Longwall mining automation horizon control:Coal seam gradient identification using piecewise linear fittingOA

Horizon control, maintaining the alignment of the shearer’s exploitation gradient with the coal seam gradient, is a key technique in longwall mining automation. To identify the coal seam gradient, a geological model of the coal seam was constructed using in-seam seismic surveying technology. By synthesizing the control resolution of the range arm and the geometric characteristics of the coal seam, a gradient identification method based on piecewise linear representation(PLR) is proposed. To achi...     

中山纪念堂屋面渗漏原因分析与治理方法

分析了中山纪念堂屋面的构造和渗漏的原因,介绍了屋面的维修原则和具体的整治方法。     

振动功率流方法诊断梁的损伤

利用振动功率流方法对无限直梁在集中力作用下的损伤进行了诊断研究。损伤部位模拟为转动弹簧,利用断裂力学的有关理论得到其转动刚度。研究了梁在集中载荷作用下的弯曲波运动以及振动功率流的输入和传播,分析了振动功率流与破损位置及其特征尺寸的关系。首先从理论上研究了破损诊断的具体步骤,然后进行了实验研究,结果证明了理论分析的准确性和可靠性。     

Oxidative Damage in Sporadic Colorectal Cancer: Molecular Mapping of Base Excision Repair Glycosylases MUTYH and hOGG1 in Colorectal Cancer Patients

Oxidative stress, oxidative DNA damage and resulting mutations play a role in colorectal carcinogenesis. Impaired equilibrium between DNA damage formation, antioxidant status, and DNA repair capacity is responsible for the accumulation of genetic mutations and genomic instability. The lesion-specific DNA glycosylases, e.g., hOGG1 and MUTYH, initiate the repair of oxidative DNA damage. Hereditary syndromes (MUTYH-associated polyposis, NTHL1-associated tumor syndrome) with germline mutations causing a loss-of-function in base excision repair glycosylases, serve as straight forward evidence on the role of oxidative DNA damage and its repair. Altered or inhibited function of above glycosylases result in an accumulation of oxidative DNA damage and contribute to the adenoma-adenocarcinoma transition. Oxidative DNA damage, unless repaired, often gives rise G:C > T:A mutations in tumor suppressor genes and proto-oncogenes with subsequent occurrence of chromosomal copy-neutral loss of heterozygosity. For instance, G>T transversions in position c.34 of a KRAS gene serves as a pre-screening tool for MUTYH-associated polyposis diagnosis. Since sporadic colorectal cancer represents more complex and heterogenous disease, the situation is more complicated. In the present study we focused on the roles of base excision repair glycosylases (hOGG1, MUTYH) in colorectal cancer patients by investigating tumor and adjacent mucosa tissues. Although we found downregulation of both glycosylases and significantly lower expression of hOGG1 in tumor tissues, accompanied with G>T mutations in KRAS gene, oxidative DNA damage and its repair cannot solely explain the onset of sporadic colorectal cancer. In this respect, other factors (especially microenvironment) per se or in combination with oxidative DNA damage warrant further attention. Base excision repair characteristics determined in colorectal cancer tissues and their association with disease prognosis have been discussed as well.     

Multiplexed-Based Assessment of DNA Damage Response to Chemotherapies Using Cell Imaging Cytometry

The current methods for measuring the DNA damage response (DDR) are relatively labor-intensive and usually based on Western blotting, flow cytometry, and/or confocal immunofluorescence analyses. They require many cells and are often limited to the assessment of a single or few proteins. Here, we used the Celigo^® image cytometer to evaluate the cell response to DNA-damaging agents based on a panel of biomarkers associated with the main DDR signaling pathways. We investigated the cytostatic or/and the cytotoxic effects of these drugs using simultaneous propidium iodide and calcein-AM staining. We also describe new dedicated multiplexed protocols to investigate the qualitative (phosphorylation) or the quantitative changes of eleven DDR markers (H2AX, DNA-PKcs, ATR, ATM, CHK1, CHK2, 53BP1, NBS1, RAD51, P53, P21). The results of our study clearly show the advantage of using this methodology because the multiplexed-based evaluation of these markers can be performed in a single experiment using the standard 384-well plate format. The analyses of multiple DDR markers together with the cell cycle status provide valuable insights into the mechanism of action of investigational drugs that induce DNA damage in a time- and cost-effective manner due to the low amounts of antibodies and reagents required.     

