考马斯亮蓝G250染色法测定啤酒中蛋白质含量

以啤酒为原料,利用蛋白质与考马斯亮蓝显色原理,测定啤酒中蛋白质含量,并对测定条件进行优化研究,实验结果表明：波长在595nm处蛋白质有最大吸收峰,反应时间3～5min为最适宜,SDS对马斯亮蓝测定蛋白质含量有干扰。测定所用的标准曲线方程为：v=0．1296x＋0．0907。考马斯亮蓝G-250法测量蛋白质含量与凯氏定氮法相比较误差范围3％～8％。     

浅析HPT250轧机中滑移系数对轧制过程的影响

通过HPT250轧机在技术改造中遇到的一个问题，即滑移系数对冷轧元缝钢管在轧制过程中所起的作用。在实际生产中经过不断的技术研究和改进。使闲置多年的HPT250轧机为钢管公司的生产迭拉起到很大的作用。     

The Unified Project

Albert Pope argues that a unified architectural and urban project is not possible without addressing the conceptual divide that exists between building and infrastructure. It is a split that was first set in motion by the breakdown of Modernism in the 1960s and was reaffirmed by the rise of Postmodernism. Since then, architectural form and urban infrastructure have not only remained disjointed, but have been overtly celebrated by the collages and juxtapositions of the contemporary and the historic in our cities.     

Effect of aging treatment on the mechanical properties of C-250 maraging steel by flow forming

The technique of forward flow forming has been used to produce a long, thin walled tube made of C-250 maraging steel. The forward flow forming can save raw material, increase strength, and reduce the production process time. Because the work hardening effect on solution-treated C-250 using flow forming is minimal, the flow-formed tube requires an additional heat treatment to obtain higher hardness and strength. With the direct aging treatment, low elongation values are obtained, making this treatment unsuitable for the engineering design. It was found that the 540 °C/6 h/AC over-aging treatment results in better strength and elongation values. The strengthening phase of the flow-formed C-250 maraging steel was found to be the intermetallic compound of Fe₃Mo.     

Photoelectrochemical observations on chlorophyll- carotene interactions in a lipid bilayer film

Photoelectrochemical investigations were undertaken on energy transfer and other interactions between chlorophyll-a (Chl-a) and β-carotene (Car) in lipid monolayers. A heterobilayer composed of a Chl-a-lecithin monolayer and a Car- lecithin monolayer was prepared on an SnO₂ semiconductor electrode by Langmuir-Blodgett techniques. When the bilayer-coated electrode was irradiated in an aqueous electrolyte, anodic photocurrents due to the sensitizations by Chl-a and Car respectively were observed. The photocurrent efficiency (ratio of photons to electrons) was much for Car (about 10^-3) than for Chl-a (about 10^-1). This large difference permits a quantitative estimation of the energy transfer from Car to Chl-a, based on the concept that the energy transfer results in an increase in the Car- induced sensitized photocurrent. The effect of added Chl-a on the Car photocurrent was measured for heterobilayers with various Chl-a-to-Car molar ratios. The experimental results, as well as theoretical calculations, showed that the Car-to- Chl-a energy transfer is inefficient. The effect of Car on the Chl-a-sensitized photocurrent was also studied with the same bilayer system. The effect was shown to be complex depending on the Car content. A lowering of the Chl-a photocurrent occurred at high Car contents, which is considered to reflect the quenching of the Chl-a excited triplet by Car. The usefulness of the present photoelectrochemical approach for the study of energy transfer between the photosynthetic pigments is demonstrated.     

基于Intel Xscale PXA250上个人数字助理

文中介绍Intel Xscale PXA250微处理器的软硬件平台,并详细给出在IPXA250上个人数字助理的开发应用原理.     

Measuring plant protein with the Bradford assay

The suitability of the Bradford protein assay for measuring plant protein was evaluated and a standard method developed. The assay involves extraction of dried, fresh, or frozen plant material in 0.1 NaOH for 30 min. Replicate 100-l aliquots of centrifuged supernatant are assayed with 5 ml Bio-Rad Bradford dye reagent (Coomassie brilliant blue G-250) diluted 1:4 and containing 3 mg/ml soluble polyvinylpyrollidone. Absorbance at 595 nm is recorded after 15 min against an NaOH blank. Samples are calibrated against a ribulose 1,5-diphosphate carboxylase-oxygenase standard in NaOH. Procedures for plant preparation, extraction stability, the effects of phenol removal and quinone formation, and assay recovery are evaluated. Assay absorbance stability and techniques for increasing absorbance stability are reported. Changes in protein quality are briefly discussed.     

T-250钢薄壁圆筒环缝电子束焊接数值模拟

利用ANSYS软件采用三维锥体热源模型对T-250钢薄壁圆筒环缝电子束焊接进行数值模拟。结果表明,三维锥体热源模型适合于电子束焊接的数值模拟,由此得出的温度场分布与实际焊接情况吻合良好。残余应力在距焊缝中心轴向4 mm区域内会发生突变或者达到最大值。这个区域正好对应实际焊接的焊缝区和热影响区,说明数值模拟与实际焊接有较好的对应性。     

钛及钛合金棒丝材轧制用的φ250轧机轧辊轴承改造

五机架横列式6250轧机的第一、二、三机架为三辊刑材轧机,采用传统的斜楔侧面固定方式固定轧辊,中辊固定稳定性差,在钛及钛合金轧制过程中尺寸波动幅度大、电耗高、备件更换频紧,因此将该轧机的斜楔侧向固定方式改为轴承固定结构。改造效果评估表明,随机抽取了φ250轧机改造前后轧制的φ23．5、φ18．5、φ12．5mm三个批次的钛及钛合金捧材,改造前尺寸波动范围为23．2～23．9mm、18．1～18．8mm、12.1～12．8mm,改造后尺寸波动范围23．6～23．8mm、18．5～18．8mm、12．5～12．8mm;改造前（2009年5—8月）每生产1t钛及钛合金棒材所消耗的电能为830．395kW·h,改造后（2010年5—10月）为585．603kW·h,电耗降低了29．5％;备件的使用费用由改造油的5．9万几降低到改造后的0．14万元。     

HT250铸件表面孔洞缺陷分析

采取硬度检测、托伸试验、金相检验和化学成分分析等方法,对不同铸造工艺HT250铸件的表面孔洞缺陷进行调查与分析.结果表明:HT250铸件硬度明显低于国家标准要求,部分铸件的抗拉强度不满足国家标准要求,金相组织中铁素体含量较高(珠光体含量低于85％),石墨粗大、不均匀,化学成分不满足企业标准要求.孔洞缺陷是其加工表面在高...