互穿网络水凝胶角膜接触镜材料的合成及性能

通过高聚物分子设计的方法,运用互穿网络(IPN)技术,以吸水倍率为1.7的甲基丙烯酸β-羟乙酯(HEMA)水凝胶为基体材料,以吸水倍率为7~8的交联N-乙烯基吡咯烷酮(PVPP)颗粒为分散相,制备出了互穿程度不同的复合水凝胶角膜接触镜材料。利用SEM和TG分析仪对合成物的结构及热稳定性进行了表征。结果表明,m(PVPP颗粒)∶m(单体HEMA)=10∶90,以w(甘油或PEG400)=35%~45%作致孔剂,通过控制液体HEMA在PVPP颗粒中的渗透程度,可制得膨润率2%~5.3%、拉伸强度430~695 kPa、透光率>92%、含水质量分数45%左右的中等含水量的复合水凝胶角膜接触镜材料。DTG分析发现,在20~40℃,复合水凝胶的脱水速率随着互穿网络程度的提高而降低。     

Activation of Piezo1 Increases Na,K-ATPase-Mediated Ion Transport in Mouse Lens

Lens ion homeostasis depends on Na,K-ATPase and NKCC1. TRPV4 and TRPV1 channels, which are mechanosensitive, play important roles in mechanisms that regulate the activity of these transporters. Here, we examined another mechanosensitive channel, piezo1, which is also expressed in the lens. The purpose of the study was to examine piezo1 function. Recognizing that activation of TRPV4 and TRPV1 causes changes in lens ion transport mechanisms, we carried out studies to determine whether piezo1 activation changes either Na,K-ATPase-mediated or NKCC1-mediated ion transport. We also examined channel function of piezo1 by measuring calcium entry. Rb uptake was measured as an index of inwardly directed potassium transport by intact mouse lenses. Intracellular calcium concentration was measured in Fura-2 loaded cells by a ratiometric imaging technique. Piezo1 immunolocalization was most evident in the lens epithelium. Potassium (Rb) uptake was increased in intact lenses as well as in cultured lens epithelium exposed to Yoda1, a piezo1 agonist. The majority of Rb uptake is Na,K-ATPase-dependent, although there also is a significant NKCC-dependent component. In the presence of ouabain, an Na,K-ATPase inhibitor, Yoda1 did not increase Rb uptake. In contrast, Yoda1 increased Rb uptake to a similar degree in the presence or absence of 1 µM bumetanide, an NKCC inhibitor. The Rb uptake response to Yoda1 was inhibited by the selective piezo1 antagonist GsMTx4, and also by the nonselective antagonists ruthenium red and gadolinium. In parallel studies, Yoda1 was observed to increase cytoplasmic calcium concentration in cells loaded with Fura-2. The calcium response to Yoda1 was abolished by gadolinium or ruthenium red. The calcium and Rb uptake responses to Yoda1 were absent in calcium-free bathing solution, consistent with calcium entry when piezo1 is activated. Taken together, these findings point to stimulation of Na,K-ATPase, but not NKCC, when piezo1 is activated. Na,K-ATPase is the principal mechanism responsible for ion and water homeostasis in the lens. The functional role of lens piezo1 is a topic for further study.     

TGF-β/Smad Signalling Activation by HTRA1 Regulates the Function of Human Lens Epithelial Cells and Its Mechanism in Posterior Subcapsular Congenital Cataract

Congenital cataract is the leading cause of blindness among children worldwide. Patients with posterior subcapsular congenital cataract (PSC) in the central visual axis can result in worsening vision and stimulus deprivation amblyopia. However, the pathogenesis of PSC remains unclear. This study aims to explore the functional regulation and mechanism of HTRA1 in human lens epithelial cells (HLECs). HTRA1 was significantly downregulated in the lens capsules of children with PSC compared to normal controls. HTRA1 is a suppression factor of transforming growth factor-β (TGF-β) signalling pathway, which plays a key role in cataract formation. The results showed that the TGF-β/Smad signalling pathway was activated in the lens tissue of PSC. The effect of HTRA1 on cell proliferation, migration and apoptosis was measured in HLECs. In primary HLECs, the downregulation of HTRA1 can promote the proliferation and migration of HLECs by activating the TGF-β/Smad signalling pathway and can significantly upregulate the TGF-β/Smad downstream target genes FN1 and α-SMA. HTRA1 was also knocked out in the eyes of C57BL/6J mice via adeno-associated virus-mediated RNA interference. The results showed that HTRA1 knockout can significantly upregulate p-Smad2/3 and activate the TGF-β/Smad signalling pathway, resulting in abnormal proliferation and irregular arrangement of lens epithelial cells and leading to the occurrence of subcapsular cataract. To conclude, HTRA1 was significantly downregulated in children with PSC, and the downregulation of HTRA1 enhanced the proliferation and migration of HLECs by activating the TGF-β/Smad signalling pathway, which led to the occurrence of PSC.     

