基于多Agent的大规模定制产品协同设计

提出了一个基于多Agent的智能化交互模型,拓展了产品集成协同设计系统(PICDS)的应用功能。探讨了系统实现的若干关键技术和实时协同交互手段。细化了客户、供应商和主设计部门三者之间面向大规模定制的协作机制,同时得出了分布式协同设计三阶段说这一理论。     

基于有限元法的柔性转子虚拟动平衡研究

为了有效地降低回转机械由于质量不平衡引起的振动故障，基于转子动力学理论和有限元数值分析方法，进行了柔性转子系统虚拟动平衡研究。提出了若干种降低系统振动的平衡方案，并分别对平衡结果进行了有限元数值仿真，以寻找最优的平衡方案。实验验证了虚拟动平衡提出的最优平衡方案，该方法可最大限度地降低旋转机械系统实际动平衡过程的启动试机次数。     

基于专家CAD平台的坐标测量机实例库技术与面向MC的产品设计

基于专家CAD平台的坐标测量机实例库技术以设计单元实例类为核心,用设计单元的分级层次结构组织实例库。实例类以设计单元为载体,是设计单元的抽象,提供了设计单元的属性及检索、修改等方面的信息。该设计单元实例库以商用CAD系统与专家系统集成构成的专家CAD系统作为平台。在该系统的GUI下,设计人员的经验和启发式思维方式直接面向专家系统,通过实例检索和约束设计进行设计单元实例的修改,方便快捷地完成定制产品的设计。该系统在一定程度上实现了坐标测量机面向大规模定制制造的设计理念。     

含H_2O气氛下Cu液中Ti的氧化SCIEI

本文研究了1500℃H_2O气氛下液态Cu合金中Ti的氧化。     

Mass Spectrometry-Based Disulfide Mapping of Lysyl Oxidase-like 2

Lysyl oxidase-like 2 (LOXL2) catalyzes the oxidative deamination of peptidyl lysines and hydroxylysines to promote extracellular matrix remodeling. Aberrant activity of LOXL2 has been associated with organ fibrosis and tumor metastasis. The lysine tyrosylquinone (LTQ) cofactor is derived from Lys653 and Tyr689 in the amine oxidase domain via post-translational modification. Based on the similarity in hydrodynamic radius and radius of gyration, we recently proposed that the overall structures of the mature LOXL2 (containing LTQ) and the precursor LOXL2 (no LTQ) are very similar. In this study, we conducted a mass spectrometry-based disulfide mapping analysis of recombinant LOXL2 in three forms: a full-length LOXL2 (fl-LOXL2) containing a nearly stoichiometric amount of LTQ, Δ1-2SRCR-LOXL2 (SRCR1 and SRCR2 are truncated) in the precursor form, and Δ1-3SRCR-LOXL2 (SRCR1, SRCR2, SRCR3 are truncated) in a mixture of the precursor and the mature forms. We detected a set of five disulfide bonds that is conserved in both the precursor and the mature recombinant LOXL2s. In addition, we detected a set of four alternative disulfide bonds in low abundance that is not associated with the mature LOXL2. These results suggest that the major set of five disulfide bonds is retained post-LTQ formation.     

Insight into the Impact of Oxidative Stress on the Barrier Properties of Lipid Bilayer Models

As a new field of oxidative stress-based therapy, cold physical plasma is a promising tool for several biomedical applications due to its potential to create a broad diversity of reactive oxygen and nitrogen species (RONS). Although proposed, the impact of plasma-derived RONS on the cell membrane lipids and properties is not fully understood. For this purpose, the changes in the lipid bilayer functionality under oxidative stress generated by an argon plasma jet (kINPen) were investigated by electrochemical techniques. In addition, liquid chromatography-tandem mass spectrometry was employed to analyze the plasma-induced modifications on the model lipids. Various asymmetric bilayers mimicking the structure and properties of the erythrocyte cell membrane were transferred onto a gold electrode surface by Langmuir-Blodgett/Langmuir-Schaefer deposition techniques. A strong impact of cholesterol on membrane permeabilization by plasma-derived species was revealed. Moreover, the maintenance of the barrier properties is influenced by the chemical composition of the head group. Mainly the head group size and its hydrogen bonding capacities are relevant, and phosphatidylcholines are significantly more susceptible than phosphatidylserines and other lipid classes, underlining the high relevance of this lipid class in membrane dynamics and cell physiology.     

