EK100 and Antrodin C Improve Brain Amyloid Pathology in APP/PS1 Transgenic Mice by Promoting Microglial and Perivascular Clearance Pathways

Alzheimer’s disease (AD) is characterized by the deposition of β-amyloid peptide (Aβ). There are currently no drugs that can successfully treat this disease. This study first explored the anti-inflammatory activity of seven components isolated from Antrodia cinnamonmea in BV2 cells and selected EK100 and antrodin C for in vivo research. APPswe/PS1dE9 mice were treated with EK100 and antrodin C for one month to evaluate the effect of these reagents on AD-like pathology by nesting behavior, immunohistochemistry, and immunoblotting. Ergosterol and ibuprofen were used as control. EK100 and antrodin C improved the nesting behavior of mice, reduced the number and burden of amyloid plaques, reduced the activation of glial cells, and promoted the perivascular deposition of Aβ in the brain of mice. EK100 and antrodin C are significantly different in activating astrocytes, regulating microglia morphology, and promoting plaque-associated microglia to express oxidative enzymes. In contrast, the effects of ibuprofen and ergosterol are relatively small. In addition, EK100 significantly improved hippocampal neurogenesis in APPswe/PS1dE9 mice. Our data indicate that EK100 and antrodin C reduce the pathology of AD by reducing amyloid deposits and promoting nesting behavior in APPswe/PS1dE9 mice through microglia and perivascular clearance, indicating that EK100 and antrodin C have the potential to be used in AD treatment.     

1Cr18Ni9Ti不锈钢双辊薄带的铸态组织特征研究

通过双辊薄带连铸试验和组织成分分析,对1Cr18Ni9Ti不锈钢双辊薄带的铸态组织特征进行了研究。结果表明：1Cr18Ni9Ti不锈钢双辊薄带的铸态组织和传统薄板坯没有本质区别,但薄带坯组织较为细密,柱状晶区呈不严格的对称分布;一次枝晶和二次枝晶间距小,并且均随离薄带表面距离的增加而增大;柱状枝晶的生长比较紊乱,没有严格的方向性;在薄带厚度方向上元素铬和镍没有明显的成分偏析。     

稀土对9Cr2Mo钢抗回火能力的影响

通过在冷轧辊用９Ｃｒ２Ｍｏ钢中添加稀土元素,利用全自动膨胀仪研究了稀土元素对其抗回火能力的影响。试验结果证明,稀土元素对钢中碳化物的结构没有影响,但抑制了碳化物的析出过程,提高了其抗回火能力。     

Enlèvement du phosphore des eaux usées par traitement à base de tourbe dopée aux boues rouges

The addition of an aluminium and iron doping agent, bauxite red mud, has been tested with a commercial peat for thetreatment of a secondary municipal wastewater. Based on a column study, phosphorus (P) removal was raised from about17% to 21% on peat alone to over 95% with red mud treatment of peat. By this mean, the use of red muds allowed areduction of P, concentration effluent to below 0.15 mg/L. The boosting effect of red mud seemed to decrease with timeand especially with the hydraulic loading. For a hydraulic loading of 30 cm/j, P removal met govermental guidelines (_t effluent < 1 mg/L) for a period of 50 days. Other classic efficiency parameters such as BOD₅, COD and coliforms werenot altered by the use of the doping agent.     

煤矿瓦斯灾害风险隐患大数据监测预警云平台与应用

瓦斯灾害仍然是我国深部煤矿开采的主要灾害,随着采深及开采强度的加大,危险性日趋严重.瓦斯灾害影响因素及耦合关系非常复杂,灾害事故时有发生.基于安全监测大数据进行瓦斯灾害和风险隐患一体化分析预警已成为煤矿安全管理、监管和监察的迫切需求.分析了瓦斯灾害与风险隐患的大数据特征,提出了基于安全监测大数据的瓦斯灾害风险隐患识别与...     

