XIV. Yeast sequencing reports. Sequence of MKT1, needed for propagation of M2 satellite dsRNA of the L-A virus of Saccharomyces cerevisiae

MKT1 is required for m aintenance of K₂ above 30°C in strains with the L-A-HN variant of the L-A double-stranded RNA virus of Saccharomyces cerevisiae. We report that MKT1 encodes a 92 979 Da protein with serine-rich regions and the retroviral protease signature, DTG, but with no substantial homology to proteins presently in the databases. This sequence is available from GenBank under Accession Number U09129.     

Yeast sequencing reports. Isolation and sequence analysis of a gene from the linear DNA plasmid pPAC1–2 of Pichia acaciae that shows similarity to a killer toxin gene of Kluyveromyces lactis

The toxin-encoding linear plasmid systems found in Pichia acaciae and Kluyveromyces lactis yeasts appear to be quite similar, both in function and structural organization. By Southern hybridization, a linear plasmid of P. acaciae, pPac1–2, was found to hybridize to the second open reading frame (ORF2) of K. lactis plasmid pGKL1, known to encode the α and β subunits of the K. lactis toxin. A 1·7 kbp segment of pPac1–2 DNA was cloned, sequenced and shown to contain four regions of strong homology to four similarly oriented regions of K. lactis ORF2. This 1·7 kbp fragment also contained an ORF of 1473 bp that could encode a protein of ～ 55·8 kDa. Like the α subunit gene of K. lactis ORF2, a very hydrophobic region occurs at the N-terminus, perhaps representing a signal sequence for transport out of the cell. Unlike K. lactis ORF2, however, the encoded polypeptide is much smaller and lacks a recognizable domain common to chitinases. The structure of a toxin that includes the translation product of this P. acaciae ORF would likely be quite different from that of the K. lactis toxin. Analysis of the upstream region of the P. acaciae ORF revealed an upstream conserved sequence identical to that found before ORFs 8 and 9 of pGKL2. A possible hairpin loop structure, as has been described for each of the four K. lactis pGKL1 ORFs, was found just upstream of the presumed start codon. The similarity of the promoter-like elements found in the linear plasmid genes of these diverse yeasts reinforces the idea of the existence of a unique, but highly conserved, expression system for these novel plasmids. The sequence has been deposited in the GenBank data library under Accession Number U02596.     

Pb（Sc1／2Ta1／2）O3铁电体的有序结构和介电性质）

通过铌铁矿预合成法制备Ｐｂ（Ｓｃ１／２Ｔａ１／２）Ｏ３（ＰＳＴ）陶瓷，经适当的热处理获得了钙钛矿化合物Ｂ位离子的高有序和高无序试样，ＰＳＴ陶瓷的介电性质研究结果表明，高有序试样（Ｓ＝０．７７）的居里温度为２９℃，并具有较低的介电常数表现出普通铁电体性质特征，而高元序试样（Ｓ＝０）具有很高的介电常数，并表现出明显的弥散性相转变和Ｔｍ的频率色散行为，即高介电常数的驰豫铁电体性质。文中还利用ＸＲＤ，ＳＥ     

随钻气侵检测仪在胜利油田的应用

简要介绍了随钻气侵检测仪的结构特点、工作原理、现场模拟试验及应用情况。中原油田全尺寸实验井和胜利油田６口井的现场试验及应用结果表明，该仪器能实现早期气侵检测，比常规方法检测到气侵的时间明显提前，对高压气井和复杂地层井控具有重要的现实意义。     

Ti-23Al-14Nb-3V合金氩弧焊接头的显微组织及其力学性能

研究了预热对Ｔｉ－２３Ａｌ－１４Ｎｂ－３Ｖ合金焊接性，特别是对消除氩弧焊冷裂纹的作用以及对接头显微组织和力学性能的影响。结果表明，焊前预热可有效降低该合金的冷裂倾向。经预热处理后，焊缝区的结晶层状线消失，热影响区的硬度峰得到缓和，整个焊缝的显微硬度分布趋于均匀，但焊缝区的枝晶发生粗化，热影响区有所扩大。焊件接头的拉伸试验表明，预热可使焊缝区的接头强度系数增加，但无论预热与否，接头强度均低于母材强度，且不显示宏观塑性。     

Electrostatic polymer processing of isotactic poly(4-methyl-1-pentene) fibrous membrane

Isotactic poly(4-methyl-1-pentene) (P4M1P) is a widely used polymer in industrial applications and specifically, in medical products. Producing micro- or nanofibers would expand the usefulness of P4M1P to a broad range of medical applications. The choice and quality of solvent for the solution used for electrospinning can have a dramatic effect on the spinnability of fibers and on their morphological appearance. In this study, four solvent systems: cyclohexane, cyclohexane/acetone mixture, cyclohexane/dimethyl formamide (DMF) mixture and cyclohexane/acetone/DMF mixture have been investigated. As demonstrated by FE-SEM, electrospun fibers with different morphologies including round, twisted with a roughened texture, curled and twisted-ribbon shapes were formed. The fiber shape and morphology depended strongly on the type and amount of non-solvent used.     

LXT384接口芯片的几种环回形式

LXT384是一个用于SONET/SDH设备的八进制T1/E1/J1线路接口单元芯片。文中简述了该芯片在实际应用中进行自检的若干种不同的环回形式(包括：模拟环回、数字环回、运程环回等)，直观地指出了各种环回形式的特点和区别。     

膨胀水泥浆体系在苏丹1/2/4区块水平井固井中的应用

随着苏丹1／2／4区块的深入开发，为了加快开发主力油层，提高原油产量，降低钻井成本，GNPOC在此区块布置水平井开发任务。所开发的区域主要分布于Heglig，Munga，Simbir，Bamboo区块，油层所在是Zaqar和Bentiue地层。此层位具有高渗透，地层温度高，易塌、气窜等复杂问题，尤其是Heglig和Munga地区井壁坍塌严重。为此，根据地质资料、井身结构和对水平井注水泥的技术要求，科学合理设计施工工艺，优选出适合此地区固井施工的冲洗液和隔离液，特别是经过努力实践，开发出了新型膨胀水泥浆体系，此体系具有高强度、低失水、无析水、防气窜之功效。结合使用哈里伯顿CPT—Y4大功率固井车，精确计量和自动密度控制，该体系水泥浆目前已在7口水平井施工中得以应用，均为优质井。现正在被大量使用，此体系的开发，解决了该固井技术难题，创造了中国固井在海外首次水平井作业一次成功的历史记录，具有较高的经济和社会效益。     

科钻一井扩孔器的研制及应用

科钻一井在取心钻进中为了尽量避免大段划眼(扩孔),在钻具组合中岩心筒的上、下部位各装一套扩孔器,为此,采用无压浸渍焊接工艺研制出φ157．3 mm碳化钨胎体式表镶φ3mm×5 mm人造聚晶金刚石扩孔器。科钻一井99只扩孔器的现场应用证明,该扩孔器使用寿命较长,满足了全井取心钻进的需要,是一种较好的取心钻具配件,也有助于提高和完善我国硬岩深井大直径取心钻进技术。     

新型热红外伪装涂料用填料中空微珠性能研究

分析了温度对涂层发射率的影响，并据此重点研究了一种新型热红外伪装涂料用填料——中空微珠——的常规使用性能、降温效果以及对涂层发射率的影响。研究认为，中空微殊用量小于20％、粒径为200目左右时常规使用性能最好。60目的中空微殊降温效果最好，而且当其用量小于20％时，中空微珠对涂层的发射率基本没有影响。