Numerical Simulation of Brittle Damage in Concrete Specimens

A framework for damage mechanics of concrete is applied to simulate the nonlinear elastic deformation behavior of concrete using finite element method (FEM). A rather simple isotropic damage model containing essentially no adjustable parameters is shown to produce results in remarkably good agreement with sample experimental data: the damage law requires only the fracture energy to be defined completely. The model is achieved by introducing a damage surface that is similar to the yield function in the conventional theory of plasticity. A special form of damage surfaces is constructed to illustrate the application of the model. A new damage criterion, defined as an equivalent strain norm, is proposed, in order to take into consideration the asymmetric behavior of concrete. For verifying the FEM program including the model, deformations predicted by this model are compared with both the experimental ones for the concrete structural model and the ones calculated without application of the continuum damage mechanics.__________Translated from Problemy Prochnosti, No. 3, pp. 57 – 74, May – June, 2005.     

Recombination luminescence from defects in boron-ion implantation-doped diamond using low fluences

 Two identical, high purity, natural type IIa diamonds, which displayed the ubiquitous blue cathodoluminescence (CL) band at ≈ 2.9 eV, as well as an indication of the corresponding green band at ≈ 2.4 eV, have been equivalently doped by using extremely low dose B⁺- and C⁺-ion CIRA-implantations respectively. Comparative CL measurements showed changes in the intensities of the 2.9 and 2.4 eV bands and the generation of bands at ≈ 4 eV, as well as at ≈ 3.5 and ≈ 4.6 eV (the latter two in the B⁺-CIRA diamond). The results are commensurate with the model (proposed previously) in which the 2.9 and 4 eV bands are generated respectively by electron-hole recombinations at negatively charged acceptor- and positively charged donor-like, intrinsic defects. The present results indicate that Coulomb interactions between the latter defects and (at least partially) compensated, negatively charged, boron acceptors, generate the 3.5 and 4.6 eV bands, which may be considered as higher energy (≈ 0.6 eV) replicas of the 2.9 and 4 eV bands. In both cases, two electrons and a hole interact just before the hole combines with an electron. Such a configuration of charges seems related to, and could possibly be described as, a type of ”ionised exciton molecule”, where the ”bonding” of two negative ”nuclei” is facilitated by the presence of the hole. The CL measurements further indicate that the 2.4 eV band forms when a high enough density of, in this case, neutral acceptors are present. These neutral acceptors compete with the valence band to supply holes for recombination at the negatively charged, acceptor-type, intrinsic defects which are, in the absence of the boron, responsible for the generation of the blue, 2.9 eV band. Received: 5 December 1997 / Accepted: 13 December 1997     

基于柔度差曲率矩阵的结构损伤识别方法

柔度是较频率和位移模态更敏感的结构损伤标示量。提出利用结构损伤前、后的柔度矩阵,先后对柔度矩阵差的列、行进行两次差分,求得柔度差曲率矩阵(δFlexibility Curvature Matrix),并以其对角元素作为检测结构损伤指标(δFCMD)的新方法。该方法仅需低阶模态参数即可进行损伤检测,不论对简支梁、悬臂梁、固支梁,或多跨连续梁,单一位置损伤、支撑处损伤、轻微损伤,还是多种损伤共存,均具有损伤定位的能力、并能定性反映损伤程度。通过与已有的柔度差、柔度变化率、均匀荷载面曲率差等柔度指标的数值模拟分析研究,显示了该指标检测损伤的有效性和优越性。     

急倾斜水平分段放顶煤放煤规律的离散元模拟研究

提高水平分段高度是提高急倾斜水平分段放顶煤产量的有效途径,但顶煤分段高度提高后,其顶煤的放出工艺如何控制是影响其放出率的关键因素。对急倾斜水平分段放顶煤的放煤过程进行了离散元数值模拟,重点比较了在相同顶煤高度的条件下,顺序放煤与隔架放煤的特点,并在理论分析中给出了确定放煤轮数的具体公式,所得结论可供工程实际参考应用。     

基于差分进化算法的面目标瞄准点寻优

瞄准点的寻优选取是导弹火力筹划的核心理论问题之一。针对此问题,本文基于矩形面目标毁伤面积计算设计评价函数,利用差分进化算法原理对面目标的瞄准点坐标进行实数编码,并设计差分进化算子,建立面目标瞄准点寻优模型。通过设计面目标计算实例对模型进行验证,实验结果表明,差分进化算法的稳定性较强且具有较好的操作性,模型求得的瞄准点可信度较高,能够提升导弹打击效果并降低打击成本,为火力筹划中瞄准点寻优提供了新方法。