Biochips are miniaturized, highly ordered analysis systems which offer the unique advantage of highly parallel analysis of thousands of analytes at the same time. Although this technique has been enthusiastically developed and has promised to improve and speed up numerous biological assays, the quality control of chip manufacture, chip analysis and data management has received less attention.
The following article compares three epoxy-containing chip surfaces (ARChip Epoxy, 3D-Link™, and EasySpot) with respect to their autofluorescence, immobilization capacity, background fluorescence and hybridization efficiency. Since data collected from biochip experiments are random snapshots with errors, inherently noisy and incomplete, we tried to evaluate technical factors causing variability and to set up quality control procedures for chip manufacture and chip analysis. Variabilities caused by arraying, glass substrate and polymer coating, fluorescent label and experimental conditions are discussed in details. 相似文献
This paper proposes a new approach for stabilizing a homogeneous solution in reaction–convection–diffusion system with oscillatory kinetics, in which moving or stationary patterns emerge in the absence of control. Specifically, we aim to suppress patterns by using a spatially weighted finite-dimensional feedback control that assures stability of the solution according to Lyapunov's direct method. A practical design procedure, based on spectral representation of the system and dissipative nature of parabolic PDEs, is presented. 相似文献
A decentralized stochastic control problem is called static if the observations available for any one decision do not depend
on the other decisions. Otherwise it is called dynamic. We consider only problems with a finite number of decisions. A notion
of equivalence between problems, suitable for complexity analysis, is defined. It turns out that a large class of dynamic
problems can be reduced to equivalent static problems. The class includes all sequential discrete variable problems and some
of the most studied continuous variable problems. 相似文献
An application of expert hierarchical control is described in this paper. The control is implemented in a two-level configuration, where the lower layer performs direct regulation control and the upper layer performs supervisory functions. In the regulation layer, a rule-based controller performs the regulation task, where the controller is constructed upon causal relations between subsystems. The control action is inferred from the measurement of both controlled and noncontrolled variables. In the supervisory layer, the main function is a fault diagnosis system which diagnoses faults on-line. The diagnosis is based upon reasoning from the structure of the system and the functions of its components, and efficient diagnosis is achieved by dividing the system into several subsystems. The overall technique has been successfully implemented on a pilot scale mixing process under on-line computer control. 相似文献