Distinct Expression Patterns of Cxcl12 in Mesenchymal Stem Cell Niches of Intact and Injured Rodent Teeth

Specific stem cell populations within dental mesenchymal tissues guarantee tooth homeostasis and regeneration throughout life. The decision between renewal and differentiation of stem cells is greatly influenced by interactions with stromal cells and extracellular matrix molecules that form the tissue specific stem cell niches. The Cxcl12 chemokine is a general marker of stromal cells and plays fundamental roles in the maintenance, mobilization and migration of stem cells. The aim of this study was to exploit Cxcl12-GFP transgenic mice to study the expression patterns of Cxcl12 in putative dental niches of intact and injured teeth. We showed that endothelial and stromal cells expressed Cxcl12 in the dental pulp tissue of both intact molars and incisors. Isolated non-endothelial Cxcl12⁺ dental pulp cells cultured in different conditions in vitro exhibited expression of both adipogenic and osteogenic markers, thus suggesting that these cells possess multipotent fates. Taken together, our results show that Cxcl12 is widely expressed in intact and injured teeth and highlight its importance as a key component of the various dental mesenchymal stem cell niches.     

“三全育人” 格局下高校辅导员队伍角色定位与转型论析

高校围绕立德树人根本使命,正在积极构建全员全过程全方位育人格局。在此形势下,作为高校思政工作一线骨干力量的辅导员队伍面临新的角色转型,找准自己在高校"大思政"中的新定位,要成为全员育人的骨干力量,做思想政治教育和管理工作的组织者与育人资源的协调者;要成为全过程育人的融通力量,做学生全面成长的指导者与持续发展的推动者;要成为全方位育人的串联力量,做"十大"育人体系落地的终端实施者与成效评估者。在三全育人的新格局下,辅导员队伍要与校内外育人队伍一起,协同攻关、形成合力,共同推进一体化育人机制。     

冠脉造影图像血管增强及减影的优化方法

针对于传统X射线冠状动脉造影序列图像的血管的减影包含严重伪影以及减影结果不完整等问题,提出了冠脉造影图像血管增强以及减影优化的方法。基于冠脉造影序列图像的时空特性,将相同时相的图像匹配进行减影,得到初步减影结果。针对初步减影部分缺失,对有导管遮挡没有被识别的单根血管进行二次增强处理。增强方法为:运用区域生长确定被导管遮挡的血管部分,以此部分为基础按规定模板扩展,形成覆盖导管区域,区分血管和导管,改变两部分的灰度值,增大对比度。最后采用灰度线性变换、中值滤波等步骤,得到优化的冠脉血管减影。实验表明,本减影及优化方法可以从冠脉造影序列图中提取较为完整清晰的冠脉血管并且具有很好的可控性和鲁棒性。     

Exploring the Biomaterial-Induced Secretome: Physical Bone Substitute Characteristics Influence the Cytokine Expression of Macrophages

In addition to their chemical composition various physical properties of synthetic bone substitute materials have been shown to influence their regenerative potential and to influence the expression of cytokines produced by monocytes, the key cell-type responsible for tissue reaction to biomaterials in vivo. In the present study both the regenerative potential and the inflammatory response to five bone substitute materials all based on β-tricalcium phosphate (β-TCP), but which differed in their physical characteristics (i.e., granule size, granule shape and porosity) were analyzed for their effects on monocyte cytokine expression. To determine the effects of the physical characteristics of the different materials, the proliferation of primary human osteoblasts growing on the materials was analyzed. To determine the immunogenic effects of the different materials on human peripheral blood monocytes, cells cultured on the materials were evaluated for the expression of 14 pro- and anti-inflammatory cytokines, i.e., IL-6, IL-10, IL-1β, VEGF, RANTES, IL-12p40, I-CAM, IL-4, V-CAM, TNF-α, GM-CSF, MIP-1α, Il-8 and MCP-1 using a Bio-Plex^® Multiplex System. The granular shape of bone substitutes showed a significant influence on the osteoblast proliferation. Moreover, smaller pore sizes, round granular shape and larger granule size increased the expression of GM-CSF, RANTES, IL-10 and IL-12 by monocytes, while polygonal shape and the larger pore sizes increased the expression of V-CAM. The physical characteristics of a bone biomaterial can influence the proliferation rate of osteoblasts and has an influence on the cytokine gene expression of monocytes in vitro. These results indicate that the physical structure of a biomaterial has a significant effect of how cells interact with the material. Thus, specific characteristics of a material may strongly affect the regenerative potential in vivo.     

