The Evidence for Effective Inhibition of INa Produced by Mirogabalin ((1R,5S,6S)-6-(aminomethyl)-3-ethyl-bicyclo [3.2.0] hept-3-ene-6-acetic acid), a Known Blocker of CaV Channels

Mirogabalin (MGB, Tarlige^®), an inhibitor of the α₂δ-1 subunit of voltage-gated Ca²⁺ (Ca_V) channels, is used as a way to alleviate peripheral neuropathic pain and diabetic neuropathy. However, to what extent MGB modifies the magnitude, gating, and/or hysteresis of various types of plasmalemmal ionic currents remains largely unexplored. In pituitary tumor (GH₃) cells, we found that MGB was effective at suppressing the peak (transient, I_Na(T)) and sustained (late, I_Na(L)) components of the voltage-gated Na⁺ current (I_Na) in a concentration-dependent manner, with an effective IC₅₀ of 19.5 and 7.3 μM, respectively, while the K_D value calculated on the basis of minimum reaction scheme was 8.2 μM. The recovery of I_Na(T) inactivation slowed in the presence of MGB, although the overall current–voltage relation of I_Na(T) was unaltered; however, there was a leftward shift in the inactivation curve of the current. The magnitude of the window (I_Na(W)) or resurgent I_Na (I_Na(R)) evoked by the respective ascending or descending ramp pulse (V_ramp) was reduced during cell exposure to MGB. MGB-induced attenuation in I_Na(W) or I_Na(R) was reversed by the further addition of tefluthrin, a pyrethroid insecticide known to stimulate I_Na. MGB also effectively lessened the strength of voltage-dependent hysteresis of persistent I_Na in response to the isosceles triangular V_ramp. The cumulative inhibition of I_Na(T), evoked by pulse train stimulation, was enhanced in its presence. Taken together, in addition to the inhibition of Ca_V channels, the Na_V channel attenuation produced by MGB might have an impact in its analgesic effects occurring in vivo.     

Dynamic Changes in Reactive Oxygen Species in the Shoot Apex Contribute to Stem Cell Death in Arabidopsis thaliana

In monocarpic plants, stem cells are fated to die. However, the potential mechanism of stem cell death has remained elusive. Here, we reveal that the levels of two forms of reactive oxygen species (ROS), superoxide anion free radical (O2&#x<font color="red">000</font>b7;−) and hydrogen peroxide (H₂O₂), show dynamic changes in the shoot apex during the plant life cycle of Arabidopsis thaliana. We found that the level of O2&#x<font color="red">000</font>b7;− decreased and disappeared at four weeks after bolting (WAB), while H₂O₂ appeared at 3 WAB and showed a burst at 5 WAB. The timing of dynamic changes in O2&#x<font color="red">000</font>b7;− and H₂O₂ was delayed for approximately three weeks in clv3-2, which has a longer lifespan. Moreover, exogenous application of H₂O₂ inhibited the expression of the stem cell determinant WUSCHEL (WUS) and promoted the expression of the developmentally programmed cell death (dPCD) marker gene ORESARA 1 (ORE1). These results indicate that H₂O₂ triggers an important signal inducing dPCD in stem cells. Given that O2&#x<font color="red">000</font>b7;− plays roles in maintaining WUS expression and stem cell activity, we speculate that the dynamic shift from O2&#x<font color="red">000</font>b7;− to H₂O₂ in the shoot apex results in stem cell death. Our findings provide novel insights for understanding ROS-mediated regulation during plant stem cell death.     

