Dark hydrogen production in nitrogen atmosphere – An approach for sustainability by marine cyanobacterium Leptolyngbya valderiana BDU 20041

Biological hydrogen production is an ideal system for three main reasons i) forms a renewable energy source, ii) gives clean fuel and iii) serves as a good supplement to oil reserves. The major challenges faced in biological hydrogen production are the presence of uptake hydrogenase and lack of sustainability in the cyanobacterial hydrogen production system. Three different marine cyanobacterial species viz. Leptolyngbya valderiana BDU 20041, Dichothrix baueriana BDU 40481 and Nostoc calcicola BDU 40302 were studied for their potential use in hydrogen production. Among these, L. valderiana BDU 20041, was found to produce hydrogen even in 100% nitrogen atmosphere which was 85% of the hydrogen produced in argon atmosphere. This is the first report of such a high rate of production of hydrogen in a nitrogen atmosphere by a cyanobacterium, which makes it possible to develop sustained hydrogen production systems. L. valderiana BDU 20041, a dark hydrogen producer uses the reductant essentially supplied by the respiratory pathway for hydrogen production. Using inhibitors, this organism was found to produce hydrogen due to the activities of both nitrogenase and bidirectional hydrogenase, while it had no ‘uptake’ hydrogenase activity. The other two organisms though had low levels of bidirectional hydrogenase, possessed considerable ‘uptake’ hydrogenase activity and hence could not release much hydrogen either in argon or nitrogen atmosphere.     

Degradation of cyanobacteria toxin by advanced oxidation processes

Advanced oxidation processes (AOPs) using O(3), H(2)O(2), O(3)/H(2)O(2), O(3)/Fe(II), and Fenton treatment were investigated for the degradation of aqueous solutions of cyanobacteria. The effects of concentration of reactants, temperature, and pH on toxins degradation were monitored and the reaction kinetics was assessed. O(3) alone or combined with either H(2)O(2) or Fe(II) were efficient treatment for toxins elimination. A higher toxin oxidation tendency was observed with Fenton reaction; total toxins degradation (MC-LR and MC-RR) was achieved in only 60s. The ozonation treatment was successfully described by second-order kinetics model, with a first-order with respect to the concentration of either ozone or toxin. At 20 degrees C, with initial concentration of MC-LR of 1mg/L, the overall second-order reaction rate constant ranged from 6.79 x 10(4) to 3.49 x 10(3)M(-1)s(-1) as the solution pH increased from 2 to 11. The reaction kinetics of the other AOPs (O(3)/H(2)O(2), O(3)/Fe(II), and Fenton), were fitted to pseudo first-order kinetics. A rapid reaction was observed to took place at higher initial concentrations of O(3), H(2)O(2) and Fe(II), and higher temperatures. At pH 3, initial concentration of toxin of 1mg/L, the pseudo first-order rate constant, achieved by Fenton process, was in order of 8.76+/-0.7s(-1).     

Development and application of a molecular assay to detect and monitor geosmin-producing cyanobacteria and actinomycetes in the Great Lakes

Geosmin is a potent earthy-smelling sesquiterpene responsible for the majority of biologically induced taste-and-odor (T/O) episodes in drinking and recreational water, and major economic losses to commercial, farmed and sports fisheries. Geosmin is produced by a range of microorganisms, notably by cyanobacteria and actinomycetes. However, the effective management of geosmin T/O episodes has been hindered by our inability to identify the major biological sources, and absence of sensitive methods for early detection of T/O events. The main goal of this study was to develop taxon-specific PCR and RT-PCR assays that facilitate early detection of potential geosmin releases and identification of the likely biological sources. We developed a molecular assay for the detection and expression of geosmin synthase (geoA), a gene involved in geosmin biosynthesis in cyanobacteria and actinomycetes. Using the molecular probes, we detected geoA in a wide range of geosmin-producing taxa in both laboratory cultures and environmental samples which provided an important new database of geoA sequences from a diversity of GSM producers, allowing an evaluation of their phylogenetic relationships based on this gene. This study indicated that potential geosmin producers in the Laurentian Great Lakes water and sediment samples are predominantly represented by members of the Nostocaceae, and in particular Anabaena spp. No correlations were apparent between gene expression and ambient temperature, nutrient concentrations and total phytoplankton biomass. Rather, evaluation of the geoA expression pattern in the environmental samples tested from various watersheds and seasons suggested constitutive gene expression.     

