Accumulation of amplified target DNAs using thiol/biotin labeling, S1 nuclease, and ferrocene-streptavidin-magnetic system and a direct detection of specific DNA signals with screen printed gold electrode

Combinations of PCR-based amplification platform using 5′ thiolated and biotinylated specific primers, S1 nuclease-PCR products treatment, ferrocene-streptavidin (Fc-Stv)-magnetic binding for DNA accumulation, and screen printed gold electrode for the DNA allocation, were applied to Hoechst 33258-induced DNA aggregation and signals induction system for direct signals detection and DNA quantification in food samples. Thiolated and biotinylated at each 5′ terminus enabled DNA purification through S1 nuclease treatment for primers and non-specific DNA elimination and enabled DNA trapping with a ferrocene-streptavidin-magnetic system. This facilitated the accumulation of target DNAs at higher concentration, resulting in enhanced signals. After allocation of DNA on the surface of gold electrode via thiol binding, intensity of DNA signals through these treatments could be measured directly after being induced by Hoechst 33258. Wider amplitude changes in anodic current peaks between negative and positive samples (increasing from 3.70 to 10.10 μA) compared with those applied with no treatment combinations (decreasing from 3.92 to 1.23 μA) were observed. This enhancement of the signals allowed a greater efficiency of DNA quantification. When this combination was used for GMOs content estimation in reference samples, results revealed an improved accuracy from 66% to 96%. The combined biosensor system, although more costly than the standard Hoechst 33258/carbon electrode system, provided an alternative choice for DNA quantification, offering labor-free immobilization of probe onto electrode surface, easy test administration, and efficient semi-quantitative test without expensive instruments.     

Diffusion-controlled size-selective chloride ion sensing in the presence of bromide ion using a thin, nanoporous, plasma-polymerized coating on an Ag/AgCl electrode

We have developed a diffusion-controlled size-selective method for sensing chloride ion in the presence of bromide ion, based on a thin, nanoporous, plasma-polymerized coating of hexamethyldisiloxane on an Ag/AgCl electrode. Sub-nanometer-sized pores responsible for a highly cross-linked polymer network in the plasma-polymerized coating allowed diffusion-controlled permeation of chloride ion while blocking the larger bromide ion. An electrode coating of thickness greater than 70 nm enabled chloride detection in the concentration range 1-10 mM in the presence of 0.63 mM bromide ion. Advantages of this approach are: (1) simple design compared with ionophore-based strategies and (2) compatibility with microfabrication and mass production processes.     

非接触式叶片振测系统的叶尖定时传感器的研究

介绍了一种新型的叶尖定时传感器 ,传感器采用Y型耦合光纤与自聚焦透镜相结合 ,实现聚光照明及叶尖散射光的高效收集 ,大大提高了检测灵敏度。光源采用 4 5mW的半导体激光器 ,光电接收元件采用雪崩式光电二极管。传感器具有体积小、结构简单、成本低、安装和调节方便、测量精度高等优点。实验表明 ,该传感器对信号有良好的接收能力 ,满足涡轮机叶片高速、实时检测的要求 ,能为后续处理电路提供稳定而可靠的信号     

MAX6625型温度传感器的原理及应用

MAX6625是美国Maxim公司生产的一种新型智能温度传感器,它在风扇控制、温度告警、系统温度测量及工业设备中得到了广泛应用.介绍MAX6625的功能和结构,分析MAX6625的工作原理,给出采用MAX6625进行温度测控的电路及编程.     

Location and calculation-free node-scheduling schemes in large wireless sensor networks

In wireless sensor networks that consist of a large number of low-power, short-lived, unreliable sensors, one of the main design challenges is to obtain long system lifetime without sacrificing system original performance (sensing coverage and sensing reliability). To solve this problem, one of the potential approaches is to identify redundant nodes at the sensing interface and then assign them an off-duty operation mode that has lower energy consumption than the normal on-duty mode. In our previous work [J. Wireless Commun. Mobile Comput. 3 (2003) 271; Processing of ACM Wireless Sensor Network and Application Workshop 2002, September 2002], we proposed a node-scheduling scheme, which can provide a 100% sensing coverage preservation capability. This, however, requires each node to be aware of its own and its neighbors’ location information. Also, in that scheme, each node has to do accurate geometrical calculation to determine whether to take an off-duty status. In this paper, we propose and study several alternative node-scheduling schemes, which cannot completely preserve the original system coverage, but are nonetheless light-weighted and flexible compared with the previous one. Our simulation results compare these schemes with the previous one and demonstrate their effectiveness.     

基于多代理技术的传感器管理系统

针对分布式对空监视网中混合式传感器管理结构存在的问题，提出了基于多代理技术的混合式传感器管理结构。该结构较好地克服了混合式管理结构中在融合中心存在的瓶颈问题，通过多个传感器代理、融合中心代理之间的相互协商来实现传感器任务的分配。传感器代理与传感器相结合使传感器的工作模式实现高度的自动化和模块化，便于传感器的灵活组网。     

空间交会对接用激光雷达敏感器

本文讨论了在航天飞行器的空间交会对接(RVD)技术中采用激光雷达敏感器的可行性。提出在航天飞行器的自主交会对接技术中,应采用一种简单、实用的单目标系统。     

电阻式电压传感器结构设计及误差特性研究

从路的角度分析了电阻式电压传感器的误差特性 ,从场的角度比较了几种结构方案的幅值误差、相角误差及最大场强 ,优化出最佳方案 ,并分析了该方案幅值误差的频率特性     

Relationship between CO2 Sensor Voltage Response and Phase Equilibrium of Solid Electrolyte Na, K-β/β"-Al2O3

A type of CO2 sensor based on oxygen concentration cell was designed as following: Cell I: Pt | Au, O2,CO2|Na2CO3(Au)|NKBA(Au)|YSZ|O2, CO2|Pt or Cell Ⅱ: Pt|Au, O2, CO2|K2CO3(Au)|NKBA(Au)|YSZ|O2, CO2|Pt.(Na,K-β/β″-Al2O3 is named by NKBA). The sensor signal is consistent with the Nernstian slope within the region ofphase equilibrium for Na, K-β/β"-Al2O3 material. The relationship between CO2 sensor voltage response and phaseequilibrium of solid electrolyte Na, K-β/β-Al2O3 is discussed in this paper.     

计量秤在变角度状态的实践

介绍了物料计量秤在变角度下如何计算重量，以及变角度显示仪的工作原理和在实际中的运用。