Nitro Dihydrocapsaicin,a Non-Pungent Capsaicin Analogue,Inhibits Cellular Senescence of Lens Epithelial Cells via Upregulation of SIRT1

Diabetic cataracts are a common complication that can cause blindness among patients with diabetes mellitus. A novel nitro dihydrocapsaicin (NDHC), a capsaicin analog, was constructed to have a non-pungency effect. The objective of this research was to study the effect of NDHC on human lens epithelial (HLE) cells that lost function from hyperglycemia. HLE cells were pretreated with NDHC before an exposure to high glucose (HG) conditions. The results show that NDHC promoted a deacceleration of cellular senescence in HLE cells. This inhibition of cellular senescence was characterized by a delayed cell growth and lower production of reactive oxygen species (ROS) as well as decreased SA-β-galactosidase activity. Additionally, the expression of Sirt1 protein sharply increased, while the expression of p21 and phospho-p38 proteins decreased. These findings provide evidence that NDHC could exert a pharmacologically protective effect by inhibiting the senescence program of lens cells during diabetic cataracts.     

Soemmerring’s Rings Developed around IOLs,in Human Donor Eyes,Can Present Internal Transparent Areas

Soemmerring’s rings consist of a ring of lens epithelial derived cells that grow along the periphery of an aphakic lens capsule, or around an intraocular lens. These rings when visualized frontally, appear opaque, however, in some cases the cells that compose these rings are organized in the same fashion as those in normal transparent adult lenses. Thus, our purpose was to test whether any part of the adult Soemmerring’s ring could be transparent and how this related to morphological factors. To study this, 16 Soemmerring’s rings were extracted from donor eye globes. After imaging, they were thickly sectioned sagittally in order to analyze the degrees of transparency of different areas. All samples were also histologically analyzed using alpha smooth muscle actin, Vimentin, wheat germ agglutinin and DAPI. Our results showed that many samples had some transparent areas, mostly towards the center of their cross-section. Of the factors that we analyzed, only lens fiber organization at the bow region and an increased area of mature lens fiber cells had a significant relation to the degree of transparency at the center. Thus, we can conclude that as Soemmerring’s rings mature, they can develop organized and transparent areas of lens cells.     

激光靶散射光观测镜杂散光抑制研究

分析了系统杂散光的来源,得出非成像光束对系统影响较大、平板玻璃窗产生的鬼像不容忽视的结论.针对传统作图法设计遮光罩的针对性不强,且追迹不同入射角的光线繁琐、容易遗漏的问题,本文提出一种逆向追迹法,依据光的可逆性,在像面处设置点阵面光源,每一点都发出充满系统孔径的光束,在物面设置足够大的探测面,使之有充足的余量记录所有到达物方的光线,利用该方法可以在探测面上直观她区分出非成像杂散光.有针对性地设计了具有特殊要求的内外遮光罩,并对遮光效果进行了软件模拟.     

低耦合损耗的光电混合光纤旋转连接器

设计了一种光电混合光纤旋转连接器,能实现相对旋转的光信号在较大对准误差范围内低损耗连接.旋转状态下的自聚焦透镜准直光纤输出的光信号,并由PIN光电探测器将其转换为电信号,冉由激光器根据电信号再生出原始光信号继续在光纤通讯系统中传输.该光电混合光纤旋转连接器在离轴偏移量至520μm或对准倾斜角至0.5°时的附加耦合损耗为0.3 dB,而采用双自聚焦透镜的光纤旋转连接器要获得小于3 dB的插入损耗,其离轴偏移或倾斜角度必须小于100 μm和0.10°相比之下,本文设计的光纤旋转连接器能降低系统对机械加上及装配精度的要求,具有较高的实用价值.     

针对大规模无线多跳网络的鱼眼机会路由协议

考虑到无线网络中广播特性、丢包特性、节点移动等对网络性能的影响,提出一种针对大规模无线多跳网络的鱼眼机会路由协议。在机会路由的基础上加入鱼眼技术,减少链路状态更新信息。在鱼眼路由表中加入链路传输概率,得到转发列表,并且简化机会路由协议的报文头结构,从而实现高效的数据传输。NS2仿真结果表明,该协议能降低网络端到端延时,提高网络吞吐量。     

远心镜头在回转体测量系统中的应用

介绍了一种接触测量和非接触测量相结合的回转体测量系统,分析了远心镜头在非接触测量部分的应用。阐述了回转体测量系统的结构和测量方案;讨论了待测回转体的特性参数,指出为了提高测量速度需尽可能采用非接触测量方法;使用远心镜头和平行光源进行了相关实验。结果表明:远心镜头的平行投影能有效地解决回转体工件平面特征成像轮廓线的干扰问题。     

LD抽运复合YAG晶体温度场及热透镜效应研究

为了研究复合Nd:YAG激光晶体棒在端面抽运下的温度分布及热透镜效应,采用数值分析法得到了端面抽运激光晶体棒内温度场的数值解,当抽运光功率为18W、光斑半径0.2mm时,吸收系数为3.5/cm的复合晶体内最大温度为73.8℃;同时还得到了径向梯度折射率引起的轴向传播光相位延迟分布ΔΦt(r),并与相同条件下的普通晶体作了比较.结果表明,采用复合晶体可以大幅度降低激光晶体内的温升,并减小端面变形导致的透镜效应;复合晶体内的相位延迟分布ΔΦt(r)与普通晶体的相似,且不满足对半径r的二次方关系;热透镜和参考球面镜透镜的相位延迟分布之间存在畸变Δφ,在r较小(rp)时,相位延迟分布ΔΦt(r)可近似等效于一球面透镜,其等效焦距fGRIN值与普通晶体的接近,在r较大(r>wp)时,畸变Δφ随r增大而急剧增大.