碳九芳烃的综合利用

本文重点介绍了国内乙烯副产品碳九芳烃的资源状况及碳九石油树脂的生产、应用情况。指出了碳九芳烃综合利用的途径及发展方向。     

Co-based zeolitic imidazolate framework ZIF-9 membranes prepared on α-Al2O3 tubes through covalent modification for hydrogen separation

Hydrogen has been regarded as the most promising clean and renewable energy. Beside the production of the hydrogen, the separation of hydrogen is also an import issue before it can be used in fuel cells. Membrane-based separation technologies have gained considerable attentions due to its high efficiency and low energy consumption. Zeolite imidazolate framework (ZIF) membranes have drawn intense interest due to their zeolite-like properties such as permanent porosity, uniform pore size and exceptional thermal and chemical stability. It is rather challenged to prepare well-intergrown Co-based zeolitic imidazolate frameworks (ZIFs) membranes on porous α-Al₂O₃ tubes since Co-based ZIFs prefer to form crystals in the synthesis solution rather than grow as membrane layer on the support surface. In this work, we report the preparation of high-quality ZIF-9 membrane with high H₂/CO₂ selectivity and excellent thermal stability by using 3-aminopropyltriethoxysilane (APTES) as a covalent linker to modify the α-Al₂O₃ tube. Due to the formation of covalent bonds between APTES and ZIF-9, ZIF-9 nutrients are bound to the support surface, thus promoting the growth of dense and phase-pure ZIF-9 membrane with a thin thickness of about 4.0 μm. The gas separation performances of the ZIF-9 membrane were evaluated by single gas permeation and mixture gas separation of H₂/CO₂, H₂/N₂ and H₂/CH₄, respectively. The mixture separation factors of H₂/CO₂, H₂/CH₄, and H₂/N₂ of the ZIF-9 membrane are 21.5, 8.2 and 14.7, respectively, which by far exceeds corresponding Knudsen coefficients. Moreover, the as-prepared ZIF-9 membrane exhibits excellent stability at a relatively broad range of operating temperature, which is beneficial for the industrial application of hydrogen separation or further membrane reactor.     

手性配体1,4-双(9-O-奎宁)-2,3-二氮杂萘的简便合成方法

在NaH存在下,以DMF为溶剂,在温和条件下合成了手性配体1,4-双(9-O-奎宁)-2,3-二氮杂萘,最后用重结晶方法得到纯品,为大量合成该配体提供了可行的方法。     

Regulation of Epigenetic Modifications in the Placenta during Preeclampsia: PPARγ Influences H3K4me3 and H3K9ac in Extravillous Trophoblast Cells

The aim of this study was to analyze the expression of peroxisome proliferator-activated receptor γ (PPARγ) and retinoid X receptor α (RxRα), a binding heterodimer playing a pivotal role in the successful trophoblast invasion, in the placental tissue of preeclamptic patients. Furthermore, we aimed to characterize a possible interaction between PPARγ and H3K4me3 (trimethylated lysine 4 of the histone H3), respectively H3K9ac (acetylated lysine 9 of the histone H3), to illuminate the role of histone modifications in a defective trophoblast invasion in preeclampsia (PE). Therefore, the expression of PPARγ and RxRα was analyzed in 26 PE and 25 control placentas by immunohistochemical peroxidase staining, as well as the co-expression with H3K4me3 and H3K9ac by double immunofluorescence staining. Further, the effect of a specific PPARγ-agonist (Ciglitazone) and PPARγ-antagonist (T0070907) on the histone modifications H3K9ac and H3K4me3 was analyzed in vitro. In PE placentas, we found a reduced expression of PPARγ and RxRα and a reduced co-expression with H3K4me3 and H3K9ac in the extravillous trophoblast (EVT). Furthermore, with the PPARγ-antagonist treated human villous trophoblast (HVT) cells and primary isolated EVT cells showed higher levels of the histone modification proteins whereas treatment with the PPARγ-agonist reduced respective histone modifications. Our results show that the stimulation of PPARγ-activity leads to a reduction of H3K4me3 and H3K9ac in trophoblast cells, but paradoxically decreases the nuclear PPARγ expression. As the importance of PPARγ, being involved in a successful trophoblast invasion has already been investigated, our results reveal a pathophysiologic connection between PPARγ and the epigenetic modulation via H3K4me3 and H3K9ac in PE.     

Functional Studies of Plant Latex as a Rich Source of Bioactive Compounds: Focus on Proteins and Alkaloids

Latex, a sticky emulsion produced by specialized cells called laticifers, is a crucial part of a plant’s defense system against herbivory and pathogens. It consists of a broad spectrum of active compounds, which are beneficial not only for plants, but for human health as well, enough to mention the use of morphine or codeine from poppy latex. Here, we reviewed latex’s general role in plant physiology and the significance of particular compounds (alkaloids and proteins) to its defense system with the example of Chelidonium majus L. from the poppy family. We further attempt to present latex chemicals used so far in medicine and then focus on functional studies of proteins and other compounds with potential pharmacological activities using modern techniques such as CRISPR/Cas9 gene editing. Despite the centuries-old tradition of using latex-bearing plants in therapies, there are still a lot of promising molecules waiting to be explored.