40000kN液压支架试验台的研制

40 000 kN试验台是我公司自主创新研制的新型液压支架试验台,具有独立自主知识产权,是目前国内试验载荷参数最大,性能最先进的试验台,不仅为我国大工作阻力高端液压支架研制提供了试验条件,同时也填补了国内大型内加载液压支架试验台的空白。     

1000MW机组锅炉给水系统优化

通过介绍几种给水泵组常规的布置形式，提出了绥中发电厂二期工程的推荐方案——2台50％的汽动给水泵。然后从几个方面论述不设备用泵、采用本方案的可行性。     

近水平千米定向钻机在矿井瓦斯抽采中的应用

针对目前井下常规钻机能力偏小,瓦斯预抽期较短,矿井掘抽采比例严重失调的现象,采用VLD-1000型和ZDY6000LD型井下千米定向钻机,根据工作面掘进、回采接续和目标区域瓦斯赋存的特点设计、布置钻场钻孔。详细介绍了钻孔施工过程、施工情况及瓦斯抽采效果,千米定向钻进相比于传统常规钻进工艺,定向钻孔的单孔成孔距离、钻孔煤孔比例、瓦斯抽采浓度分别为原穿层钻孔的6.0、2.3、2.0倍,具有瓦斯抽采率高、预抽期延长、钻孔施工适应性强、钻孔覆盖范围大等特点。     

AP1000核电厂自动泄压系统误启动事故计算

基于最佳估算程序RELAP5/MOD3.3,对AP1000核电厂冷却剂系统和非能动堆芯冷却系统进行了建模分析,得到了自动泄压系统(ADS)阀门误启动事故下,系统压力、破口流量、系统水装量等参数的瞬态变化,计算结果与西屋公司采用NOTRUMP程序的计算结果进行了比较与分析。结果表明:AP1000核电厂的非能动专设安全设施能有效对一回路进行冷却和降压,防止堆芯过热,验证了AP1000发生ADS阀门误启动事故后的安全性。     

7000 m以深优质砂岩储层的特征、成因机制及油气勘探意义——以库车坳陷下白垩统巴什基奇克组为例

超深层碎屑岩储层物性一般极差,单井油气产能低,但塔里木盆地库车坳陷新近钻探的博孜9井却在7 600 m以深的下白垩统巴什基奇克组仍然钻遇了优质厚层储层,并获得了高产工业气流。为了揭示该储层的特征和成因、降低超深层油气勘探的风险,基于岩心、测井和实验分析等资料,结合区域温压条件和储层埋藏演化史,探讨了巴什基奇克组储层的特征、形成机制及其油气勘探意义。研究结果表明:①该区巴什基奇克组超深层储层岩石类型为中、细粒长石岩屑砂岩和岩屑长石砂岩,颗粒呈点—线接触,储集空间以原生粒间孔为主,现今仍处于中成岩演化阶段;②与其他超深层裂缝性低孔砂岩储层不同,该套超深层储层为孔隙型储层,孔隙度介于4%～13%,渗透率介于0.1～50.0 mD,孔渗相关性较好;③巴什基奇克组沉积期广泛发育三角洲前缘厚层砂体,中、细砂岩占比超过85%,颗粒抗压能力强,此后储层经历了早—中期长时间浅埋藏,晚期快速深埋,埋藏压实效应较弱,晚期上覆古近系巨厚膏盐岩层受冲断挤压形成顶蓬构造,进一步抑制垂向压实,同时研究区远离造山带和构造转换带,侧向挤压应力弱,原生粒间孔得以大量保存。结论认为:粗岩性、弱压实和低构造应力是该套超深层优...     

16×104 m3LNG储罐罐顶气顶升工艺的计算分析

16×10⁴ m³LNG储罐是国际上LNG接收站存储系统的常用储罐,其制作一般采用气顶升工艺。该工艺施工速度快,成本相对较低,但影响因素多、施工难度高、安全风险大,国内项目以前均是由国外工程公司总承包,气顶升方案也均由外方制订,没有给出详细的计算分析过程与步骤,目前,国内施工单位对该工艺的施工一般也是参照国外通俗做法,鲜有详细的计算分析。为此,对大连LNG项目16×10⁴ m³LNG储罐罐顶气顶升工艺方案的供气装置和密封装置进行了计算分析,确定了16×10⁴ m³LNG储罐的施工方案,并以计算分析结果为依据指导实际施工,收效良好,对类似大型LNG储罐罐顶气顶升工艺的设计具有一定参考价值。