Expression of green fluoscrescent protein gene in Sclerotinia sclerotiorum

Protoplasts of the pathogenic plant fungus,Sclerotinia sclerotiorum,were transformed using the pPGF plasmid,which contains green fluorescent protein gene,under the control of Aspergillus nidulans regulatory sequences. The pPGF plasmid was introduced by PEG/CaCl2 treatment. Positive transformants were harvested with hygromycin B (HYG) resistance as selective marker,and then were observed with green fluorescence phenomena in response to blue light,which suggested that GFP gene was cloned into genome DNA of S. sclerotiorum. The transformants were verified mitotically stable by Southern blotting analysis and passage culturing. This study is developed as an initial step for further research into infection mechanisms of S. sclerotiorum to plants and interactions with bio-control fungus.     

小波变换在求解重力测定反问题中的应用

针对重力测定反问题,提出将其离散为线性不适定问题,利用小波变换方法进行数值求解.该方法将小波变换和正则化方法相结合,选取小波函数作为一组基底,将原不适定问题转化为粗子空间上的适定问题,并给出选取粗子空间基的方法.通过数值模拟已有方法和小波变换方法求解结果的比较,表明了小波变换方法的可行性和有效性.     

红外与可见光复合寻的制导中的快速图像配准方法

对现有多传感器图像配准方法在红外与可见光复合寻的制导应用中存在的计算量大等不足,提出了一种基于部分Hausdorff距离的快速方法和一种两集合相似度的定义,从而实现了有效减小误匹配特征点对的红外与可见光图像快速配准方法.此算法通过分别提取两幅图像边缘上显著特征点,再根据提出的快速方法和两集合相似度定义,粗略估计出图像间的旋转角度、平移量;然后依据此估计值,缩小特征点匹配时匹配特征点搜索范围,求得一致特征点对;最后用最小二乘法求解最优变换参数.实验结果表明了该方法的有效性、快速性.     

基于XML的UML模型向AADL模型的自动转换

UML已经成为复杂系统建模的工业标准,并可借助代码自动生成工具实现从分析到编码的开发过程自动化;AADL具有精确的语义和严格的语法规范,可用于描述嵌入式实时系统的软、硬件体系结构,并能分析系统的功能及非功能属性。 UML和AADL模型的基本元对象有相互对应关系,并且两种模型的表示都能够采用标准的XML的交换格式。为充分发挥两种语言的优势,文中研究了UML模型向AADL模型的自动转换,以XML为媒介实现两种模型的元对象的对应转换,最终达到两种模型的转换,使用户在设计阶段能够结合运用AADL、UML工具的优点对系统进行分析。     

Oxidation Of Iron And Manganese By Ozone

The oxidation of iron and manganese by ozone was studied in the laboratory. Model waters both with and without organic matter were used. Results showed iron to be very rapidly oxidized to an insoluble form in the absence of organic matter. However, in the presence of organic matter the iron was protected from oxidation by ozone and precipitation. The degree to which this occurred depended on the nature of the organic matter and the chemical environment at the time of mixing the iron stock and the dissolved organic matter.

Experiments with manganese allowed the determination of second order rate constants for the reaction of ozone with manganese at various pH values. The oxidation of manganese in the presence of organic matter occurred in competition with oxidation of the organic matter. As a result, high ozone doses were required to achieve the same degree of removal of manganese. An increase in bicarbonate alkalinity from 50 mg/L to 200 mg/L did not result in an acceleration of the manganese oxidation in the absence of organic matter. However, in the presence of organic matter, higher levels of bicarbonate created conditions that resulted in more complete oxidation of the manganese following total consumption of the dosed ozone.     

Behavior Of Glyoxal During Granular Activated Carbon Treatment

Glyoxal, which was the strong mutagen formed by ozonation of humic substances, was treated with granular activated carbon (GAC) and the behavior of glyoxal was investigated. Glyoxal itself was adsorbed well on GAC at pH 5–7. But, glyoxal increased by GAC treatment of ozonated humic substances when the ozone dose was insufficient to decolorize the humic substances. The precursors of glyoxal may be among other ozonated products which may be changed to glyoxal on GAC.     

A synthetical development approach for rehabilitation assistive smart product–service systems: A case study

Digital transformation (DT), the combination of information, computing, communication and connectivity technologies, which has triggered an effective upgrade of different aspects of market strategy, customer experience etc. Nowadays, rehabilitation assistive devices (RADs) are evolving to be more digital, intelligent and personalized. Digitalization and servitization have fostered to an emerging business model—the smart product–service system (Smart PSS). Therefore, DT of the RADs’ industry advocates not only the design of products and functions, the more important is the management of service processes and resource integration. With the increase in the elderly and disabled population, the requirement for RADs is becoming more urgent. However, research on Smart PSS for RAD is still limited. The rehabilitation assistive smart product–service systems (RASPSS) was introduced into the development of RADs based on the “Design and Management of DT” strategy through the service design of assistive devices and user requirements analysis. Further, an integrated design of RAD and Smart PSS has been created, a development method of RASPSS proposed, the theoretical model of the Smart PSS based on RADs built. To specify the service framework, this case study discusses the development of a home rehabilitation assistive system for femoral stem fracture patients. This paper evaluates the usability of the system, the results of which prove usability and effectiveness of the RASPSS development method. The RASPSS development model is designed to meet needs of stakeholders, improve the user rehabilitation experience, promote the service innovation of Smart PSS, bring certain market benefits of rehabilitation aids and create social value.     

浅议高职院校科研成果转化问题

当前,高职院校科研工作发展迅速,但是往往"重研究,轻应用",科研成果转化数量少,这是一种资源的浪费。高职院校科研成果转化具有重要的现实意义,应该得到大力发展。文章分析了当前高职院校科研成果转化存在的问题,提出了促进高职院校科研成果转化的对策建议。     

TWO KINDS OF VARIANT IN SHAPE MEMORY MATERIAL

In shape memory materials,that have been trained to have a two way shape memory effect(TWSM),themartensitic variants are classified into two groups,i.e.,preferentially oriented variants and self-accommodatingvariants.Applied stress may promote or constrain the transition of preferentially oriented variants and so changetransformation temperatures but has no essential effect on self-accommodating variants.According to the pointof view mentioned above,some experimental phenomena during thermocycling of a TWSM device may be ex-plained,such as the absence of synchronization between the plot of resistance change.temperature and the plotof memory strain vs.temperature.     

马氏体相变研究的进展和瞻望SCIEI

叙述马氏体相变研究的一些进展和瞻望,包括马氏体相变的定义,马氏体相变热力学,奥氏体状态对马氏体相变的影响,动力学,形核和长大,以及马氏体相变晶体学。