Intracellular localization of the antitumour drug adriamycin in living cultured cells: A confocal microscopy study

The intracellular distribution of the anthracyclinic antibiotic adriamycin in living cultured cells has been investigated by confocal microscopy. In human melanoma cells (M14), adriamycin was localized inside the nuclei. When adriamycin-treated M14 cells were allowed to recover in drug-free medium, a complete efflux of the drug from the nucleus was revealed. In recovered cells, a weakly fluorescent signal was observed in the perinuclear region. When M14 cells were recovered in a medium containing colcemid, a microtubule depolymerizing agent, the drug transport from the nucleus to the cell periphery appeared to be inhibited, suggesting that the microtubule network is strongly involved in drug transport mechanisms. In multidrug-resistant (MDR) cells the intracellular location of adriamycin was shown to be noticeably different from that of the parental wild-type cells. In particular, in resistant human breast carcinoma cells (MCF-7), adriamycin appeared to be exclusively located within the cytoplasm whereas the nuclei were shown to be completely negative. When adriamycin treatment was performed in association with MDR revertants, such as Lonidamine (inhibitor of the energy metabolism) or verapamil (inhibitor of the P-glycoprotein efflux pump), a marked enhancement of the cytoplasmic signal was observed in resistant cells. Under these conditions, adriamycin appeared concentrated in the perinuclear region, but the nuclei were still negative. Confocal microscopy proved to be a very useful method for the study of the intracellular transport of fluorescent substances, such as anthracyclinic antibiotics, and for the investigation of the multidrug resistance phenomenon in tumour cells.     

粉喷桩处理软弱地基在祁临高速公路的应用

结合工程实例，介绍了粉喷桩的所属类型，粉喷桩对软弱地基加固的原因，所适应土质及加固特点，阐述了祁临十八合同段的粉喷桩施工过程及施工中应注意的若干问题，得出粉喷桩处理不良地基效果良好。     

微型无阀泵参数化结构设计及测试

 从理论计算和试验两方面研究了用于微流控化学分析芯片的微型无阀泵流动特性，初步建立了微型无阀泵流道结构的数学模型，重点讨论了结构参数的选取原则及相应的流动阻尼系数的计算方法． 通过试验验证了数值计算的可靠性，为微型无阀泵参数化设计及优化提供了理论依据及经验曲线．     

Incoloy 800H合金的应用和性能分析

通过对耐热耐蚀合金Incoloy 80 0H的性能分析 ,指出它与普通奥氏体不锈钢相比具有较优的抗氧化性能、耐蚀性和高温蠕变强度。针对催化裂化装置同轴式沉降再生器内部所用Incoloy 80 0H焊件易出现裂纹的问题进行分析 ,认为在施焊过程中由于合金凝固时有低熔点金属或化合物的液态膜残留在晶界区 ,该合金具有高的焊接热裂纹敏感性 ,若焊材选择不当 ,焊丝填充不充分、缺少保护气等将导致晶间裂纹产生。提出了该合金采用TIG焊接防止热裂纹产生的措施。     

局部设有钢筋砼电梯井墙的设计探讨

本文就局部设有钢筋砼电梯井的工程计算及理论分析，阐明分析了少墙的框剪结构的设计及注意问题。     

激光熔覆Ni基SiC合金涂层组织与性能的研究

利用5kWCO2连续波激光器在16Mn钢基材表面对含20%(体积比)SiC陶瓷粉末的镍基自熔性合金粉末进行激光熔覆得到Ni基SiC合金涂层(NiSiC)。研究了合金涂层的组织形貌及相结构,并用单纯的镍基合金涂层(Ni60)进行了显微硬度及滑动磨损性能的对比试验。结果表明,NiSiC合金涂层由γ枝晶及其间的共晶组织组成,主要组成相为γ-Ni,γ-(Ni,Fe)固溶体和(Cr,Fe)7C3,Cr23C6及(Cr,Si)3Ni3Si等化合物。添加SiC的镍基合金涂层NiSiC比单纯的镍基合金涂层Ni60具有较高的硬度和耐磨性。     

武汉市长丰低闸施工期安全监测资料分析

对武汉市长江烂泥湖堤长丰低闸重建施工期间的安全监测资料进行了分析。重点分析了闸过水箱涵的结构缝开合、不均匀沉降等变形监测量变化特征及发展趋势 ,并由此对长丰低闸在施工期的安全状态进行了评判     

水溶性丙烯酸涂料的环氧树脂改性

本实验论述了水溶性丙烯酸钢铁底漆涂料制备、性能以及用环氧树脂对其进行改性。原水溶性丙烯酸钢铁底漆涂料在进行耐盐雾测定试验时仅为 72h ,未通过国家规定标准。本实验的目的在于 ,以丙烯酸树脂为主要成膜物质 ,加入其他的辅助成膜物质 ,如环氧树脂来改善其抗盐雾、抗腐蚀、耐候等性能。结果表明 ,一定温度下在水溶性丙烯酸钢铁底漆涂料中加入 1/ 7的环氧树脂后 ,经测定其耐盐雾性可达 10 6h ,达到了国家的规定标准     

基于CDMA智能网平台的增值业务--"位置服务"

本主要介绍基于CDMA智能网平台的一种增值业务——“位置服务”，阐述这个业务的网络结构、涉及到的技术问题和解决方案，并将这个整体思路投入到开发业务中去。     

船闸直立墙基坑开挖与锚固施工程序

三峡船闸深槽开挖与锚固工程量大，工期紧，施工强度高，施工难度大。因此，合理的施工程序有利于边坡稳定和施工，是快速和高质量施工的关键。分析了永久船闸直立墙基坑开挖与锚固施工程序的复杂性和进行研究的必要性。介绍了设计阶段研究船闸开挖与锚固的施工程序。对施工过程中研究施工程序时考虑的因素，研究的主要内容以及实际采用的施工程序作了简要的分析，归纳和总结。