A comparison of biological activities of a new soya biopeptide studied in an in vitro skin equivalent model and human volunteers

During aging, the epidermis and dermis become thin and an efficient anti-aging product should be able to stimulate the metabolism of senescent fibroblast and keratinocytes, in order to increase the quantity of extra-cellular matrix components such as collagen and glycosaminoglycans. A study performed in parallel on an in vitro skin equivalent model, and in vivo, with human volunteers, demonstrated the efficacy of one specific soya biopeptide for anti-aging properties. Such a biopeptide induces a significant increase of glycosaminoglycans synthesis in vitro and in vivo after a one-month treatment. We also showed that this new cosmetic ingredient is able to stimulate favourably the collagen synthesis in vitro and in vivo. This study provided the proof for anti-aging properties of a new soya biopeptide and also validated the skin equivalent model developed for this experimentation as an alternative method to animal or human testing for some cosmetic efficacy evaluations.     

Evaulation of irritation potential of surfactant mixtures

Irritation potential of sodium laureth sulfate (SLES) alone, and in combination with lauryl glucoside (LG), polysorbate 20 (PS) and cocoamidopropyl betaine (CAPB) was tested in 13 human subjects. Four main and six sub-formulations were prepared and evaluated. Formulations were applied to the forearm as a 24 h close patch study. Irritation was scored by two different methods using an in vivo clinical protocol based on visual scoring and on the stratum corneum capacitance measurement. Irritation was found to be dose dependent. At 2 mg/patch level ten subjects did not show any skin reaction. At 20 mg/patch level eleven subjects showed a broad range of skin irritation. The highest irritation was observed with the formula that contained SLES, LG, and cocamide DEA together. Among the sub-formulations, cocamide DEA showed the highest irritation grade. A statistically significant correlation was observed between visual, clinical and corneometer scores. It was concluded that the irritation potential of surfactants was related to the total surfactant concentration, application mode, and the thermodynamic activity of molecules in the solution as well as the chemical structure of the surfactant molecules.     

转鼓鞣制兔皮新工艺

本文在剖析兔皮组织结构的基础上,解决了转鼓鞣制兔皮生产中的几个关键性问题,制定了转鼓鞣制兔皮新工艺。     

CoFe涂层对非晶薄带GMI效应的影响

上制备不同厚度的CoFe薄膜层,观察其巨磁阻抗(GMI)效应在120 kHz~3 MHz频率范围内随外加磁场的变化。实验结果显示,在Co基非晶薄带上涂覆CoFe薄膜,可提高薄带的GMI效应。研究发现,当趋肤效应显著时材料表面粗糙度对GMI效应有较大影响。通过在Co基非晶薄带表面镀膜的方式降低样品表面粗糙度,减小表面退磁场的影响,并闭合样品磁通回路,从而提高样品GMI效应。     

基于图像内容及底层特征的敏感图像检测方法

内容安全是信息安全领域中的一个新兴且重要的研究方向.敏感图像分析与监控是该方向的一个重要研究分支,近年来愈来愈受到关注.然而,由于其研究起步较晚且图像本身所具有的复杂多样性,相对来说,目前已有的研究成果还比较少且性能上也有待于进一步提升,为此,本文提出了一种新的敏感图像分析检测方法.该方法从基于图像内容分析和基于图像底层特征分析两个方面进行综合分析检测,在基于内容分析方面,通过分析图像是否含有大量的皮肤区域来初步检测敏感图像;在基于图像底层特征分析方面,通过颜色直方图矢量和颜色聚合矢量提取图像的颜色及空间信息,并在此基础上利用SVM进行分类,从而实现敏感图像的初步检测;最后,对上述两方面的初步检测结果进行综合判决,实现敏感图像的最终检测.仿真实验结果出示了该新方法的有效性.     

用于皮肤影像诊断的光学成像方法

皮肤影像学是利用现代成像技术手段对皮肤病进行无创、原位、动态、实时诊断的一门新兴技术学科。在过去十几年里作为医学影像学分支的皮肤影像学取得了长足的进步,包括皮肤镜等多种光学成像技术已经被广泛应用于临床疾病诊断。主要介绍了皮肤镜、皮肤共聚焦、多光子成像、光学相干层析成像以及光声成像等技术在皮肤影像学中的发展和应用。这5种技术能够实现原位、在体、实时的皮肤成像,可对可疑部位进行重复检查,并能不同程度地实现皮下组织的无损成像,为临床诊断提供了客观的评价依据。不断发展的皮肤影像学,与皮肤组织病理学相互促进、相互补充,势必将带动现代皮肤病学的飞跃发展。     

Flexible Nanosomes (SECosomes) Enable Efficient siRNA Delivery in Cultured Primary Skin Cells and in the Viable Epidermis of Ex Vivo Human Skin

The extent to which nanoscale‐engineered systems cross intact human skin and can exert pharmacological effects in viable epidermis is controversial. This research seeks to develop a new lipid‐based nanosome that enables the effective delivery of siRNA into human skin. The major finding is that an ultraflexible siRNA‐containing nanosome—prepared using DOTAP, cholesterol, sodium cholate, and 30% ethanol—penetrates into the epidermis of freshly excised intact human skin and is able to enter into the keratinocytes. The nanosomes, called surfactant‐ethanol‐cholesterol‐osomes (SECosomes), show excellent size, surface charge, morphology, deformability, transfection efficiency, stability, and skin penetration capacity after complexation with siRNA. Importantly, these nanosomes have ideal characteristics for siRNA encapsulation, in that the siRNA is stable for at least 4 weeks, they enable highly efficient transfection of in vitro cultured cells, and are shown to transport siRNA delivery through intact human skin where changes in the keratinocyte cell state are demonstrated. It is concluded that increasing flexibility in nanosomes greatly enhances their ability to cross the intact human epidermal membrane and to unload their payload into targeted epidermal cells.     

银屑病患者皮损中角蛋白10、14、17及丝聚蛋白表达与分布

目的：研究银屑病患者皮损中角蛋白10、14、17及丝聚蛋白的分布、表达与皮肤屏障功能受损的相关性。方法以20例银屑病患者皮损及10例正常人皮肤为研究对象,运用免疫组化及westerm blot测定两组角蛋白10、14、17及丝聚蛋白的分布与表达。结果：与正常人皮肤相比,银屑病患者的皮损组织中角蛋白10表达和丝聚蛋白表达下调;角蛋白14及17表达上调,但表达程度及部位不同,其差异均具有统计学意义。结论银屑病患者的皮肤屏障功能有受损,可能是银屑病持续发病机制之一。