Identification of Structural and Molecular Signatures Mediating Adaptive Changes in the Mouse Kidney in Response to Pregnancy

Pregnancy is characterized by adaptations in the function of several maternal body systems that ensure the development of the fetus whilst maintaining health of the mother. The renal system is responsible for water and electrolyte balance, as well as waste removal. Thus, it is imperative that structural and functional changes occur in the kidney during pregnancy. However, our knowledge of the precise morphological and molecular mechanisms occurring in the kidney during pregnancy is still very limited. Here, we investigated the changes occurring in the mouse kidney during pregnancy by performing an integrated analysis involving histology, gene and protein expression assays, mass spectrometry profiling and bioinformatics. Data from non-pregnant and pregnant mice were used to identify critical signalling pathways mediating changes in the maternal kidneys. We observed an expansion of renal medulla due to proliferation and infiltration of interstitial cellular constituents, as well as alterations in the activity of key cellular signalling pathways (e.g., AKT, AMPK and MAPKs) and genes involved in cell growth/metabolism (e.g., Cdc6, Foxm1 and Rb1) in the kidneys during pregnancy. We also generated plasma and urine proteomic profiles, identifying unique proteins in pregnancy. These proteins could be used to monitor and study potential mechanisms of renal adaptations during pregnancy and disease.     

The improved neutral network and its application for valuing rock mass mechanical parameter

The artificial neutral network（ANN） has the ability that self-study and self-remember, its 3 layers BP network has been applied extensively, but sometimes because of serious multi-correlation between the variables, and a few observations while many variables, there usually will result into paralyzing in study, and the neutral network further development is restricted in the system to some extent. The partial least square regression（PLS） has its advantage of building the calculation model between the variables with strong multi-correlation, especially much effective on a few data and many variables. So a new and effective method-improved neutral network has been introduced-the neutral network based on the PLS. The results of example show the improved method has a few calculations and high accuracy, and provide a new way for valuing the rock mass mechanical parameters.     

Rapid and Sensitive Liquid Chromatography-Tandem Mass Spectrometry for Quantification of Glycyrrhetic Acid in Human Plasma

A simple, rapid and sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) for the determination of glycyrrhetic acid in human plasma with ginsenoside Rh2 as internal standard was developed and validated. The plasma samples were prepared via liquid-liquid extraction with ethyl acetate. Chromatographic separation was accomplished on a Venusil MP-C18(50 mm×2.1 mm, 5μm i.d.) column at 25℃. The mobile phase consisted of acetonitrile/5 mmol·L-1 ammonium acetate(10:90, volume ratio) at a flow rate of 0.4 mL/min. Negative electrospray ionization was utilized as the ionization source. Glycyrrhetic acid and internal standard were determined via the mutiple reaction monitoring of precursor→production ion transitions at m/z 469→425, 409 and m/z 621→161,respectively. Each sample was chromatographed within 2.5 min. The lower limit of uantification was 0.50 ng/mL for 200 μL of plasma sample and the linear range was from 0.50 ng/mL to 800 ng/mL. The intra-and inter-day precisions were less than 8.76% in terms of relative standard deviation(RSD), and the accuracy was within a range of -3.25%-1.32% in terms of relative error(RE). The method was successfully applied to the pharmacokinetic studies of glycyrrhetic acid in healthy male Chinese volunteers after a single oral administration of 75 mg of glycyrrhizin.     

基于函数插值的柯氏质量流量计信号处理方法

对基于离散傅里叶变换(DFT)信号处理方式的柯氏质量流量计来说,影响其精度的关键问题是如何实现信号的整周期采样。提出了一种全新的方法,用采样率固定不变的采样数据,通过软件实时地确定信号的频率并且实现信号整周期重新采样。实验结果表明该方法在求取相位差时具有极高的精度。