Augment low-field intra-operative MRI with preoperative MRI using a hybrid non-rigid registration method

Background

Preoperatively acquired diffusion tensor image (DTI) and blood oxygen level dependent (BOLD) have been proved to be effective in providing more anatomical and functional information; however, the brain deformation induced by brain shift and tumor resection severely impairs the correspondence between the image space and the patient space in image-guided neurosurgery.

Method

To address the brain deformation, we developed a hybrid non-rigid registration method to register high-field preoperative MRI with low-field intra-operative MRI in order to recover the deformation induced by brain shift and tumor resection. The registered DTI and BOLD are fused with low-field intra-operative MRI for image-guided neurosurgery.

Results

The proposed hybrid registration method was evaluated by comparing the landmarks predicted by the hybrid registration method with the landmarks identified in the low-field intra-operative MRI for 10 patients. The prediction error of the hybrid method is 1.92 ± 0.54 mm, and the compensation accuracy is 74.3 ± 5.0%. Compared to the landmarks far from the resection region, those near the resection region demonstrated a higher compensation accuracy (P-value = .003) although these landmarks had larger initial displacements.

Conclusions

The proposed hybrid registration method is able to bring preoperatively acquired BOLD and DTI into the operating room and compensate for the deformation to augment low-field intra-operative MRI with rich anatomical and functional information.     

红细胞瞬间磁化试剂的制备与应用

目的研制用于ABO、RHD血型检测试剂的红细胞瞬间磁化试剂。方法采用共沉淀法制备磁性粒子,并检测其主要成分及粒径大小,用此磁性粒子制备红细胞瞬间磁化试剂,并分别进行各项检测。结果磁性粒子的主要成分为四氧化三铁,粒径100nm左右。该试剂的外观及特异性均合格,磁响应时间不超过30s,重复性较好,敏感性为98.20%,与试管法检测结果比较,差异无显著意义。结论红细胞瞬间磁化试剂可用于红细胞系统的血型检测。     

水稻全生育期应用腐植酸液肥的效果初报

腐植酸液肥——科力素(KLS)对水稻生长发育影响较大。试验表明,水稻苗床施用KLS明显刺激秧苗生长,处理区根长、根数、地上地下百株鲜重及带蘖率都好于对照区。不同生育时期喷施KLS,水稻发育加快,自返青期开始到成熟期都有不同程度提前,一般早熟3～5d。产量增加幅度为4.7%～21.1%,平均增产11.3%,亩增效37.44～204.48元,平均为99.2元,产投比约为12∶1。     

户内变电站三维设计应用研究

目前,户内变电站普遍采用二维设计,整个工程设计依靠设计人员的空间想象力和工程制图技能完成三维空间设计,这种方法缺乏对设计质量的有效控制,已难以适应目前各电网公司对设计精细化的需求。户内变电站三维设计具有直观、智能等特点,在适应了复杂的户内变电站设计、不断压缩的设计周期和设计资料的数字化移交等要求的同时,完善了工程设计人员充分表达设计思想的手段,是一种能提高项目质量的助推器,也是实现变电工程全寿命周期管理的有效工具,更是未来变电工程设计的发展趋势。     

烟丝整丝率变化率对卷烟卷接质量控制的影响研究

长期以来,烟丝的物理特征都被认为是影响卷烟质量的主要因素。随着生产工艺和企业烟丝标准的不断提高,如今烟丝的物理特征对卷烟质量的影响不再显著。研究人员转而将目光转向了烟丝的整丝率变化率。