Biohydrogen production by novel cyanobacterial strains isolated from rice paddies in Kazakhstan

A limited supply of oil prompts the search for non-traditional energy sources to replace traditional ones. This makes hydrogen gas an appealing alternative source. Photosynthetic organisms capture sunlight very efficiently and convert it into organic molecules. A promising wild strain was isolated for the first time, from the rice paddies of Kazakhstan (Kyzylorda and Almaty regions), which can be considered as one of the most active hydrogen producers compared to the literature. The result showed that among the 13 isolated and collection cyanobacterial strains, Synechocystis sp. S-1 is the most active H₂ producer (2.35 μmol H₂ mg^?1 Chl a h^?1) in the light. In contrast, the wild-type cyanobacterium Anabaena variabilis A-1 had higher productivity, nitrogenase activity, and a stronger capacity to produce hydrogen in the dark (8.67 μmol H₂ mg^?1 Chl a h^?1), which matched the maximum yield obtained in the research. The metabolic modulation performed significantly increased hydrogen production. The highest photohydrogen production rate was observed in cells incubated with 25 μmol HEPES and 50 μmol sodium bicarbonate (NaHCO₃).     

Microcosm experiments of oil degradation by microbial mats. II. The changes in microbial species

The influence of microbial mats on the degradation of two crude oils (Casablanca and Maya) and the effect of oil pollution on the mat structure were assessed using model ecosystems, prepared under laboratory conditions subject to tidal movements, from pristine Ebro Delta microbial-mat ecosystems. Both selected oils are examples of those currently used for commercial purposes. Casablanca crude oil is aliphatic with a low viscosity; Maya represents a sulphur-rich heavy crude oil that is predominantly aromatic. In the unpolluted microcosms, Microcoleus chthonoplastes-, Phormidium- and Oscillatoria-like were the dominant filamentous cyanobacterial morphotypes, whilst Synechoccocus-, Synechocystis- and Gloeocapsa-like were the most abundant unicellular cyanobacteria. After oil contamination, no significant changes of chlorophyll a and protein concentrations were observed, though cyanobacterial diversity shifts were monitored. Among filamentous cyanobacteria, M. chthonoplastes-like morphotype was the most resistant for both oils, unlike the other cyanobacteria, which tolerated Casablanca but not Maya. Unicellular cyanobacteria seemed to be resistant to pollution with both essayed oils, with the exception of the morphotype resembling Gloeocapsa, which was sensitive to both oils. The crude-oil addition also had a significant effect on certain components of the heterotrophic microbial community. Casablanca oil induced an increase in anaerobic heterotrophic bacteria, whereas the opposite effect was observed in those heterotrophs when polluted with Maya oil. The overall results, microbiological and crude-oil transformation analysis, indicate that the indigenous community has a considerable potential to degrade oil components by means of the metabolic cooperation of phototrophic and heterotrophic populations.     

An analysis of satellite-derived chlorophyll and algal bloom indices on Lake Winnipeg

Lake Winnipeg has experienced dramatic increases in nutrient loading and phytoplankton biomass over the last few decades, accompanied by a marked shift in community composition towards the dominance of cyanobacteria. Comprehensive lake-wide observations of algal blooms are critical to assessing the lake's health status, its response to nutrient management practices, and an improved understanding of the processes driving blooms. We present an analysis of the spatial and temporal variability of algal blooms on Lake Winnipeg using satellite-derived chlorophyll and indices for algal bloom intensity, spatial extent, severity, and duration over the period of ESA's MERIS mission (2002–2011). Imagery documented extensive blooms covering as much as 93% of the lake surface. Bloom conditions were analysed in the context of in-lake and watershed processes to gain further insight on the drivers of bloom events. Day to day bloom variability was driven primarily by intermittent wind mixing events, with quiescent periods leading to the formation of dense surface blooms. Seasonal bloom distribution was consistent with light limitation in the south basin and lake circulation transporting bloom material towards the north-east shore. Inter-annual variability in average bloom severity was related to both total phosphorus (TP) loadings and summer lake surface temperatures. Results provide a valuable historical time series of bloom conditions to which ongoing observations from Sentinel-3's OLCI sensor can be added for longer term monitoring and change detection.     

