Downregulation of miR-122-5p Activates Glycolysis via PKM2 in Kupffer Cells of Rat and Mouse Models of Non-Alcoholic Steatohepatitis

Non-alcoholic steatohepatitis (NASH) has pathological characteristics similar to those of alcoholic hepatitis, despite the absence of a drinking history. The greatest threat associated with NASH is its progression to cirrhosis and hepatocellular carcinoma. The pathophysiology of NASH is not fully understood to date. In this study, we investigated the pathophysiology of NASH from the perspective of glycolysis and the Warburg effect, with a particular focus on microRNA regulation in liver-specific macrophages, also known as Kupffer cells. We established NASH rat and mouse models and evaluated various parameters including the liver-to-body weight ratio, blood indexes, and histopathology. A quantitative phosphoproteomic analysis of the NASH rat model livers revealed the activation of glycolysis. Western blotting and immunohistochemistry results indicated that the expression of pyruvate kinase muscle 2 (PKM2), a rate-limiting enzyme of glycolysis, was upregulated in the liver tissues of both NASH models. Moreover, increases in PKM2 and p-PKM2 were observed in the early phase of NASH. These observations were partially induced by the downregulation of microRNA122-5p (miR-122-5p) and occurred particularly in the Kupffer cells. Our results suggest that the activation of glycolysis in Kupffer cells during NASH was partially induced by the upregulation of PKM2 via miR-122-5p suppression.     

一种粘滞阻尼体系阻尼矩阵识别新方法

由不同材料组成的混合结构,其阻尼矩阵不再满足与质量矩阵、刚度矩阵的比例关系,具有非比例特性。我国设计规范中常用的振型分解反应谱法是基于比例阻尼建立的,并不严格适用于计算非比例阻尼体系的地震作用效应。该文在基于等效黏性阻尼模型的复模态叠加法基础上,结合虚拟激励法和平稳随机理论,推导了基于等效黏性阻尼模型的反应谱CCQC(Complex Complete Quadratic Combination)法实数表达式,建立了一种解决非比例阻尼混合结构的地震作用效应计算问题的理论方法。算例分析表明：与基于分块Rayleigh阻尼模型的反应谱CCQC法相比,该文建立的基于等效黏性阻尼模型的反应谱CCQC法直接依赖于结构的质量、刚度和阻尼参数,不受振型组合的影响,具有计算结果唯一的优点,且仅涉及位移相关系数的计算;与基于能量法的反应谱CQC法相比,基于等效黏性阻尼模型的反应谱CCQC法可有效考虑结构的非比例特性,适用于非比例特性较强的混合结构。     

环江油田巴19区块长7段钙夹层评价与大斜度井分段压裂优化

为实现最大化油藏接触,提高油藏产出水平,长庆环江油田巴19区块长7油藏采用大斜度井开发方式。在初期压裂改造中,由于忽视了层内钙质夹层对压裂缝高的影响,导致实际裂缝的几何形态与设计产生巨大偏差,出现了邻井裂缝串通及生产高含水等问题。本文通过研究油藏剖面测井特征,在细分层位基础上分析纵向应力分布,研究“复合层效应”影响裂缝缝高延伸的关键因素,优化了分段压裂方案,获得了更好的水力裂缝几何形态和尺寸。技术调整后现场压裂工艺质量获得提高,长7段油藏压裂改造质量优良率100%,大大提高了该区块的开发水平。     

低透气性煤层高压水力压裂—冲孔联合增透技术研究及应用

针对深部开采煤层普遍存在的构造应力复杂、透气性系数低、抽采效果差等问题,为探究最优的瓦斯抽采技术方案,以水溪煤矿K₁松软煤层为研究对象,分别采用水力压裂、水力冲孔、普通抽采和高压水力压裂—冲孔联合增透4种技术,进行现场对比应用试验。结果表明：高压水力压裂—冲孔联合增透技术对煤层透气性系数的提高程度分别为水力压裂、水力冲孔和普通抽采方案的1.85、1.61、6.30倍,抽采瓦斯纯流量分别提高了1.42、1.33、2.32倍,此外,抽采汇总瓦斯浓度(CH₄体积分数)保持在42.6%以上,抽采效率为四者之中最高。现场应用结果表明,高压水力压裂—冲孔联合增透技术具有明显的技术优势。     

不规则钢筋砼板的塑性理论计算

不规则钢筋砼板的计算,目前尚无明确的方法和统一的计算结果。本探讨不规则的钢筋砼板在极限状态下承载能力的计算方法。用屈服线分析法求板的承载能力的数值解,对角部效应的影响提出了补偿内力功的概念,采用局部配置板顶钢盘的方法。     

Performances of carbon nanotube field effect transistors with altered channel length

The influence of channel length on the performances of carbon nanotube field effect transistors(CNT-FETs) has been studied.Buffered oxide etching was used to remove approximately a 60 nm layer from the original 100 nm silicon dioxide layer,to thin the dielectric layer of the back gate.Channel length of the CNT-FETs was changed along with the etching process.The dependence of drain-source current on gate voltage was measured to analyze the performance of the CNT-FETs,including the transconductance,carrier mo...     

麦秸秆加筋盐渍土的尺寸效应与制样问题处置

制备均匀且整体性较好的试样是保证室内实验结果可靠的前提条件。提出了两端压实的静力制样法,围绕制样中出现的几个问题,制定了适宜的应对措施。同时完成了不同直径的盐渍土和麦秸秆加筋土试样的抗压强度实验,分析了试样抗压强度的尺寸效应,以及试样直径与加筋条件间的比例关系。结果显示:采用两端压实的静力制样方法制备加筋土试样是可行的;两种试样均存在明显的尺寸效应;由于麦秸秆的加筋作用,使得麦秸秆加筋土不同尺寸试样的抗压强度尺寸效应大于盐渍土的,且其抗压强度的尺寸效应随试样尺寸的增大而越来越明显;适宜加筋长度为0.316倍试样直径,适宜加筋率0.25%。研究结果可为制备合格的加筋土试样提供参考,也有益于在未来的研究中选择更合理的麦秸秆加筋条件。