乙硫醇与金表面相互作用的TOF—SIMS研究

利用飞行时间型静态二次离子质谱（TOF－SIMS）,以乙硫醇为代表,在二次离子质谱的特定条件下,分析了乙硫醇与金的表面相互作用,发现了丰富的、有一定规律性的金硫团簇。从而首次在质谱中再现了硫醇与金的分子自组装过程。这一结果不仅表明高性能静态二次离子质谱能够用于分子自组装;而且,有可能用于新类型自组装分子的探索。     

采用三次通信的TOF与TDOA联合定位算法

通常基于超宽带(UWB)的定位系统采用的主要技术是TOA和TDOA算法,但是TOA算法定位速度低,TDOA算法定位精度不稳定,这些情况均限制了它们在UWB定位系统中的应用。为了权衡这两种算法在定位速度和精度方面的不足,提出了一种新的TOF与TDOA联合定位算法(CTT)。该算法仅需要3次UWB通信便可测量TDOA算法所需的所有时间差信息和一个TOF距离值,并可以此计算出该定位区域内所有基站与单个标签的距离,定位速度比TOA算法提升至少50%。使用DW1000分别对TOA、TDOA与CTT算法进行定位精度对比,结果表明CTT算法在二维情况下的平均定位误差<20 cm,标准差<10 cm,接近于TOA的精度并解决了TDOA算法精度不稳定的问题。     

Thymosin β4 is differentially expressed in the cerebrospinal fluid of Creutzfeldt‐Jakob disease patients: a MALDI‐TOF MS protein profiling study

MALDI‐TOF protein profiling analysis permits the detection of peptides and small proteins in complex protein mixtures with great accuracy. We applied this analysis to cerebrospinal fluid (CSF) from 15 patients affected by Creutzfeldt‐Jakob disease (CJD). We compared the levels of the normalized ion signals of 11 sporadic and 4 genetic CJD forms with those from ten healthy control subjects and eight non‐CJD relapsing‐remitting multiple sclerosis patients. In so doing, we detected 61 differentially expressed ion signals in CJD samples compared to controls. Among the 61 signals, 3 signals had significantly increased levels with high statistical significance (p <0.0001) and were located at 3238.3 m/z, 4963.7 m/z, and 8565.3 m/z. We characterized the 5.0 and 8.6 kDa proteins as thymosin β4 N‐acetylated and free ubiquitin, respectively, while the 3.2‐kDa peptide remained uncharacterized. Although elevated ubiquitin levels have previously been described in CJD, we have demonstrated for the first time the involvement of thymosin β4 in a neurodegenerative disease. To support the validity of thymosin β4 levels obtained by MALDI‐TOF analysis, an independent enzyme immunoassay analysis was performed. Moreover, a validation cohort consisting of CSF from three CJD patients, five healthy subjects, and six non‐CJD relapsing‐remitting multiple sclerosis patients was analyzed in a similar way, yielding superimposable results. We propose that thymosin β4 is a potential new candidate marker for the ante mortem diagnosis of CJD disease.     

Detection of colorectal adenoma and cancer based on transthyretin and C3a-desArg serum levels

Colorectal cancer is the second leading cause of cancer death, and it develops from benign colorectal adenomas in over 95% of patients. Early detection of these cancer precursors by screening tests and their removal can potentially eradicate more than 95% of colorectal cancers before they develop. To discover sensitive and specific biomarkers for improvement of pre‐clinical diagnosis of colorectal adenoma and cancer, we analysed in two independent studies (n = 87 and n = 83 patients) serum samples from colorectal cancer (stage III), colorectal adenoma and control patients using SELDI‐TOF‐MS. Extensive statistical analysis was performed to establish homogeneous patient groups based on their clinical data. Two biomarkers that were each able to distinguish control patients from either colorectal adenoma or colorectal cancer patients (p<0.001) were identified as transthyretin (pre‐albumin) and C3a‐desArg by MS/MS and were further validated by antibody‐based assays (radial immunodiffusion, ELISA). A combination of both proteins clearly indicated the presence of colorectal adenoma or carcinoma. Using a cut‐off of <0.225 g/L for transthyretin and >1974 ng/mL for C3a‐desArg, we found a sensitivity and specificity for colorectal adenoma of 96% and 70%, respectively.     