A novel method for tracking western Lake Erie Microcystis blooms, 2002–2011

After a period of improvement from the late 1970s through the mid 1990s, western Lake Erie has returned to eutrophic conditions and harmful algal blooms now dominated by the cyanobacterium Microcystis aeruginosa. The detection of long-term trends in Microcystis blooms would benefit from a convenient method for quantifying Microcystis using archived plankton tows. From 2002 to 2011, summer Microcystis blooms in western Lake Erie were quantified using plankton tows (N = 649). A flotation separation method was devised to quantify Microcystis biovolume in the tows, and the method was tested against whole water cell counts. Floating Microcystis biovolume (mL) in preserved tows was highly correlated with total Microcystis cells (R² = 0.84) and biomass (R² = 0.95) in whole water samples. We found that Microcystis annual biovolume was highly variable among years; the 2011 bloom was 2.4 times greater than the second largest bloom (2008) and 29.0 times greater than the smallest bloom (2002). Advantages of the method include use of archived samples, high sampling volume, and low effort and expense. Limitations include specificity for cyanobacterial blooms dominated by large Microcystis colonies and the need for site-specific validation. This study indicates that the flotation method can be used to rapidly assess past and present Microcystis in western Lake Erie and that there was high variability in the timing, duration, and intensity of the annual Microcystis blooms over a 10-year period. The data made possible by this method will aid further investigations into the underlying causal factors of blooms.     

The ecological history of Lake Erie as recorded by the phytoplankton community

Lake Erie's water quality has fluctuated since European settlement due to cultural eutrophication and the effects of invasive species. Our attempts to understand the cause-and-effect linkages between observed ecosystem changes and various stressors are evolving. Non-indigenous species, pollutants, land-use and climate change that can alter a lake's physical and chemical environment can manifest rapid changes in community composition and abundance of phytoplankton. As such, for many decades researchers have used phytoplankton data from Lake Erie to track environmental changes. We provide a chronological account of previous and ongoing assessments of pelagic algae to summarize past and present environmental conditions of Lake Erie. This review necessarily focuses on diatom-based assessments as their preserved remains in sediments have been used to hind-cast human-induced impacts and recovery. Because of their uniqueness, this review summarizes where possible the long-term trends according to the western, central and eastern lake basins. Overall, this historical assessment summarizes a period of significant eutrophication throughout most of the 20th century, followed by water quality improvement due to nutrient reductions and establishment of filter-feeding dreissenids. Recent data suggest new issues associated with blooms of diatoms and blue-green algae. The challenges facing Lake Erie underline the need for continued monitoring and evaluation of historical records that will help us distinguish natural from anthropogenic changes, and to reveal the causes and extent of environmental insults in order to make management decisions.     

The establishment of the nuisance cyanobacteria Lyngbya wollei in Lake St. Clair and its potential to harbor fecal indicator bacteria

Lyngbya wollei is a filamentous cyanobacterium which forms large nuisance mats and has infested eastern and southeastern U.S. Lakes and reservoirs for over 100 years. Lyngbya was recently identified in the Great Lakes system in the St. Lawrence River, and Western Lake Erie. Here we report on large deposits of L. wollei washing onshore at a popular recreational beach in Lake Saint Clair, part of the Great Lakes system. The amount of L. wollei deposited on shore was quantified and evaluated for the presence of fecal indicator bacteria (FIB). High concentrations of Escherichia coli, enterococci and Clostridium perfringens were found in the L. wollei in nearshore waters. The densities of E. coli (MPN), enterococci (MPN) and C. perfringens (CFU) attached to L. wollei averaged 3.5, 3.2 and 3.2 log/g, respectively. In contrast, nearshore waters contained nearly 10 times less FIB, averaging 2.6, 2.4 and 2.6 log/100 ml of E. coli (MPN), enterococci (MPN) and C. perfringens (CFU), respectively. DNA fingerprint analysis was used to examine the population structure of E. coli isolates obtained from L. wollei mats. The L. wollei-borne E. coli strains were genetically diverse, suggesting a causal relationship between E. coli and L. wollei. Results from this study indicate that in addition to the macroalga such as Cladophora, cyanobacteria like L. wollei also harbor FIB, potentially impacting water quality and human health in the Great Lakes.