牛α-乳白蛋白-油酸复合物对肿瘤细胞能量代谢的影响

本研究以人肝癌细胞(HepG2)为对象,分为α-LA-OA处理组、牛-α乳白蛋白(α-LA)处理组和溶剂对照组,以细胞的糖酵解和线粒体有氧呼吸2个过程中胞外酸化率和细胞耗氧率的变化水平为衡量指标,评估α-LA-OA对肿瘤细胞能量代谢的影响.结果表明,α-LA-OA能够显著性降低人肝癌细胞的糖酵解能力以及线粒体有氧呼吸能...     

徐坤学人小说论

以学者和女性的双重身份出发,探讨了徐坤的小说在内容、女性视角、语言风格三个方面区别于其他学人小说的特征。     

从时间维度出发的建筑设计

本文讨论了从时间维度出发进行建筑设计的方法及可能性。主要分析了建筑师、感受主体、感受客体之间的相互关系,提出了从时间维度出发进行建筑设计的几个主要步骤,并举具体实例分析不同情况下,考察时间段和考察要素的不同。为建筑创作提供了一些新的思路。     

Comparative Antimicrobial Activity of Silver Nanoparticles Obtained by Wet Chemical Reduction and Solvothermal Methods

The synthesis of nanoparticles from noble metals has received high attention from researchers due to their unique properties and their wide range of applications. Silver nanoparticles (AgNPs), in particular, show a remarkable inhibitory effect against microorganisms and viruses. Various methods have been developed to obtain AgNPs, however the stability of such nanostructures over time is still challenging. Researchers attempt to obtain particular shapes and sizes in order to tailor AgNPs properties for specific areas, such as biochemistry, biology, agriculture, electronics, medicine, and industry. The aim of this study was to design AgNPs with improved antimicrobial characteristics and stability. Two different wet chemical routes were considered: synthesis being performed (i) reduction method at room temperatures and (ii) solvothermal method at high temperature. Here, we show that the antimicrobial properties of the obtained AgNPs, are influenced by their synthesis route, which impact on the size and shape of the structures. This work analyses and compares the antimicrobial properties of the obtained AgNPs, based on their structure, sizes and morphologies which are influenced, in turn, not only by the type or quantities of precursors used but also by the temperature of the reaction. Generally, AgNPs obtained by solvothermal, at raised temperature, registered better antimicrobial activity as compared to NPs obtained by reduction method at room temperature.     

国内外能源利用效率比较及对我国的启示

经济增长与能源消耗之间有着密切的关系。通过对快速增长的新兴经济体以及经历类似阶段的发达国家的能源利用效率的比较和政策的分析,指出我国能源问题的原因在于生活、建筑能耗方面及高耗能行业的过度消耗,进而提出规范市场、优化产业结构等政策建议,从而实现使经济发展走上资源节约型、环境友好型的良性循环这一目标。     

ALPUR除气装置在25 t熔铸生产线中的应用

25 t燃油熔铝炉熔炼的铝合金熔体中氢含量较高,配备ALPUR除气净化装置,通过合理使用,达到了满意的除氢效果,保证了铸锭的内部质量。     

相干态光场的位相涨落

根据Pegg-Barnett位相理论,推导出相干态光场的位相概率分布函数,计算出位相涨落,并利用MATHEMATICA对相干态光场的位相函数和位相涨落进行了数值模拟,画出了示意图.结果表明位相函数同时受e指数函数和幂指数函数的制约,从均匀分布到泊松分布到均匀分布,直至呈现零分布;在位相变化的一个周期内,相干态位相分布曲线出现涨落,涨落同时受参考位相和相干系数的影响,随着n值的增加,曲线趋向于高斯分布.     

大型超市能耗动态模拟研究

建立了大型超市能耗动态模拟的计算模型。以上海某大型超市为例,应用DeST软件模拟和实际调研分析相结合的方法,对该大型超市的全年逐时能耗进行了模拟,并利用模拟结果分析了该大型超市的能耗现状和结构。利用该建筑实测能耗数据对模拟结果进行了验证,表明该计算模型准确合理。     

The Effect of High and Variable Glucose on the Viability of Endothelial Cells Co-Cultured with Smooth Muscle Cells

Diabetes mellitus causes endothelial dysfunction. The aim of this study was to investigate the effect of normal (5 mmol/L), high (20 mmol/L), and fluctuating (5 and 20 mmol/L changed every day) glucose concentration in the culture medium on the viability of human umbilical vein endothelial cells (HUVECs) co-cultured with human umbilical artery smooth muscle cells (HUASMCs). The cultures were conducted on semi-permeable flat polysulfone (PSU) fibronectin-coated membranes immobilized in self-made inserts. The insert contained either HUVECs on a single membrane or HUASMCs and HUVECs on two membranes close to each other. Cultures were conducted for 7 or 14 days. Apoptosis, mitochondrial potential, and the production of reactive oxygen species and lactate by HUVECs were investigated. The results indicate that fluctuations in glucose concentration have a stronger negative effect on HUVECs viability than constant high glucose concentration. High and fluctuating glucose concentrations slow down cell proliferation compared to the culture carried out in the medium with normal glucose concentration. In conclusion, HUASMCs affect the viability of HUVECs when both types of cells are co-cultured in medium with normal or variable glucose concentration.     

Chl1, an ATP-Dependent DNA Helicase,Inhibits DNA:RNA Hybrids Formation at DSB Sites to Maintain Genome Stability in S. pombe

As an ATP-dependent DNA helicase, human ChlR1/DDX11 (Chl1 in yeast) can unwind both DNA:RNA and DNA:DNA substrates in vitro. Studies have demonstrated that ChlR1 plays a vital role in preserving genome stability by participating in DNA repair and sister chromatid cohesion, whereas the ways in which the biochemical features of ChlR1 function in DNA metabolism are not well understood. Here, we illustrate that Chl1 localizes to double-strand DNA break (DSB) sites and restrains DNA:RNA hybrid accumulation at these loci. Mutation of Chl1 strongly impairs DSB repair capacity by homologous recombination (HR) and nonhomologous end-joining (NHEJ) pathways, and deleting RNase H further reduces DNA repair efficiency, which indicates that the enzymatic activities of Chl1 are needed in Schizosaccharomyces pombe. In addition, we found that the Rpc37 subunit of RNA polymerase III (RNA Pol III) interacts directly with Chl1 and that deletion of Chl1 has no influence on the localization of Rpc37 at DSB site, implying the role of Rpc37 in the recruitment of Chl1 to this site.