Assessment by Matrix‐Assisted Laser Desorption/Ionization Time‐of‐Flight Mass Spectrometry of the Effects of Preanalytical Variables on Serum Peptidome Profiles Following Long‐Term Sample Storage

Purpose

Human serum and plasma are often used as clinical specimens in proteomics analyses, and peptidome profiling of human serum is a promising tool for identifying novel disease‐associated biomarkers. Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) is widely used for peptidomic biomarker discovery. Careful sample collection and handling are required as either can have a profound impact on serum peptidome patterns, yet the effects of preanalytical variables on serum peptidome profiles have not been completely elucidated. The present study investigated the effects of preanalytical variables, including storage temperature, duration (up to 12 months), and thawing methods, on MALDI‐TOF MS‐based serum peptidome patterns.

Experimental design

Aliquots of serum samples were pretreated with weak cation exchanger magnetic beads using an automated ClinProtRobot system and then analyzed by MALDI‐TOF MS.

Results

A number of significant differences in peak intensities were observed depending on sample processing variables.

Conclusions and clinical relevance

These peaks can be used as sample quality markers to assess the effects of long‐term storage on serum peptidome profiles using MALDI‐TOF MS.

     

基于计算机视觉的人体内腔三维重建技术综述

医生通过内窥镜观察的人体内腔显示为二维图像,不能立体地展现内腔环境中病灶、血管及邻近组织的关系,而内腔三维重建及可视化技术能够清晰、全面地展现病灶及其他组织的三维形态,更好地辅助医生进行精准的手术判断.将人体内腔环境中的三维重建技术分为主动式测量方法与被动式测量方法,分类综述基于结构光、飞行时间、双目立体视觉、单目视觉...     

Coarse head pose estimation of construction equipment operators to formulate dynamic blind spots

Several hundred workers die in construction in the United States every year because equipment operators are unable to see their fellow workers during operation of their vehicle. In this paper we propose a step towards improving this situation by providing an automated method based on range imaging for estimating the coarse head orientation of a construction equipment operator. This research utilizes commercially-available low resolution range cameras to measure the continuously changing field-of-view (FOV) of an equipment operator in outdoor construction. This paper presents a methodology to measure so-called dynamic blind spot maps. The dynamic blind spot map is then projected on a known static equipment blind spot map that already exists to each construction vehicle. A robust computational coarse head pose estimation algorithm and results to three different pieces of construction equipment and multiple operators are presented. The developed method has the potential in automatically determining the spaces around vehicles that are currently not in the field-of-view of the vehicle operator thus providing eventually additional means and technology for improving safety in construction.     

飞行时间三维相机的多视角散乱点云优化配准方法研究

针对目前基于飞行时间(TOF)原理的三维相机实现物体完整表面的三维点云重建过程中,多视角散乱点云配准精度低的问题,本文提出了一种多视角散乱点云优化配准方法。该方法通过构建一个目标功能函数,并结合相邻点云的变换矩阵对该目标函数进行最小化求解,直接获取任意位置的点云到基准坐标系的绝对变换矩阵,避免了对相邻点云的变换矩阵进行累积而引起误差的累加。实验结果表明,该方法提高了多视角点云配准的精度,同时增强了物体点云模型重建的效果,在三维曲面重建中具有较强的实用性。     

有限封闭环境中基于PCA的水声测距方法

有限空间中多途效应严重影响测距的准确性,针对该问题,提出一种基于主成分分析的水声信号测距方法,对信号协方差矩阵进行降维和特征抽取,在保留协方差矩阵信息量的同时抑制多途干扰信号的影响。实验结果表明,该方法能够提高有限封闭液态场环境中水声测距的准确性和稳健性。