血清PSA及PAP测定的临床应用评价

报道了２３例前列腺癌、４２例良性前列腺增生及２１例男性非前列腺疾病住院患者血清ＰＳＡ及ＰＡＰ联合测定结果。前列腺癌组与良性前列腺增生及对照组比较有极显著差异，按决策矩阵法对血清ＰＳＡ及ＰＡＰ进行临床综合评价，两者诊断指数和可用度分别为１３５．８％、１３８。３％和０．３６、０．４５。结果表明血清ＰＳＡ及ＰＡＰ测定对于前列腺癌诊断及鉴别诊断为互补关系，血清ＰＳＡ测定作为前列腺癌治疗效果监测指标优于血清     

嵌入式Linux路由器中同步PPP的实现方案

分析了在做入式Linux路由器上实现同步PPP的问题并设计了几种解决方案。通过对各种方案的对比确定了一种最优方案,并将其实现。实验结果表明,该方案可以有效地进行同步PPP的PAP和CHAP认证以及NCP协商,并能够达到同步串口速率为2Mbps。     

碱改性气化煤泥对水溶液中对氨基苯酚的吸附

采用NaOH溶液对气化煤泥（CS-R）进行改性,考察了改性时间、温度、NaOH溶液浓度、液固比等因素的影响,利用静态吸附法研究了碱改性煤泥（CS-OH）对水溶液中对氨基苯酚（PAP）的吸附性能.结果表明,碱改性的最佳工艺条件是：改性时间24h,温度100℃,液固比10∶1,NaoH溶液浓度2 mol/L;CS-OH吸附PAP的综合性能优于CS-R.对于低浓度PAP废水,经CS-OH处理后可以达到国家工业废水排放标准.     

对氨基苯酚的技术、应用及市场

分析了对氨基苯酚的国内外生产及消费概况,评述了对 氨基苯酚的各种工艺技术路线;对最有发展前途的两种先进工艺（硝基苯电解还原和硝基苯催化加氢还原）给予了重点介绍。     

聚乙烯系新型塑料给水管的发展

介绍了聚乙烯（PE）管、交联聚乙烯（PEX）管、耐高温非交联PE管、超高摩尔质量PE管、铝塑复合管5种聚乙烯系新型塑料给水管的性能特点及其发展。     

室内燃气管道设计和施工有关问题的分析

从多年来室内燃气管道设计和施工所遇到的问题着手，结合工作实践和理论研究，分析探讨了几种解决问题的方法。     

Multi-laboratory evaluation of a PCR method for detection of ruminant DNA in commercial processed animal proteins

In 2013, the European Union (EU) allowed the inclusion of non-ruminant processed animal proteins (PAP) in fish feeds. Plans also exist to allow non-ruminant PAP in poultry and pig feeds but to keep intraspecies recycling forbidden (e.g. poultry to poultry or pig to pig). However, due to the fear of transmissible spongiform encephalopathy (TSE), PAP from ruminant species remains illegal to use in feeds. To ensure feed safety, nations across Europe need to safeguard that commercial fish feeds do not contain PAP from ruminant species. In this work we report a multi-laboratory study on the detection of ruminant PAP in commercial PAP feed ingredients. A total of 19 non-ruminant PAP, provided by the European Fat Processors and Renderers Association (EFPRA), were analyzed for ruminant content with the European Union Reference Laboratory (EURL)-validated PCR assay by three labs and compared to the result obtained with an immunoassay-based method (MELISA-TEK). Ruminant PAP was detected in five of the 19 non-ruminant PAP samples with both methods, but there were methodological and multi-laboratory differences for the PCR method. Compared to the results with the EU-reference laboratory, 3 false-negatives were found by the two other laboratories. We speculate that the discrepancies seen for some of the samples determined with the PCR method rely on the homogenization and grinding step, which is not described in detail in the EURL Standard Operating Procedure (SOP), shift in the normal distribution of Ct-values near the cut-off of the PCR method, or PCR inhibition. In conclusion, this study demonstrates the presence of ruminant DNA in commercially available non-ruminant PAP by use of two methods for the detection of PAP in commercial feed ingredients, and highlights the importance of thorough homogenization prior to DNA extraction and assessment of normal distribution of Ct-values for successful PCR detection at low ruminant DNA contamination levels.     

A method for abolishing the nonspecific binding artifacts produced by the peroxidase-antiperoxidase complex (PAP) in immunostained mucous epithelia

The presence of nonspecific staining artifacts is a potential problem in ultrastructural immunocytochemistry. In the course of staining for lysozyme in the human and rabbit endocervix, the peroxidase-antiperoxidase complex (PAP) was found to bind to mucous granules in a selective and nonspecific manner. Sections etched in 10% aqueous H₂O₂, incubated for 5 minutes in 1:50 PAP in Tris-buffered saline (TBS) and subsequently treated with 3, 3′-diaminobenzidine-H₂O₂, revealed a staining precipitate within the matrix of mucous granules. The same selective and nonspecific staining could also be visualized when horseradish peroxidase (1 mg/ml) was substituted for PAP in the immunocytochemical sequence. Thus, this staining method made the reliable demonstration of the desired antigen difficult. The affinity of PAP for mucous granules can be completely eliminated by subjecting sections, previously etched in H₂O₂ to expose the antigenic sites, to a 3-minute incubation in immunoglobulin (1:10 human IgA, 1:5 human IgG, or 1:5 antihuman IgG) immediately prior to using PAP in the standard immunocytochemical staining sequence. The use of this modification to Sternberger's unlabeled antibody-enzyme method is recommended because it allows for the elimination of all nonspecific staining artifacts without interfering with specific localization by the primary antibody. The mechanism that causes nonspecific binding of peroxidase to mucous granule constituents is unclear, although carbohydrate binding may play a role in the interaction between mucous granules and peroxidase.     

鼠痘的病理诊断及鼠痘病毒抗原定位的研究

本文系统地观察了自然感染性鼠痘与实验性鼠痘的临床表现与病理形态学特征;讨论了鼠痘病理形态诊断的依据以及鼠痘与小鼠其它常见传染病的鉴别诊断要点;并采用免疫酶组织化学PAP技术,检测了鼠痘存档石蜡块中鼠痘病毒抗原在各脏器中的定位形态特点与分布规律。其目的在于将病理组织学诊断与特异性病原诊断,在形态学上结合起来,以达到早期、快速、准确的诊断目的。     

建筑给水塑料管发展前景

重点介绍了建筑给水塑料管中占主导地位的铝塑复合管，无规共聚聚丙烯鉴定的生产工艺、产品特性和应用情况，通过市场分析，提出了给水塑料管的发展优势，指出其具有巨大的市场潜力。