | 1篇 |
2021年 | 1篇 |
2017年 | 3篇 |
2014年 | 2篇 |
2013年 | 1篇 |
2011年 | 4篇 |
2010年 | 2篇 |
2009年 | 1篇 |
2008年 | 6篇 |
2007年 | 1篇 |
2006年 | 2篇 |
2005年 | 2篇 |
2004年 | 3篇 |
2003年 | 2篇 |
2001年 | 2篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1985年 | 1篇 |
排序方式: 共有42条查询结果,搜索用时 15 毫秒
11.
Christina Polasky Armin Steffen Kristin Loyal Christian Lange Karl-Ludwig Bruchhage Ralph Pries 《International journal of molecular sciences》2021,22(21)
Obstructive sleep apnea (OSA) is characterized by nocturnal breathing intermissions resulting in oxidative stress and eventually, a low-grade systemic inflammation. The study aimed to investigate the impact of positive airway pressure (PAP) therapy on the inflammatory milieu as measured by monocyte and T cell phenotypic alterations. Participants were assessed for their OSA severity before PAP therapy and about six months later, including patient-reported outcome and therapy usage by telemetry readout. The distributions of the CD14/CD16-characterized monocyte subsets as well as the CD4/CD8-characterized effector T cell subsets with regard to their PD-1 and PD-L1 expression were analyzed by flow cytometry from blood samples. Data of 25 patients revealed a significant reconstitution of the monocyte subset distribution and a decrease in PD-L1 expression on pan-monocytes and CD8+ T cells without an association to initial AHI and overweight. The PD-1 expression was still increased on T cell subsets, especially on CD4+ TH17/22 cells. We conclude that PAP therapy might have a rapid effect on the monocyte phenotype and overall PD-L1 expression levels. However, T cell immune alterations especially on TH17/22 cells persist longer, indicating an ongoing disturbance of the adaptive immune system. 相似文献
12.
13.
《Food Control》2017
In 2013, the European Union (EU) allowed the inclusion of non-ruminant processed animal proteins (PAP) in fish feeds. Plans also exist to allow non-ruminant PAP in poultry and pig feeds but to keep intraspecies recycling forbidden (e.g. poultry to poultry or pig to pig). However, due to the fear of transmissible spongiform encephalopathy (TSE), PAP from ruminant species remains illegal to use in feeds. To ensure feed safety, nations across Europe need to safeguard that commercial fish feeds do not contain PAP from ruminant species. In this work we report a multi-laboratory study on the detection of ruminant PAP in commercial PAP feed ingredients. A total of 19 non-ruminant PAP, provided by the European Fat Processors and Renderers Association (EFPRA), were analyzed for ruminant content with the European Union Reference Laboratory (EURL)-validated PCR assay by three labs and compared to the result obtained with an immunoassay-based method (MELISA-TEK). Ruminant PAP was detected in five of the 19 non-ruminant PAP samples with both methods, but there were methodological and multi-laboratory differences for the PCR method. Compared to the results with the EU-reference laboratory, 3 false-negatives were found by the two other laboratories. We speculate that the discrepancies seen for some of the samples determined with the PCR method rely on the homogenization and grinding step, which is not described in detail in the EURL Standard Operating Procedure (SOP), shift in the normal distribution of Ct-values near the cut-off of the PCR method, or PCR inhibition. In conclusion, this study demonstrates the presence of ruminant DNA in commercially available non-ruminant PAP by use of two methods for the detection of PAP in commercial feed ingredients, and highlights the importance of thorough homogenization prior to DNA extraction and assessment of normal distribution of Ct-values for successful PCR detection at low ruminant DNA contamination levels. 相似文献
14.
从多年来室内燃气管道设计和施工所遇到的问题着手,结合工作实践和理论研究,分析探讨了几种解决问题的方法。 相似文献
15.
介绍了聚乙烯(PE)管、交联聚乙烯(PEX)管、耐高温非交联PE管、超高摩尔质量PE管、铝塑复合管5种聚乙烯系新型塑料给水管的性能特点及其发展。 相似文献
16.
对氨基苯酚的技术、应用及市场 总被引:2,自引:0,他引:2
分析了对氨基苯酚的国内外生产及消费概况,评述了对 氨基苯酚的各种工艺技术路线;对最有发展前途的两种先进工艺(硝基苯电解还原和硝基苯催化加氢还原)给予了重点介绍。 相似文献
17.
18.
Janice M. Sowinski Robert L. Mackay Santo V. Nicosia 《Microscopy research and technique》1985,2(4):293-303
The presence of nonspecific staining artifacts is a potential problem in ultrastructural immunocytochemistry. In the course of staining for lysozyme in the human and rabbit endocervix, the peroxidase-antiperoxidase complex (PAP) was found to bind to mucous granules in a selective and nonspecific manner. Sections etched in 10% aqueous H2O2, incubated for 5 minutes in 1:50 PAP in Tris-buffered saline (TBS) and subsequently treated with 3, 3′-diaminobenzidine-H2O2, revealed a staining precipitate within the matrix of mucous granules. The same selective and nonspecific staining could also be visualized when horseradish peroxidase (1 mg/ml) was substituted for PAP in the immunocytochemical sequence. Thus, this staining method made the reliable demonstration of the desired antigen difficult. The affinity of PAP for mucous granules can be completely eliminated by subjecting sections, previously etched in H2O2 to expose the antigenic sites, to a 3-minute incubation in immunoglobulin (1:10 human IgA, 1:5 human IgG, or 1:5 antihuman IgG) immediately prior to using PAP in the standard immunocytochemical staining sequence. The use of this modification to Sternberger's unlabeled antibody-enzyme method is recommended because it allows for the elimination of all nonspecific staining artifacts without interfering with specific localization by the primary antibody. The mechanism that causes nonspecific binding of peroxidase to mucous granule constituents is unclear, although carbohydrate binding may play a role in the interaction between mucous granules and peroxidase. 相似文献
19.
本文系统地观察了自然感染性鼠痘与实验性鼠痘的临床表现与病理形态学特征;讨论了鼠痘病理形态诊断的依据以及鼠痘与小鼠其它常见传染病的鉴别诊断要点;并采用免疫酶组织化学PAP技术,检测了鼠痘存档石蜡块中鼠痘病毒抗原在各脏器中的定位形态特点与分布规律。其目的在于将病理组织学诊断与特异性病原诊断,在形态学上结合起来,以达到早期、快速、准确的诊断目的。 相似文献
20.
重点介绍了建筑给水塑料管中占主导地位的铝塑复合管,无规共聚聚丙烯鉴定的生产工艺、产品特性和应用情况,通过市场分析,提出了给水塑料管的发展优势,指出其具有巨大的市场潜力。 相似文献